Sperm cell biology: current perspectives and future prospects

Aitken, R. John; Henkel, Ralf

doi:10.1038/aja.2010.155

Cited by 16 publications

(12 citation statements)

References 16 publications

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“…This result was similar to those reported in studies suggesting an association between the two types of DNA damage; it supports the notion that ROS can reach DNA nucleotides leading to DNA fragmentation [46, 47]. The proposed mechanism for ROS induced DNA fragmentation is such that formation of 8-oxo-dGuo leads to the creation of basic sites and destabilization of the DNA backbone [50]. The DNA fragmentation was found to discrete into smaller fragments in the 20-25 kb range rather than much larger 50-kb toroid fragments detected by other studies [48,49].…”

Section: Discussionsupporting

confidence: 88%

Sperm DNA oxidative damage and DNA adducts

Jeng

Pan

Chao

et al. 2015

Mutation Research/Genetic Toxicology and Environmental Mutagene

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The objective of this study was to investigate DNA damage and adducts in sperm from coke oven workers who have been exposed to polycyclic aromatic hydrocarbons. A longitudinal study was conducted with repeated measurements during spermatogenesis. Coke-oven workers (n=112) from a coke-oven plant served the PAH-exposed group, while administrators and security personnel (n=67) served the control. Routine semen parameters (concentration, motility, vitality, and morphology) were analyzed simultaneously; the assessment of sperm DNA integrity endpoints included DNA fragmentation, bulky DNA adducts, and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dGuo). The degree of sperm DNA fragmentation was measured using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay and sperm chromatin structure assay (SCSA). The PAH-exposed group had a significant increase in bulky DNA adducts and 8-oxo-dGuo compared to the control subjects (Ps = 0.002 and 0.045, respectively). Coke oven workers' percentages of DNA fragmentation and denaturation from the PAH-exposed group were not significantly different from those of the control subjects (Ps = 0.232 and 0.245, respectively). Routine semen parameters and DNA integrity endpoints were not correlated. Concentrations of 8-oxo-dGuo were positively correlated with percentages of DNA fragmentation measured by both TUNEL and SCSA (Ps = 0.045 and 0.034, respectively). However, the concentrations of 8-oxo-dGuo and percentages of DNA fragmentation did not correlate with concentrations of bulky DNA adducts. In summary, coke oven workers with chronic exposure to PAHs experienced decreased sperm DNA integrity. Oxidative stress could contribute to the degree of DNA fragmentation. Bulky DNA adducts may be independent of the formation of DNA fragmentation and oxidative adducts in sperm. Monitoring sperm DNA integrity is recommended as a part of the process of assessing the impact of occupational and environmental toxins on sperm.

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Section: Discussionsupporting

confidence: 88%

Sperm DNA oxidative damage and DNA adducts

Jeng

Pan

Chao

et al. 2015

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…Novel tests are emerging from andrology research laboratories that may soon become clinically available. The 'omics revolution, as it is termed, refers to the study of genes (genomics), transcripts (transcriptomics), proteins (proteomics) and the various metabolites (metabolomics) 55. With these technologies, inventories of lipids, proteins, metabolites and RNA species may be determined.…”

Section: Introductionmentioning

confidence: 99%

An update on the clinical assessment of the infertile male

Esteves¹,

Miyaoka²,

Agarwal

2011

Clinics

254

236

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Male infertility is directly or indirectly responsible for 60% of cases involving reproductive-age couples with fertility-related issues. Nevertheless, the evaluation of male infertility is often underestimated or postponed. A coordinated evaluation of the infertile male using standardized procedures improves both diagnostic precision and the results of subsequent management in terms of effectiveness, risk and costs. Recent advances in assisted reproductive techniques (ART) have made it possible to identify and overcome previously untreatable causes of male infertility. To properly utilize the available techniques and improve clinical results, it is of the utmost importance that patients are adequately diagnosed and evaluated. Ideally, this initial assessment should also be affordable and accessible. We describe the main aspects of male infertility evaluation in a practical manner to provide information on the judicious use of available diagnostic tools and to better determine the etiology of the most adequate treatment for the existing condition.

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“…The crucial thing is spermatozoa's ability to fertilize an oocyte regardless of the sperm number and motility, as ~15% men with fertility problem are normozoospermic patients (Agarwal & Allamaneni, ). Spermatozoa are a highly specialized cell undergoing various biological changes necessary to achieve fertilization (Aitken & Henkel, ). Although, there is no direct measurement of sperm fertilization capability, different assays were developed for better understanding of male fertility pathophysiology and aetiology mostly in 1980s.…”

Section: Introductionmentioning

confidence: 99%

Sperm DNA integrity testing: big halo is a good predictor of embryo quality and pregnancy after conventional IVF

et al. 2014

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SUMMARYSperm DNA integrity is a sperm functional parameter of male fertility evaluation. Two parameters of sperm DNA integrity were observed: DNA damage expressed as DNA fragmentation index (DFI) and percentage of the DNA undamaged spermatozoa expressed as big halo. Halosperm test was used for sperm DNA integrity determination. The aim of this study was to evaluate which DNA integrity parameter is better as an embryo quality and pregnancy prognostic parameter after the conventional IVF. We evaluated two embryo groups (positive and negative group) according to the 3rd day cumulative embryo score. Big halo and DFI, as we expected, showed good correlation (r = À0.69; p < 0.001). Receiver operating characteristic (ROC) analyses show that DFI and big halo are significant (p < 0.001) as prognostic parameters of embryo quality. ROC curves comparison of DFI and big halo revealed the AUC value for big halo to be significantly higher (DFI AUC = 0.71 vs. big halo AUC = 0.83; p = 0.025) than for DFI. Big halo was found to be the only independent predictor of embryo quality. Sperm DNA integrity both parameters are good prognostic parameters of embryo quality after the conventional IVF where big halo seems to be better. ROC analyses show DFI and big halo as significant prognostic parameters for achieved pregnancy (AUC AE SE for DFI was 0.67 AE 0.06 and 0.75 AE 0.06 for big halo). To our knowledge, this is the first study demonstrating the correlation between sperm DNA undamaged rate expressed as big halo parameter and semen characteristics as well as the influence on fertilization rate, embryo quality and pregnancy in conventional IVF.

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Sperm cell biology: current perspectives and future prospects

Cited by 16 publications

References 16 publications

Sperm DNA oxidative damage and DNA adducts

Sperm DNA oxidative damage and DNA adducts

An update on the clinical assessment of the infertile male

Sperm DNA integrity testing: big halo is a good predictor of embryo quality and pregnancy after conventional IVF

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