Sperm cell purification from mock forensic swabs using SOMAmer™ affinity reagents

Katilius, Evaldas; Carmel, Andrew B.; Koss, Heidi; O'Connell, Dan; Smith, Breanna C.; Sanders, Glenn M.; LaBerge, Greggory S.

doi:10.1016/j.fsigen.2018.03.011

Cited by 16 publications

(8 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Katilius et al developed an affinity-based sperm purification method using magnetic streptavidin-coated beads with bound ligands modified using biotin. The epithelial and sperm cell mixtures were incubated with these beads to bind the sperm cells, with the epithelial DNA being removed . Microfluidics has also been used for differential extraction.…”

Section: Dna Extraction and Sample Recoverymentioning

confidence: 99%

Forensic DNA Analysis

McCord

Gauthier

Cho³

et al. 2018

Anal. Chem.

View full text Add to dashboard Cite

Section: Dna Extraction and Sample Recoverymentioning

confidence: 99%

Forensic DNA Analysis

McCord

Gauthier

Cho³

et al. 2018

Anal. Chem.

View full text Add to dashboard Cite

“…Since antibody‐based affinity purification has a relatively low recovery of sperm (Li et al, 2014; Y. Xu et al, 2016; Zhao et al, 2016), Slow Off‐rate Modified Aptamers (SOMAmers) were investigated as alternative affinity ligands to bind sperm (Katilius et al, 2018). In samples with abundant sperm, these synthetic aptamers with higher affinities than antibodies (Lollo, Steele, & Gold, 2014) proved to be as effective as standard DE in separating sperm from epithelial cells (Katilius et al, 2018). In samples with low sperm concentrations (less than 1:20 diluted semen), this method was less effective, likely due to general binding of the aptamers to the sperm tail.…”

Section: Isolation Of Male Dna In Sexual Assault Cases—alternatives Amentioning

confidence: 99%

“…In samples with low sperm concentrations (less than 1:20 diluted semen), this method was less effective, likely due to general binding of the aptamers to the sperm tail. In contrast, male DNA could be successfully recovered from even 1:640 semen dilutions by DE as the method was independent of the presence/absence of the sperm tail (Katilius et al, 2018). This suggests that the binding location of the ligand is more important than its binding strength in obtaining a higher yield of sperm.…”

Section: Isolation Of Male Dna In Sexual Assault Cases—alternatives Amentioning

confidence: 99%

Recent trends and developments in forensic DNA extraction

Chong

Thong

Syn

2020

WIREs Forensic Science

View full text Add to dashboard Cite

The extraction of DNA is a fairly standard procedure routinely undertaken in biological academic and research settings. In a forensic DNA context, DNA extraction is the crucial first step toward the generation of DNA profiles used to support convictions or exonerations in the criminal justice system. However, it is often complicated by the additional challenge of yielding sufficient quantities of clean DNA that is free from environmental inhibitors, from a wide spectrum of sample types which may contain limited quantities of biological material. While improvements in methods and technology over the decades have sped up and simplified the process of DNA extraction, several issues remain to be fully addressed—such as the incomplete separation of spermic DNA from sperm‐epithelial cell mixtures, increasing demands for ever‐faster DNA results, or even on‐scene DNA processing, and processing of difficult samples such as skeletal remains or samples contaminated by Chemical, Biological, or Radiological (CBR) agents. These challenges are often compounded by high work volumes and limited quantities of starting material. As such, this review focuses on recent trends and developments in four areas of forensic DNA extraction: (a) analysis of sexual assault evidence which often represents a significant portion of a forensic DNA laboratory's casework, (b) use of portable rapid DNA instruments which has gained much traction among law enforcement in recent times, (c) DNA extraction from difficult and CBR‐contaminated samples which, albeit rare, would be essential in a DNA laboratory's toolbox in the event of an incident, and (d) bypassing DNA extraction altogether. This article is categorized under: Forensic Biology > Forensic DNA Technologies

show abstract

“…While the majority of applications are performed with single base modified SOMAmers, as is the case for the SOMAscan platform or sandwich assays, the possible modifications and applications of SOMAmers reach far beyond this scope. For example, sandwich assays are described with double base-modified SOMAmers [ 95 ] and SOMAmers can be used for on-chip electrophoretic assays [ 125 ], the development of gravimetric aptasensors [ 126 ] or even for affinity pull-down experiments [ 127 ], highlighting the market potential of aptamers containing modified nucleobases.…”

Section: Strategies For Improving Binding Affinity Through Chemicamentioning

confidence: 99%

Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects

Elskens

Madder

2020

IJMS

116

View full text Add to dashboard Cite

Aptamers are short single stranded DNA or RNA oligonucleotides that can recognize analytes with extraordinary target selectivity and affinity. Despite their promising properties and diagnostic potential, the number of commercial applications remains scarce. In order to endow them with novel recognition motifs and enhanced properties, chemical modification of aptamers has been pursued. This review focuses on chemical modifications, aimed at increasing the binding affinity for the aptamer’s target either in a non-covalent or covalent fashion, hereby improving their application potential in a diagnostic context. An overview of current methodologies will be given, thereby distinguishing between pre- and post-SELEX (Systematic Evolution of Ligands by Exponential Enrichment) modifications.

show abstract

Sperm cell purification from mock forensic swabs using SOMAmer™ affinity reagents

Cited by 16 publications

References 18 publications

Forensic DNA Analysis

Forensic DNA Analysis

Recent trends and developments in forensic DNA extraction

Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects

Contact Info

Product

Resources

About