Sperm chromatin condensation in infertile men with varicocele before and after surgical repair

Sadek, Ahmed M.; Almohamdy, Abdel Shakour A.; Zaki, Amr Mohamed; Aref, Mohamed Ibraheim; Ibrahim, Samir; Mostafa, Taymour

doi:10.1016/j.fertnstert.2011.01.008

Cited by 82 publications

(69 citation statements)

References 38 publications

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“…Concomitantly, the percentage of abnormal forms, DFI and seminal ROS level were higher in the varicocele group. Such observations confirm the detrimental role of varicocele in spermatogenesis [11,15] It is notable that varicocele in our patients was associated with significant lower level of serum omentin-1and slight lower level of seminal omentin-1compared with patients without varicocele. These findings present another mechanism by which varicocele impair spermatogenesis, however, this postulate needs further research that is beyond the scope of this study.…”

Section: Discussionsupporting

confidence: 89%

“…DFI was performed using flowcytometry (DAKOCytomation, Glostrup, Denmark) by Coulter (Beckman Coulter, Fullerton, CA) based on the fluorescence emission from sperms stained with propidium iodide (PI) and excitation with a 488-nanometer (nm) argon laser [15] . Fresh semen samples were diluted with phosphate-buffered saline (PBS) to 2x10 6 sperm/mL.…”

Section: Sperm Dna Fragmentation Index Assessment (Dfi)mentioning

confidence: 99%

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Omentin-1 in serum and seminal plasma correlate with semen quality

et al. 2017

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Introduction: Adipokines are differently regulated in the male reproductive tract and they were reported to affect sperm functionality. Serum omentin-1 was reported to have a range of effects on various cell types. Aim: To explore the presence of omentin-1in human semen, to investigate its relation with semen parameters in both fertile and infertile men and to compare its serum and seminal plasma levels in particular conditions such as; smoking, varicocele and leukocytospermia. Methods: Thirty nine infertile and 26 fertile men were enrolled in this case control study. They were subjected to history taking, clinical/physical examination, semen analysis, and measurement of omentin-1 in serum and seminal plasma, DNA fragmentation index (DFI) and seminal reactive oxygen species (ROS). Results: Omentin-1 was detected in all analyzed semen and serum samples (n=65). The concentration of omentin-1 was significantly higher in seminal plasma than in serum. Serum and seminal omentin-1 were positively correlated with sperm concentration and percentage of progressive motility and were negatively correlated with percentage of abnormal forms, DFI and ROS. The mean seminal plasma level of omentin-1 was significantly decreased in smokers and in patients with leukocytospermia. The mean serum level of omentin-1 was significantly decreased in patients with varicocele. Both serum and seminal omentin-1 levels were significantly lower in infertile patients than fertile men. Conclusions: Semen and serum Omentin-1 correlate with semen quality. In cases of leukocytospermia and smoking, seminal omentin-1 concentrations were decreased, suggesting that it may play a regulatory role in inflammation of the male reproductive system.

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Section: Discussionsupporting

confidence: 89%

Section: Sperm Dna Fragmentation Index Assessment (Dfi)mentioning

confidence: 99%

Omentin-1 in serum and seminal plasma correlate with semen quality

et al. 2017

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“…To validate the observed association between varicocele and sperm DNA fragmentation, several investigators have examined the effect of varicocelectomy on sperm DNA damage. There are now three retrospective and nine prospective studies on the effect of varicocelectomy on sperm DNA damage, and these studies have all shown that varicocele repair is associated with reduced sperm DNA damage (8,33,36,(71)(72)(73)(74)(75)(76)(77)(78)(79) (Table 2). Although there are no randomized controlled trials on the role of varicocelectomy on sperm DNA integrity, the published varicocelectomy studies provide indirect evidence in support of the adverse effect of varicocele on sperm DNA integrity.…”

Section: Discussion: Are Varicoceles Associated With Increased Dna Frmentioning

confidence: 99%

Are varicoceles associated with increased deoxyribonucleic acid fragmentation?

Zini

Dohle

2011

Fertility and Sterility

153

101

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“…Tanaka et al (27) and Sadek et al (28) added that reduced DNA synthesis is one of the causes of spermatogeinc dysfunction in varicocele-associated testes compared with infertile cases devoid of such pathology. Fazlioglu et al (29) showed that after creation of unilateral varicocele, the apoptotic process was increased in both testes and that it decreased markedly after varicocelectomy.…”

Section: Discussionmentioning

confidence: 99%