Sperm chromatin stability and its relationship with fertilization rate after Intracytoplasmic Sperm Injection (ICSI) in an assisted reproduction program

Abstract

Objective Evaluate sperm chromatin stability and its relationship with the rate of fertilization after procedures of intracytoplasmic sperm injection (ICSI) in a program of assisted reproduction. Design Prospective study. Setting Institute of Gynecology and Reproduction. Patients Thirty-three women with their respective partners (12 couples in the study group and 21 couples in the control group) participating in a program of assisted reproduction. The study group was defined as men with >30% of nondecondensed spermatozoa (high sperm chromatin stability). Interventions A part of each seminal sample was used to evaluate sperm chromatin stability under SDS and EDTA treatment and the second aliquot was used for the ICSI procedure. Fertilization was evaluated 16-18 h post sperm injection at a pronuclear stage. The fertilized oocytes were further cultured for 24-48 h before transfer to the patient. Main outcome measures Fertilization rate.Results Thirty-five oocytes (35.7%) in the group of study and 109 oocytes (78.9%) in the control group showed two pronuclei (P<0.001). The coefficient of determination between the SDS+EDTA (Grade 2) and rate of fertilization was r 2 =0.85 (P<0.001) and the coefficient of regression was 1.72±0.19 (β±ES) (P<0.001). Conclusions High sperm chromatin stability is a factor which reduces the rate of fertilization after ICSI procedure.

Semen quality in Peruvian pesticide applicators: association between urinary organophosphate metabolites and semen parameters

“…It is also known that OP may affect DNA integrity [ 13 , 12 ]. If OP affects sex accessory gland functions, then, this may affect sperm DNA [ 42 ]. Effect of OP seems to be accumulative since the single measurement was unable to demonstrate all the reproductive effects observed when variable exposure to pesticides was included in the regression analysis.…”

Effect of sperm DNA fragmentation on clinical outcomes for Chinese couples undergoing in vitro fertilization or intracytoplasmic sperm injection

“…Oocytes were assessed to determine whether fertilization had occurred at 16–18 h after insemination or ICSI. 27 Fertilization was considered to be normal if two pronuclei (PN) and two polar bodies were identified. The fertilization rate was calculated as the percentage of metaphase II oocytes forming two PN.…”

In Vivo Culture System Using the INVOcell Device Shows Similar Pregnancy and Implantation Rates to those Obtained from in Vivo Culture System in ICSI Procedures

“…Five hours after aspiration, the cumulus–oocyte complexes were denuded with 80 UI/mL of hyaluronidase (IVFonline) and the oocytes in metaphase II were injected according to methods previously described. 12 …”