Sperm chromatin structure assay (SCSA): a tool in diagnosis and treatment of infertility

Bungum, Mona; Bungum, Leif; Giwercman, Aleksander

doi:10.1038/aja.2010.73

Cited by 173 publications

(138 citation statements)

References 86 publications

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“…Bull sperm oxidative stress and DNA damage fertility (Liu & Baker 2002, Aitken 2006, Bungum et al 2011, Shamsi et al 2011. Interestingly, sperm motility in this study was not affected by the increase in OS.…”

Section: Tbars Groupsmentioning

confidence: 64%

Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome

Simões¹,

Feitosa²,

Siqueira³

et al. 2013

Reproduction

121

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Sperm chromatin fragmentation may be caused by a number of factors, the most significant of which is reactive oxygen species. However, little is known about the effect of sperm oxidative stress (OS) on DNA integrity, fertilization, and embryonic development in cattle. Therefore, the goal of this study was to evaluate the influence of sperm OS susceptibility on the DNA fragmentation rate and in vitro embryo production (IVP) in a population of bulls. Groups of cryopreserved sperm samples were divided into four groups, based on their susceptibility to OS (G1, low OS; G2, average OS; G3, high OS; and G4, highest OS). Our results demonstrated that the sperm DNA integrity was compromised in response to increased OS susceptibility. Furthermore, semen samples with lower susceptibility to OS were also less susceptible to DNA damage (G1, 4.06%; G2, 6.09%; G3, 6.19%; and G4, 6.20%). In addition, embryo IVP provided evidence that the embryo cleavage rate decreased as the OS increased (G1, 70.18%; G2, 62.24%; G3, 55.85%; and G4, 50.93%), but no significant difference in the blastocyst rate or the number of blastomeres was observed among the groups. The groups with greater sensitivity to OS were also associated with a greater percentage of apoptotic cells (G1, 2.6%; G2, 2.76%; G3, 5.59%; and G4, 4.49%).In conclusion, we demonstrated that an increased susceptibility to OS compromises sperm DNA integrity and consequently reduces embryo quality.

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Section: Tbars Groupsmentioning

confidence: 64%

Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome

Simões¹,

Feitosa²,

Siqueira³

et al. 2013

Reproduction

121

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“…With the advent of modern assisted conception techniques, the role of sperm factor in recurrent assisted conception loss is being realized. Although the threshold, for sperm DFI in infertile men is established [5,8,15,44] the role of sperm factor in iRPL following spontaneous conception has not been extensively evaluated [3,20], and DFI cut -off value has not yet been established. This study has tried to establish a threshold value of sperm DFI in cases with iRPL following spontaneous conception for the first time in India and is amongst the few studies in the world [28,39].…”

Section: Discussionmentioning

confidence: 99%

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Kumar

Deka

Singh

et al. 2012

J Assist Reprod Genet

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Purpose Standard semen parameters are poor predictors of fertility potential. To date, apart from, paternal karyotyping sperm factors are not evaluated in recurrent pregnancy loss (RPL), only recent studies have emphasized the role of sperm factors in early embryonic development as sperm transcribes genes critical for early embryonic development. Sperm DNA integrity is useful diagnostic and prognostic marker and has clinical implications in idiopathic recurrent pregnancy loss (iRPL) following spontaneous conception. The aim of this study was to assess DNA integrity in cases experiencing iRPL following spontaneous conception. Methods Semen samples from 45 patients and 20 controls were analyzed as per WHO 1999 guidelines and sperm chromatin structure assay (SCSA) was used to measure DNA fragmentation index (DFI). Results By applying receiver operating curve (ROC) analysis, sperm DFI of approximately 26 % was found in male partner of couples experiencing iRPL. Conclusions Our data indicate that sperm from men with a history of iRPL have a higher percentage of DNA damage as compared to control group, and this can explain pregnancy loss in these patients. Men with higher DFI are infertile whereas men with lower DFI (26 %) are able to conceive but experience recurrent pregnancy loss. Thus it is important to evaluate sperm DFI in couples experiencing iRPL to understand exact aetiology of RPL and determine prognosis and management.

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“…These markers cannot be detected by routine SA but seems to better correlate with the male fertility status than the latter [52][53][54][55][56][57][58].…”

Section: Current Sperm Function Testsmentioning

confidence: 99%

Engaging Practicing Gynecologists in the Management of Infertile Men

Agarwal

Hamada

Esteves³

2014

J Obstet Gynecol India

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In the modern era, contemporary management of male infertility has undergone groundbreaking changes with the introduction of new concepts, advanced testing, and therapeutic interventions. As practicing gynecologists are often the first physicians who encounter an infertile couple, it is essential that these clinicians are continuously updated about the new pearls and pitfalls of male infertility management. Semen analysis is commonly ordered by gynecologists. In 2010, the WHO released new cutoff reference values for the semen parameters adopting novel methodology, which has incited much debate. Reference values have been lowered in comparison with previous standards, with a direct clinical implication in decision-making strategies. Specialized sperm-function tests, such as sperm oxidative stress and sperm chromatin integrity assessments, became clinically available, thus offering an opportunity to better understand sperm dysfunctions concealed during routine semen analysis. Furthermore, the initial counseling of azoospermic men by an andrologically well educated gynecologist may alleviate the misconception and distress surrounding the false belief of sterility, and will clarify the 123 available options of percutaneous and microsurgical spermretrieval techniques and assisted conception outcome. Regarding varicocele, which is commonly seen in infertile males, it is now clear that the best treatment option for infertile men with clinical varicocele is the microsurgical vein ligation. Natural conception is significantly improved after varicocelectomy, and recent data suggest that such treatment optimizes reproductive outcome of couples undergoing ICSI or micro-TESE sperm retrieval. Lastly, new therapeutic interventions, including oral antioxidant therapy and lifestyle modifications, have gained increasing attention, as they aid in alleviating male infertility.

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Sperm chromatin structure assay (SCSA): a tool in diagnosis and treatment of infertility

Cited by 173 publications

References 86 publications

Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome

Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Engaging Practicing Gynecologists in the Management of Infertile Men

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