Sperm chromatin structure integrity in liquid stored boar semen and its relationships with field fertility

Boe-Hansen, G.; Christensen, Preben; Vibjerg, D.; Nielsen, M. B. F.; Hedeboe, A.M.

doi:10.1016/j.theriogenology.2007.12.004

Cited by 84 publications

(70 citation statements)

References 25 publications

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“…In the present study the percentage of SCI was less than 6% (3.51% and 3.33% for young and old boars, respectively). Moreover, Boe-Hansen et al (2008) demonstrated that diff erences in litter size can be observed with thresholds as low as 2.1% or 3.0% for the DNA fragmentation index in liquid-stored boar semen. In our study no diff erences in litter size were found, but we tested crossbred boars of diff erent ages, while in the previous study Hampshire, Danish Large White and Landrace boars were used.…”

Section: Discussionmentioning

confidence: 97%

Age-related changes in quality and fertility of porcine semen

Tsakmakidis

Khalifa²,

Boscos

2012

Biol. Res.

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The aim of this study was to investigate the eff ect of boar age on quality traits and fertility of liquid-stored semen. Boars were allocated into 3 age groups: 7-10 months (young), 18-33 months (mature), 51-61 months (old). Ejaculates of ≥ 200x10 6 sperm/ml and 85% total motile sperm were extended to 30x10 6 sperm/ml, stored at 17-18 °C and used within 12-24 h for artifi cial insemination (AI) of 2062 multiparous sows. After 24 h of storage, aliquots of diluted semen were assessed for sperm progressive motility (SPM), incidence of sperm chromatin instability (SCI), proportion of live morphologically normal sperm (LMNS) and head morphometry of LMNS. The results showed that young boars had higher percentages of SCI and lower proportions of LMNS than those of the mature (p < 0.05) and old (p < 0.001) boars, respectively. Sperm head dimensions of young and old boars were greater (p < 0.03-0.001) than those of mature boars. The farrowing rate of young boars (65%) was signifi cantly lower (p < 0.001; χ 2 = 30-61) than those of the mature (87.2%) and old (84.7%) boars. The relationship between sperm head dimensions and boar fertility was non-signifi cant. In conclusion, boar age is an important physiological factor contributing to the success of swine AI.

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Section: Discussionmentioning

confidence: 97%

Age-related changes in quality and fertility of porcine semen

Tsakmakidis

Khalifa²,

Boscos

2012

Biol. Res.

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“…In this sense Parrilla et al (2012) showed that ejaculate spermatozoa from individual boars can respond in a boar-dependent manner to different semen-processing techniques. It could be then hypothesized that different qualitative characteristics, such as DNA fragmentation index (Lopez-Fernandez et al 2008) of sperm samples, could be compromising the TX pre-treatment reproducibility within different boar samples and could be the basis of the different fertilization rates obtained from different males, since DNA fragmentation in boar sperm has been correlated with poor fertility outcomes (Boe-Hansen et al 2008;Didion et al 2009). Moreover, in further experiments it could be interesting to study the effect of the TX treatment in later embryo development stages.…”

Section: Discussionmentioning

confidence: 99%

Male pronucleus formation after ICSI: effect of oocyte cysteine or sperm Triton X-100 treatments

García-Mengual¹,

Silvestre²,

Salvador³

et al. 2015

CZECH J ANIM SCI

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ABSTRACT:In pigs, intracytoplasmic sperm injection (ICSI) efficiency is still poor. The inadequate decondensation of the sperm chromatin, its transformation into the male pronucleus (MPN) together with the subsequent inability to activate the oocyte, seem to be the main causes of the low ICSI efficiency. In order to improve the MPN formation we took two different approaches. On the one hand, the in vitro culture (IVC) medium post-ICSI was supplemented with 1.71mM cysteine (CYS). Alternatively, the sperm membrane was digested with Triton X-100 (TX) before ICSI, to improve the exposure of the sperm chromatin to the oocyte cytoplasm. After 6 h post-ICSI, the activation rate was significantly higher in TX group (70.0%) compared with CYS and control groups (42.2% and 48.9%, respectively; P < 0.05). However, no significant differences between the three groups were observed in terms of the number of pronuclei, 2PN (oocytes with 2 pronuclei and no visible sperm), and 1PN + sperm (oocytes with 1 pronucleus and one sperm head). At 22 h post-ICSI, the activation rates were similar in TX, CYS, and control groups (73.1, 78.9, and 75.7%, respectively). In addition, we did not observe significant differences between TX, CYS, and control groups for the number of pronuclei, 2PN (52.6, 56.7, and 50%, respectively) or 1PN + sperm (21.1, 33.3, and 32.1%, respectively). While no cleavage was observed in the CYS group, no significant differences in the cleavage rate were observed between control (21.3%) and TX (10.5%) groups. In summary, and under our conditions, neither CYS supplement, nor sperm TX pre-treatment were able to improve MPN formation at 6 and 22 h post-ICSI. However, the sperm TX pre-treatment improved oocyte activation at 6 h post-ICSI, although 22 h post-ICSI such a beneficial effect did not persist.Keywords: porcine; assisted reproductive technology; pronuclear formation List of abbreviations: ICSI = intracytoplasmic sperm injection, PN = pronucleus, MPN = male pronucleus, IVC = in vitro culture, CYS = cysteine, TX = Triton X-100, IVP = in vitro production, IVM = in vitro maturation, IVF = in vitro fertilization, EGF = epidermal growth factor, GSH = glutathione, COC = cumulus oocyte complex, PZM-3 = porcine zygote medium-3, MM199 = maturation medium, HM199 = handling medium 242

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“…They are however used for research on porcine sperm. DNA fragmentation tests can be used to identify subfertile boars, but the study results are contradictory (Waberski et al, 2011;Boe Hansen et al, 2008). Some metabolic responses of sperm like resistance to oxidative stress (López et al, 2010) and in vitro fertilisation assays are also used for research purposes.…”

Section: Other Sperm Examination Techniquesmentioning

confidence: 99%