Sperm distribution and fertilization after unilateral and bilateral laparoscopic artificial insemination with frozen-thawed goat semen

Anakkul, Nitira; Suwimonteerabutr, Junpen; Tharasanit, Theerawat; Khunmanee, Sarawanee; Diloksumpan, Paweena; Berg, D.K.; Techakumphu, Mongkol

doi:10.1016/j.theriogenology.2014.07.032

Cited by 12 publications

(9 citation statements)

References 34 publications

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“…No such data were available for goat semen doses. Anakkul et al (2014) found no significant differences in kidding rates when 60 x 10 6 or 120 x 10 6 spermatozoa were used in LAI in Saanen goats (50.33% vs. 60%, respectively).…”

Section: Discussionmentioning

confidence: 73%

“…This high dosage used in cervical or vaginal AI allows only 10-15 females to be inseminated with ejaculate from bucks or rams. In contrast, LAI warranties higher pregnancy rates using frozen-thawed/low doses (10-20x10 6 ) semen (Anakkul et al, 2014;Kulaksiz and Ari, 2016). In this way, LAI provides more efficient and widespread semen usage from an elite buck or ram for AI.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the determination of the optimum number of spermatozoa is required to achieve the highest pregnancy rates in goats. A wide range of insemination doses (5x10 6 to 200x10 6 sperm) has been reported for frozen-thawed goat spermatozoa, and these doses depend largely on the AI technique that is employed (Anakkul et al, 2014). Therefore, this study's major objective was to compare the kidding rates of goats laparoscopically inseminated by different sperm doses and to determine the minimal sperm dose per intrauterine insemination to maximize the genetic diffusion of superior Boer and Zaraiby males, without decreasing AI success.…”

Section: Introductionmentioning

confidence: 99%

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Clinical Application Of Intrauterine Laparoscopic Insemination In Zaraiby Goats Using Different Concentrations Of Zaraiby And Boer Bucks’ Frozen Semen

Leil

El-Shamy²,

D.A.³

2020

Journal of Applied Veterinary Sciences

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This study aimed to determine the proper sperm dose per laparoscopic intrauterine insemination (LAI) in Zaraiby goats. Thirty-six Zaraiby goats, two Zaraiby and two Boer bucks were used in the study. Does were divided into six groups; the first three groups were inseminated laparoscopically with frozen-thawed Zaraiby semen either at doses of 10 x 10 6 (GP1), 20 x 10 6 (GP 2), or 40 x 10 6 (GP 3). The other three groups were inseminated with Boer semen's same doses (GP 4, 5 &6). Results showed that goats laparoscopically inseminated with 20 x 10 6 Zaraiby spermatozoa or 10 x 10 6 Boer sperm cells both had the highest (P ≤0.05) pregnancy rate (about 70% & 60%, respectively). The multiple birth rate in goats inseminated by Boer buck's spermatozoa were generally higher than those inseminated with Zaraiby buck's spermatozoa (66.67 to 100.00% vs. 25.00 to 40.00%, respectively). In conclusion, the LAI is an efficient means of achieving high fertilization rates in goats. The recommended minimum necessary dose for laparoscopic artificial insemination in Zaraiby is 10 ×10 6 and 20×10 6 motile Boer and Zaraiby buck's spermatozoa, respectively.

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Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Clinical Application Of Intrauterine Laparoscopic Insemination In Zaraiby Goats Using Different Concentrations Of Zaraiby And Boer Bucks’ Frozen Semen

Leil

El-Shamy²,

D.A.³

2020

Journal of Applied Veterinary Sciences

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“…This difficulty in cervical passage of the inseminating pipette has been overcome by deposition of the semen directly into the uterus with the help of a laparoscope [16,17]. And higher fertilization rates have been reported in sheep after laparoscopic insemination (92.5%) compared to cervical AI (39.67%) [3,[18][19][20]. Although laparoscopic insemination can provide better fertility with F-T semen, it cannot be effectively used at a mass scale because of its limitations including higher cost and the technical expertise required to perform it.…”

Section: Introductionmentioning

confidence: 99%

Topical application of cervix with hyaluronan improves fertility in goats inseminated with frozen-thawed semen

Leethongdee

Thuangsanthia²,

Khalid

2021

Anim Biosci

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The artificial insemination play an important role in the genetic improvement in goat farming system. The aim of this study was to investigate the effect of cervical application of Hyaluronan (HA) on the fertility in goats after cervical artificial insemination (CAI) using frozen-thawed (F-T semen). Methods: After oestrous synchronisation with progesterone sponges and pregnant mare serum gonadotropin (PMSG) injection, both nulli-and multi-parous goats, were randomly allocated to 2 groups, and were inseminated with 0.25 ml of F-T semen (150 x 10 6 spermatozoa) twice at 52 h and 56 h after sponge removal. Prior to the insemination, goats in Group 1 only were given topical cervical HA application at 48 h after sponge removal. Site of insemination was recorded as os-cervix or intra-cervix or intra-uterus. Pregnancy was tested ultrasonographically 42 days after insemination. The data on pregnancy rates and percentage of animals according to the site of semen deposition were compared by Chi-square analysis. Results: The overall pregnancy rate was significantly (p<0.004) higher in goats with prior application to the cervix with HA (63.3%) than without (36.0%). Same pattern was observed in the pregnancy rates of nulli-and multi-parous goats in both the groups. Percentage of nulliparous goats according to the site of insemination in the HA group did not differ between first and the second insemination. However, in multiparous goats the percentage of animals inseminated intra-cervically was significantly increased (p≤ 0.05) between the first and the second inseminations. Conclusion: The results suggest that significantly higher fertility rate in the "HA goats" compared to the "without HA" group was because of deeper insemination facilitated by topical cervical application of HA. The deeper insemination into the cervical canal increase the rate of fertilisation when the cervical artificially insemination is performed.

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“…Laparoscopy has been used successfully in the reproductive management of small ruminants, mainly in AI where pregnancy rates from 60 to 75% have been reported (Anakkul et al., ; Dovenski et al., ; Martinez et al., ). Although there are some reports in which laparoscopy has been used to monitor the reproductive tract in cattle (Babkine & Desrochers, ), information regarding its use in AI is scarce.…”

Section: Introductionmentioning

confidence: 99%

A new device to inseminate cows at the base of the uterine horns

García-Peña

Rangel-Santos

Lara

et al. 2016

Reprod Domestic Animals

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A new device (Chapingo device) to deposit semen at the base of the uterine horns of cattle was developed at Universidad Autonoma Chapingo, Mexico. Nine Holstein heifers were inseminated by transvaginal laparoscopy, using a laparoscope for cattle and the Chapingo device. A dose of sexed semen (2.1 × 10 spermatozoa) was deposited at the base of the uterine horn ipsilateral to the ovary where the preovulatory follicle was identified. Insemination was achieved in all the heifers, taking on average 13.7 ± 3.1 min per animal. In all cases, it was possible to see both ovaries, the base of the uterine horns and the oviducts. After the procedure, none of the heifers showed any type of complications such as haemorrhage, adhesions or trauma. On days 21 and 22 after insemination, four of the nine heifers (44.4%) returned into oestrus; on day 30 after insemination, one heifer was found to be pregnant by ultrasound. The results show the feasibility of generating pregnancies by transvaginal laparoscopy in heifers inseminated with sexed semen.

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Sperm distribution and fertilization after unilateral and bilateral laparoscopic artificial insemination with frozen-thawed goat semen

Cited by 12 publications

References 34 publications

Clinical Application Of Intrauterine Laparoscopic Insemination In Zaraiby Goats Using Different Concentrations Of Zaraiby And Boer Bucks’ Frozen Semen

Clinical Application Of Intrauterine Laparoscopic Insemination In Zaraiby Goats Using Different Concentrations Of Zaraiby And Boer Bucks’ Frozen Semen

Topical application of cervix with hyaluronan improves fertility in goats inseminated with frozen-thawed semen

A new device to inseminate cows at the base of the uterine horns

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