Sperm DNA fragmentation and male fertility: a retrospective study of 5114 men attending a reproductive center

Zhang, Fengbin; Li, Jingping; Liang, Zhong-Yan; Wu, Jing-Gen; Li, Lejun; Chen, Chong; Jin, Fan; Tian, Yonghong

doi:10.1007/s10815-021-02120-5

Cited by 23 publications

(17 citation statements)

References 44 publications

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“…Also, as demonstrated in Figure 2, sperm DNA fragmentation was notably lower in those with normal semen characteristics than in those with abnormal semen characteristics. Our study was well correlated with the studies conducted by Choucair et al (2016) and Zhang et al (2021), which reported noticeably lower DFI in the NZ group compared to other infertile groups except for the OZ group (Choucair et al, 2016;Zhang et al, 2021). Our results demonstrated insignificant differences between NZ and OZ groups regarding sperm DFI.…”

Section: Discussionsupporting

confidence: 91%

Evaluation of sperm DNA fragmentation in male infertility

et al. 2022

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Sperm DNA integrity could be considered a biological marker of sperm quality and may affect fertilization, embryonic development, and pregnancy outcome. The study aimed to investigate the connection between semen characteristics and sperm DNA damage in infertile patients. Standard semen analysis of 536 samples was carried out following the World Health Organization (WHO) 5th edition recommendations. Sperm DNA damage was assessed by the terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling (TUNEL) assay, after preparation by direct swim‐up. The slides were evaluated using a fluorescence microscope and the percentage of TUNEL‐positive spermatozoa was expressed as the DNA fragmentation index (DFI). Patients were classified according to their DFI levels: group A (DFI < 15%) and group B (DFI ≥15%). Sperm total count, concentration, total and progressive motility, vitality, and normal morphology were significantly higher in group A compared to group B (p < 0.001). The results show a significant inverse correlation between DFI and patient's age, sperm total count, concentration, total and progressive motility, vitality and normal morphology. Higher DFI values were found to be strongly associated with poor sperm quality. In conclusion, combined with conventional semen analysis, assessment of sperm DFI could improve diagnostic accuracy and treatment management for patients with male infertility.

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Section: Discussionsupporting

confidence: 91%

Evaluation of sperm DNA fragmentation in male infertility

et al. 2022

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“…In the current study, the analysis of 1638 IVF/ICSI cycles showed that the sperm DFI was significantly positively correlated with male age, indicating that the degree of sperm DNA fragmentation increased with age (Supplementary Table 1). This is consistent with the findings of Bellver (15), Ghanbarzadeh (16), Zhang (17), Gonzalez (18), Lu (19) and Belloc (20). The sperm DFI was significantly and positively correlated with abstinence days, semen volume, immotile sperm percentage, sperm high-staining HDS percentage and other parameters, suggesting that the increase in abstinence days may increase semen volume, but the immotile sperm percentage and sperm high-staining HDS percentage may increase at the same time.…”

Section: Discussionsupporting

confidence: 93%

Influence of sperm DNA fragmentation on the clinical outcome of in vitro fertilization-embryo transfer (IVF-ET)

et al. 2022

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PurposeTo evaluate the effect of elevated sperm DNA fragmentation index (DFI) on fresh and frozen embryo transfer cycles.MethodsA retrospective study was performed with 549 fresh embryo transfer cycles and 1340 frozen embryo transfer cycles after in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) from 2016 to 2021.ResultsThe statistical results of 549 fresh embryo transfer cycles showed that the delivery rate in the normal sperm DFI group (43.9% vs. 27.1%, P = 0.014) was significantly higher than that in the abnormal sperm DFI group, and there were no significant differences in the biochemical pregnancy rate (59.0% vs. 50.8%, P = 0.232), clinical pregnancy rate (53.1% vs. 40.7%, P = 0.072), or miscarriage rate (17.3% vs. 33.3%, P = 0.098) between the two groups. The results of 1340 frozen embryo transfer cycles showed that the biochemical pregnancy rate (57.9% vs. 45.6%, P = 0.006) and clinical pregnancy rate (50.3% vs. 40.7%, P = 0.027) in the normal sperm DFI group were significantly higher than those in the abnormal sperm DFI group. The delivery rate (40.9% vs. 33.3%, P = 0.074) and miscarriage rate (18.6% vs. 18.0%, P = 0.919) were not significantly different between the two groups.ConclusionThe increase of sperm DFI significantly reduced the delivery rate of fresh embryo transfer cycles and the biochemical pregnancy rate and clinical pregnancy rate of frozen embryo transfer cycles.

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“…Damage to sperm chromatin can directly affect normal physiological functions and also leads to the transmission of incorrect genetic information to the offspring. Sperm DFI is a parameter that assesses male fertility 8 , 9 . Nowadays, sperm DFI has become an adjunct test for standard semen analysis.…”

Section: Introductionmentioning

confidence: 99%

“…The simultaneous detection of the above-mentioned semen parameters can provide a comprehensive understanding of the patient’s semen status, sperm motility, sperm morphology, defect degree of genetic material in sperm head nucleus, and accessory gonadal secretion function, which has important guiding significance for the diagnosis and treatment of infertility. Although SDF has been increasingly evaluated in the clinical setting, it is not yet recommended as a routine test by the American Society for Reproductive Medicine 9 . In contrast, the American Urological Association (AUA) and the European Association of Urology (EWAU) have acknowledged the importance of DNA fragmentation in sperm as guidelines on male infertility 10 .…”

Section: Introductionmentioning

confidence: 99%

Correlation analysis of sperm DNA fragmentation index with semen parameters and the effect of sperm DFI on outcomes of ART

Liu

Mao

Pan

et al. 2023

Sci Rep

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Routine semen analysis provides limited information about a man’s male reproductive potential and can not always fully explain male infertility. Many male infertilities are caused by sperm DNA defects that routine semen quality analyses fail to detect. In this study, we analyzed the association of sperm DNA fragmentation index (DFI) with the semen routine, sperm morphology, in vitro fertilization embryo transfer (IVF-ET)/intracytoplasmic sperm injection (ICSI). Further, we explored the predictive value of sperm DFI in evaluating male fertility and the outcome of IVF-ET/ICSI. Data on sperm DFI, sperm routine, and sperm morphology were collected from 1462 males with infertility. According to DFI levels, there were 468 cases in group I (DFI ≤ 15%), 518 cases in group II (15% < DFI < 30%), and 476 cases in group III (DFI ≥ 30%). The correlations of sperm DFI with semen routine and malformation rate were analyzed. Seminal plasma malondialdehyde (MDA), and total antioxidant capacity (TAC) were assessed. Sperm DFI, semen routine, and sperm morphology were detected in male patients of 101 pairs of IVF-ET/ICSI infertile couples and subdivided into IVF-I group (DFI ≤ 15%), IVF-II group (15% < DFI < 30%), IVF-III group (DFI ≥ 30%), ICSI-I group (DFI ≤ 15%), ICSI-II group (15% < DFI < 30%) and ICSI-III group (DFI ≥ 30%) according to DFI value. The effect of sperm DFI on the outcome of IVF-ET/ICSI was analyzed. There were significant differences in sperm survival rate, sperm concentration, and PR% between groupIII and group II (P < 0.01). There were significant differences in sperm survival rate, sperm concentration and PR% between group III and group I (P < 0.01). There was no significant difference in semen volume, age, abstinence days, or percentage of normal sperm between the three groups (P > 0.05). DFI was positively correlated with MDA content ( P < 0.01) and negatively correlated with TAC (P < 0.01). Sperm DFI was negatively correlated with sperm survival rate, sperm concentration, and PR% (P < 0.01). There was no correlation with age, abstinence days, semen volume, or percentage of normal-form sperm (r = 0.16, 0.05, 0.04, -0.18, p > 0.05). Compared with IVF-I group, the sperm concentration and PR were decreased in IVF-III group. The sperm malformation rate was higher in IVF-III group than that in IVF-II group. Comparatively, the PR was decreased in ICSI-III group. The sperm malformation rate was higher in ICSI-III group than that of the ICSI-I group (P < 0.05). There were no statistically significant differences in fertilization rate, cleavage rate, embryo rate, and clinical pregnancy rate between IVF group or ICSI group, and between all subgroups (P > 0.05). Sperm DFI is negatively associated with sperm survival rate, sperm concentration, and PR%. Antioxidants can decrease the rate of DNA fragmentation. Sperm DFI has proven to be very valuable in the male fertility evaluation, but its significance as a predictor of pregnancy outcomes following assisted reproductive technology. (ART) requires further investigation.

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Sperm DNA fragmentation and male fertility: a retrospective study of 5114 men attending a reproductive center

Cited by 23 publications

References 44 publications

Evaluation of sperm DNA fragmentation in male infertility

Evaluation of sperm DNA fragmentation in male infertility

Influence of sperm DNA fragmentation on the clinical outcome of in vitro fertilization-embryo transfer (IVF-ET)

Correlation analysis of sperm DNA fragmentation index with semen parameters and the effect of sperm DFI on outcomes of ART

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