Sperm DNA fragmentation in cryopreserved samples from subjects with different cancers

Cambi, Marta; Marchiani, Sara; Manigrasso, Ilaria; Degl'Innocenti, S; Forti, Gianni; Maggi, Mario; Baldi, Elisabetta; Muratori, Monica

doi:10.1071/rd15190

Cited by 14 publications

(9 citation statements)

References 53 publications

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“…In addition to the negative impact of the disease itself or oncological gonadotoxic treatment on the basic criteria of semen quality (number, motility, morphology), the negative impact of the disease is also reflected in increased sperm DNA fragmentation. 25 , 26 Meta-analyses have shown that increased sperm DNA fragmentation negatively affects IVF/ICSI outcomes even in the healthy male population. 27 , 28 …”

Section: Discussionmentioning

confidence: 99%

The outcome of IVF/ICSI cycles in male cancer patients: retrospective analysis of procedures from 2004 to 2018

Papler

Vrtačnik-Bokal

Drobnič

et al. 2021

Radiology and Oncology

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IntroductionFertility preservation is an important aspect of quality of life in oncological patients, and in men is achieved by semen cryopreservation prior to treatment. Results of in vitro fertilization (IVF) procedures in healthy infertile couples are comparable, regardless of whether fresh or cryopreserved semen is used, but are scarce in male oncological patients.Patients and methodsWe performed a retrospective analysis of IVF/intracytoplasmic sperm injection (IVF/ICSI) procedures in infertile couples where men had been treated for cancer in the past. We additionally compared the results of IVF/ICSI procedures with respect to the type of semen used (fresh, cryopreserved).ResultsWe compared the success rates of 214 IVF/ICSI cycles performed in the years 2004–2018. Pregnancy (30.0% vs. 21.4%; p = 0.12) and live-birth rates (22.3% vs. 17.9%; p = 0.43) per oocyte aspiration were similar between the groups in fresh cycles; however embryo utilization (48.9% vs. 40.0%; p = 0.006) and embryo cryopreservation rates (17.3% vs. 12.7%; p = 0.048) were significantly higher in the cryopreserved semen group. The cumulative pregnancy rate (60.6% vs. 37.7%; p = 0.012) was significantly higher, and the live-birth rate (45.1% vs. 34.0%; p = 0.21) non-significantly higher, in the cryopreserved semen group.ConclusionsThe success of IVF/ICSI procedures in couples where the male partner was treated for cancer in the past are the same in terms of pregnancies and live-births in fresh cycles regardless of the type of semen used. However, embryo utilization and embryo cryopreservation rates are significantly higher when cryopreserved semen is used, leading to a significantly higher cumulative number of couples who achieved at least one pregnancy.

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Section: Discussionmentioning

confidence: 99%

The outcome of IVF/ICSI cycles in male cancer patients: retrospective analysis of procedures from 2004 to 2018

Papler

Vrtačnik-Bokal

Drobnič

et al. 2021

Radiology and Oncology

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“…Indeed, in a previous study from our laboratory, we demonstrated that spermatozoa from oncological patients have higher DNA damage after thawing in respect to non-cancer patients, implying a higher susceptibility to thermic shock in oncological patients. 36 In our current study, in order to obtain information on the genomic integrity of spermatozoa in the context of natural conception, we analyzed fresh semen samples at all time-points.…”

Section: Discussionmentioning

confidence: 99%

“…However, concerns were raised about the genetic integrity of spermatozoa after genotoxic treatments, especially based on the observations about the persistence of increased aneuploidy rate and DNA fragmentation in some patients up to 18/24 months 9–13 . It is well known that etoposide, a component of the BEP regimen, inhibits both topoisomerase II activity and the disjunction of recombinant chromosomes and sister chromatids, leading to aneuploidy 35,36 . On the other hand, platinum‐based drugs, that is, cisplatin and CP, and RT are known to cause DNA strand breaks.…”

Section: Discussionmentioning

confidence: 99%

Long‐term effect of cytotoxic treatments on sperm DNA fragmentation in patients affected by testicular germ cell tumor

et al. 2023

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IntroductionTesticular germ cell tumor is the most frequent neoplasia in men of reproductive age, with a 5‐year survival rate of 95%. Antineoplastic treatments induce sperm DNA fragmentation, especially within the first year post‐therapy. Data in the literature are heterogeneous concerning longer follow‐up periods, and the large majority is limited to 2 years.ObjectiveTo define the timing for the recovery of sperm DNA damage and the proportion of patients with severe DNA damage at 2 and 3 years from the end of therapy.Materials and methodsSperm DNA fragmentation was evaluated in 115 testicular germ cell tumor patients using terminal deoxynucleotidyl transferase dUTP nick end labeling assay coupled with flow cytometry before (T0) and 2 (T2) and 3 (T3) years post‐treatment. Patients were divided based on the type of treatment: carboplatin, bleomycin–etoposide–cisplatin, and radiotherapy. For 24 patients, paired sperm DNA fragmentation data were available at all time‐points (T0–T2–T3). Seventy‐nine cancer‐free, fertile normozoospermic men served as controls. Severe DNA damage was defined as the 95th percentile in controls (sperm DNA fragmentation = 50%).ResultsComparing patients versus controls, we observed: (i) no differences at T0 and T3 and (ii) significantly higher sperm DNA fragmentation levels (p < 0.05) at T2 in all treatment groups. Comparing pre‐ and post‐therapy in the 115 patients, the median sperm DNA fragmentation values were higher in all groups at T2, reaching significance (p < 0.05) only in the carboplatin group. While the median sperm DNA fragmentation values were also higher in the strictly paired cohort at T2, about 50% of patients returned to baseline. The proportion of severe DNA damage in the entire cohort was 23.4% and 4.8% of patients at T2 and T3, respectively.DiscussionCurrently, testicular germ cell tumor patients are advised to wait 2 years post‐therapy before seeking natural pregnancy. Our results suggest that this period may not be sufficient for all patients.ConclusionThe analysis of sperm DNA fragmentation may represent a useful biomarker for pre‐conception counseling following cancer treatment.

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“…Fluorescence in situ hybridization (FISH) analysis may be used to determine the proportion of sperm with aneuploidy [ 67 ]. Novel methods of assessing fertility in male patients with cancer include assessing sperm deoxyribonucleic acid (DNA) methylation patterns [ 68 ] and levels of sperm DNA fragmentation in thawed samples [ 69 ]. Anti-Müllerian hormone (AMH) has been demonstrated as a surrogate marker for testicular damage in mice studies [ 70 ].…”

Section: Sperm Banking For Cancermentioning

confidence: 99%

Process and Pitfalls of Sperm Cryopreservation

Rozati

Handley

Jayasena

2017

JCM

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Sperm cryopreservation has been utilized routinely for over 40 years to preserve fertility in men undergoing cancer therapy and allow conception for infertile couples. This article provides a concise and up-to-date review of the literature and covers the latest advances in sperm cryopreservation and its array of clinical indications. Over recent years, the scope of clinical indications used for sperm cryopreservation has expanded widely. Consequently, more patient groups are eligible for sperm freezing, requiring specialist resources and higher running costs. Although sperm cryopreservation prior to cancer therapy is readily available in many countries, referral rates by oncology specialists and levels of patient engagement with cryopreservation services are both reported as low. Furthermore, sperm banking continues to raise ethical issues such whether sperm donation should be anonymous and whether sperm can be utilized posthumously by the surviving partner without consent from the patient. This review focuses on the technological advances and ethical controversies in sperm cryopreservation, and how better understanding of these issues could lead to improved access to fertility preserving treatment for patients.

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Sperm DNA fragmentation in cryopreserved samples from subjects with different cancers

Cited by 14 publications

References 53 publications

The outcome of IVF/ICSI cycles in male cancer patients: retrospective analysis of procedures from 2004 to 2018

The outcome of IVF/ICSI cycles in male cancer patients: retrospective analysis of procedures from 2004 to 2018

Long‐term effect of cytotoxic treatments on sperm DNA fragmentation in patients affected by testicular germ cell tumor

Process and Pitfalls of Sperm Cryopreservation

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