Sperm-Egg Interaction: Evidence for Boar Sperm Plasma Membrane Receptors for Porcine Zona Pellucida

Peterson, R. N.; Russell, Lonnie D.; Bundman, Donna; Freund, M.

doi:10.1126/science.7188647

Cited by 96 publications

(27 citation statements)

References 11 publications

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“…However, it was clearly shown in the present study that incubation of pig spermatozoa in the presence of DFM-H, Swainsonine or galactal, the inhibitors of α-Lfucosidase, α-D-mannosidase and β-Dgalactosidase, respectively, did not affect sperm motility but greatly reduced the number of spermatozoa bound to the ZP. Although it has been reported that pig spermatozoa which initially bind to the ZP have intact plasma membrane [30][31][32], the present results indicate that, in the pig, only spermatozoa undergoing or completing acrosome reaction could bind firmly to the ZP as suggested by Jones et al [33]. However, it is unclear at present how α-L-fucosidase might be involved in binding of spermatozoa to the ZP because it has been shown that fucose residues are present only in the inner region of the ZP [23].…”

Section: Discussionmentioning

confidence: 99%

Identification, Localization and Involvement of Glycosidases in Sperm-Zona Interaction Using Frozen-Thawed Ejaculated Pig Spermatozoa.

Song

Lyu

Park

et al. 2000

Journal of Reproduction and Development

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Abstract. To identify sperm glycosidases specific to sugar residues found in the zona pellucida of pig oocytes, frozen-thawed ejaculated spermatozoa were treated experimentally and assayed for activities of α-L-fucosidase, α-D-mannosidase, β-D-galactosidase and N-acetyl-β-D-glucosaminidase (GlcNAc'ase). When spermatozoa were incubated with calcium ionophore A23187 for 1 h, about 70% were acrosome-reacted and about 72, 46, 56 and 35% of the activities of α-L-fucosidase, α-Dmannosidase, β-D-galactosidase and GlcNAc'ase, respectively, were lost from spermatozoa. However, lower levels of α-L-fucosidase, α-D-mannosidase and β-D-galactosidase activity, and a higher level of GlcNAc'ase activity were released from spermatozoa treated with protease. Furthermore, treating spermatozoa with the detergent n-octylglucoside caused the release of similar amounts of α-L-fucosidase and β-D-galactosidase activity and higher amounts of α-D-mannosidase and GlcNAc'ase activity, compared with those treated with calcium ionophore. Protease and noctylglucoside treatments released similar amounts GlcNAc'ase activity. These results suggest that GlcNAc'ase is present mainly in the plasma membrane of pig spermatozoa, but that α-L-fucosidase, α-D-mannosidase and β-D-galactosidase are mainly found in the acrosome matrix including the outer acrosomal membrane, and that α-D-mannosidase may also be located in the inner acrosomal membrane. Maximal activity of α-D-mannosidase was at around physiological pH (7-8), but the other three glycosidases showed maximal activity at pH 4-5. When cumulus-free pig oocytes matured in culture were inseminated in the presence of various glycosidase inhibitors, the number of spermatozoa bound to the zona pellucida and the proportion of oocytes penetrated were greatly reduced by the inhibitors of α-L-fucosidase, α-D-mannosidase and β-D-galactosidase, but not by an inhibitor of GlcNAc'ase. These results indicate that α-L-fucosidase, α-D-mannosidase and β-Dgalactosidase are all important glycosidases involved in pig sperm-zona interaction.

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Section: Discussionmentioning

confidence: 99%

Identification, Localization and Involvement of Glycosidases in Sperm-Zona Interaction Using Frozen-Thawed Ejaculated Pig Spermatozoa.

Song

Lyu

Park

et al. 2000

Journal of Reproduction and Development

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“…Current evidence suggests that species specificity in gamete interaction is determined by components on the surface of the sperm plasma membrane and that of the zona pellucida (Peterson, Russell, Bundman & Freund, 1979, 1980. The membrane components involved in gamete recognition have been identified in several species.…”

Section: Introductionmentioning

confidence: 99%

“…Ligand-like elements were demonstrated on boar spermatozoa by the use of antiserum and univalent (Fab) antibody (Peterson et al, 1979(Peterson et al, ,1980. Sugar treatment of mouse (Chapman, Lambert & Le, 1982 ; and hamster (Ahuja, 1982) (West, Freís, Papaioannous, Karr & Chapman, 1977).…”

Section: Introductionmentioning

confidence: 99%

Role of sperm-surface glycoproteins in gamete recognition in two mouse species

Lambert¹

1984

Reproduction

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“…However, this assumes that prematurely bound sperm are engaged in a productive binding-that is, an interaction which leads to penetration. Such an assumption is not necessarily justified because a number of sperm-zona binding reactions shown by ourselves and others do not necessarily lead to penetration (6,20).…”

Section: Discussionmentioning

confidence: 97%

“…The mammalian egg consists of an egg cell proper (or vitellus) surrounded by the zona pellucida, an acellular envelope composed largely of glycoproteins (1-4). The zona possesses species-specific binding sites for sperm (5,6) and is a barrier to multiple sperm penetration in most of the species studied (7,8). In both the hamster and the mouse, it has recently been shown that the sperm bind to the surface of the zona pellucida by means of the plasma membrane of the sperm head (9)(10)(11).…”

mentioning

confidence: 99%

Modulation of fertilization in vitro by peptides released during hamster sperm-zona pellucida interaction.

Hartmann¹,

Hutchison²

1981

Proc. Natl. Acad. Sci. U.S.A.

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The penetration of the zona pellucida by hamster sperm in vitro (assay dro s) was inhibited by aliquots of ultrafiltrates obtained from another set of drops (experimental drops) in which capacitated sperm had interacted with the surface of the zona pellucida. This inhibition was inversely related to the concentration of sperm in the assay drop, and it was noted that the inhibitory response declined when the gametes in the assay drop were incubated together beyond the standard 90-min period. Thus, inhibition was not irreversible. A number of criteria suggest that the inhibitory activity resides in at least some of the peptides (SI factors) released at four different times after contact between the surfaces of the sperm and zona pellucida. Like the SI factors, the substances responsible for the inhibitory activity of the supernatants, collected at 2 and 50 min after gamete combination, traversed ultrafilters with Mr cutoffs of 5000 and 2000, respectively, and were inactivated by subtilisin. In addition, the quantity of inhibitory material recovered in the ultrafiltrates from experimental drops containing various numbers of eggs was well correlated with the amount of 2-and 50-min SI factors from similar experimental drops. These results suggest that the S1 factors are peptide modulators of the penetration process and it is speculated that one of their functions may be to prevent or at least to discourage polyspermic penetration of the hamster zona pellucida.After initial contact between gametes is made, fertilization of the mammalian egg involves a sequence of events that presumably operate in concert to ensure monospermic penetration. Precisely how this occurs is not known, but it is reasonable to assume that nature has devised subtle control mechanisms to effect this phenomenon. In general, what is known about these early steps is based largely on observations made with in vitro systems, a summary of which follows. The mammalian egg consists of an egg cell proper (or vitellus) surrounded by the zona pellucida, an acellular envelope composed largely of glycoproteins (1-4). The zona possesses species-specific binding sites for sperm (5, 6) and is a barrier to multiple sperm penetration in most of the species studied (7,8). In both the hamster and the mouse, it has recently been shown that the sperm bind to the surface of the zona pellucida by means of the plasma membrane of the sperm head (9-11). In these species it has also been shown that the acrosome reaction, a drastic alteration in the integrity of the plasma membrane of the sperm head and subadjacent outer acrosomal membrane that is a necessary prelude to penetration of the zona pellucida, occurs while sperm are associated with its surface (9, 10). Once the zona has been penetrated by a sperm, contact with the surface of the vitellus triggers exocytosis of the cortical granules situated subadjacent to the vitelline membrane. The expelled contents of the granules alter the zona, which results in the inhibition of penetration by additional sperm (7,8,1...

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Sperm-Egg Interaction: Evidence for Boar Sperm Plasma Membrane Receptors for Porcine Zona Pellucida

Cited by 96 publications

References 11 publications

Identification, Localization and Involvement of Glycosidases in Sperm-Zona Interaction Using Frozen-Thawed Ejaculated Pig Spermatozoa.

Identification, Localization and Involvement of Glycosidases in Sperm-Zona Interaction Using Frozen-Thawed Ejaculated Pig Spermatozoa.

Role of sperm-surface glycoproteins in gamete recognition in two mouse species

Modulation of fertilization in vitro by peptides released during hamster sperm-zona pellucida interaction.

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