Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse

Fujihara, Yoshitaka; Murakami, Masao; Inoue, Naokazu; Satouh, Yuhkoh; Kaseda, Kazuhiro; Ikawa, Masahito; Okabe, Masaru

doi:10.1242/jcs.067363

Cited by 92 publications

(96 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…59 A mouse line lacking Spesp1 was produced by homologous recombination and the fertilizing ability of male mice was examined. 60 The decreased fertilizing ability was found not only in Spesp1 2/2 sperm but also in Spesp1 1/2 sperm. Despite the normal number of ejaculated sperm and normal motility, Spesp1-disrupted mouse had a decreased number of pups compared to wild-type mice.…”

Section: Gamete Interaction N Inoue Et Al 84mentioning

confidence: 96%

The mechanism of sperm–egg interaction and the involvement of IZUMO1 in fusion

Inoue¹,

Ikawa²,

Okabe³

2010

Asian J Androl

Self Cite

View full text Add to dashboard Cite

An average human ejaculate contains over 100 million sperm, but only a few succeed in accomplishing the journey to an egg by migration through the female reproductive tract. Among these few sperm, only one participates in fertilization. There might be an ingenious molecular mechanism to ensure that the very best sperm fertilize an egg. However, recent gene disruption experiments in mice have revealed that many factors previously described as important for fertilization are largely dispensable. One could argue that the fertilization mechanism is made robust against gene disruptions. However, this is not likely, as there are already six different gene-disrupted mouse lines (Calmegin, Adam1a, Adam2, Adam3, Ace and Pgap1), all of which result in male sterility. The sperm from these animals are known to have defective zona-binding ability and at the same time lose oviduct-migrating ability. Concerning spermzona binding, the widely accepted involvement of sugar moiety on zona pellucida 3 (ZP3) is indicated to be dispensable by gene disruption experiments. Thus, the landscape of the mechanism of fertilization is revolving considerably. In the sperm-egg fusion process, CD9 on egg and IZUMO1 on sperm have emerged as essential factors. This review focuses on the mechanism of fertilization elucidated by gene-manipulated animals.

show abstract

Section: Gamete Interaction N Inoue Et Al 84mentioning

confidence: 96%

The mechanism of sperm–egg interaction and the involvement of IZUMO1 in fusion

Inoue¹,

Ikawa²,

Okabe³

2010

Asian J Androl

Self Cite

View full text Add to dashboard Cite

show abstract

“…Note: It is unclear if CD9 and/or different tetraspanins function in other species' oocytes in gamete membrane fusion [76]. Other mouse knockouts have less severe defects in gamete interactions or fertilization, or have multiple gamete function defects (e.g., [77]). The discovery of CD9's role in murine fertilization occurred rather serendipitously, when the knockout mouse lacking this member of the protein family was found to have greatly reduced female fertility.…”

Section: Gamete Membrane Fusion and The Oocyte-to-embryo Transitionmentioning

confidence: 99%

“…Thus, proteins in the equatorial segment of the sperm head have been of interest. SPESP1 (sperm equatorial segment protein 1; [132]) is a candidate, based on the finding that Spesp1 -/-males produce slightly smaller litters than wild type controls (22%), and have sperm with reduced (although not completely deficient) ability to undergo sperm-oocyte fusion [77]. Other proteins associated with sperm-oocyte fusion and exposure or rearrangement after acrosome exocytosis include equatorin [133,134], Sperm Acrosomal Membrane-Associated the phenotype of the knockout [82].…”

Section: Gamete Membrane Fusion and The Oocyte-to-embryo Transitionmentioning

confidence: 99%

Membrane Fusions During Mammalian Fertilization

Gadella

Evans

2011

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Successful completion of fertilization in mammals requires three different types of membrane fusion events. Firstly, the sperm cell will need to secrete its acrosome contents (acrosome exocytosis; also known as the acrosome reaction); this allows the sperm to penetrate the extracellular matrix of the oocyte (zona pellucida) and to reach the oocyte plasma membrane, the site of fertilization. Next the sperm cell will bind and fuse with the oocyte plasma membrane (also known as the oolemma), which is a different type of fusion in which two different cells fuse together. Finally, the fertilized oocyte needs to prevent polyspermic fertilization, or fertilization by more than one sperm. To this end, the oocyte secretes the contents of cortical granules by exocytotic fusions of these vesicles with the oocyte plasma membrane over the entire oocyte cell surface (also known as the cortical reaction or cortical granule exocytosis). The secreted cortical contents modify the zona pellucida, converting it to a state that is unreceptive to sperm, constituting a block to polyspermy. In addition, there is a block at the level of the oolemma (also known as the membrane block to polyspermy). IntroductionFertilization of the oocyte involves three membrane fusion events [1] namely, (1) a preparative series of secretion membrane fusions at the apical sperm surface known as acrosome exocytosis [2]. The membrane fusions are induced when the sperm cell binds to specific zona binding proteins at the sperm surface [3][4][5][6][7]. The acrosome exocytosis is a multipoint membrane fusion event between the sperm plasma membrane and the outer acrosomal membrane (see Fig. 5.1 [8,9]) and the exposed acrosomal content is required for sperm to penetrate the zona pellucida [10][11][12]. This so-called zona drilling effectively takes place because the sperm at this stage also has acquired hyperactivated motility [13]. (2) After zona penetration the sperm enters the perivitelline space where it can bind and fuse with the oocyte plasma membrane [14,15]. This is the actual fertilization fusion in which the contents of the sperm are delivered into the oocyte cytoplasm. The plasma membrane of the equatorial segment (see Fig. 5.1) is the site where proteins are located that orchestrate sperm-oocyte binding and fusion [16]. (3) In order to prevent polyspermy the oocyte has to activate defense systems to block redundant sperm-oocyte fusion [17]. To this end the first fertilizing sperm delivers activation factors

show abstract

“…It is thought that ESP plays a role in adhesion of the spermatozoon to the oocyte and their fusion at the oolemma level. Fujihara et al [38] identified sperm equatorial segment protein 1 (SPESP1), which is specific to the equatorial segment.…”

Section: Acrosome and Perinuclear Thecamentioning

confidence: 99%

Ultrastructure of Spermatozoa from Infertility Patients

Брагина¹,

Bocharova²

2018

Spermatozoa - Facts and Perspectives

View full text Add to dashboard Cite

Standard examination of human semen currently remains a main test for examination of male fertility disorders. Although parameters of sperm quality in fertile men are generally higher than in sterile ones, there is a substantial overlap between the two populations, indicating that other important factors affect fertility, but are not assessed in conventional assay. Currently, tests determining the functional properties of sperm have been intensively developed. This review considers an electron microscopic examination of sperm, which assesses the structure and function of the sperm nuclear, penetration and motor apparatus. The detection of sperm chromatin structure can help to understand the causes of early embryonic malformation. Genetically caused and functional disorders of the structure and function of spermatozoa are discussed. Indications for electron microscopic examination of spermatozoa in fertility disorders are given.

show abstract

Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse

Cited by 92 publications

References 30 publications

The mechanism of sperm–egg interaction and the involvement of IZUMO1 in fusion

The mechanism of sperm–egg interaction and the involvement of IZUMO1 in fusion

Membrane Fusions During Mammalian Fertilization

Ultrastructure of Spermatozoa from Infertility Patients

Contact Info

Product

Resources

About