Sperm evaluation and morphological description of male genitalia of meerkats (Suricata suricatta)

Silvatti, Bruna; Granato, Thaís Marques; Jorge‐Neto, Pedro Nacib; Luppi, Marta Maria Círchia Pinto; Reisfeld, Laura; Henrique, Paloma Canedo; Padilha, Fabiana Lúcia André; Leite, Roberta Ferreira; Losano, João Diego de Agostini; Kawai, Giulia Kiyomi Vechiato; Nichi, Marcílio; Pizzutto, Cristiane Schilbach

doi:10.1016/j.anireprosci.2020.106585

Cited by 8 publications

(4 citation statements)

References 44 publications

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Section: Discussionmentioning

confidence: 99%

“…The electroejaculation technique allowed successful semen collection from 9 of 13 animals, however these results were less efficient in seminal quality (motility and vigor) when compared with Luba et al (2015) or with Silva, Araujo, et al (2019), where the anesthetic protocol included an alpha 2‐adrenergic agonist–xylazine and medetomidine, respectively. Alpha 2‐adrenergic agonists are used as facilitators of spermatic flow in anesthetic protocols for electroejaculation in other species (de Araujo et al, 2015; Deco‐Souza et al, 2013; Silvatti et al, 2020; Zambelli et al, 2007). Only seminal fluid was collected from two animals — that does not mean failure of the technique as perhaps these males had previously copulated as described in free‐living jaguar (Jorge‐Neto et al, 2020), and MtSo5 had previously mated and MtGi6 had later mated with females with there being offspring produced as a result — while no fluid was recovered from another two males (MtBu2 and MtIu7) without successful offspring produced as a result of previous mating (Table 1).…”

Section: Discussionmentioning

confidence: 99%

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Spermatic profile of captive giant anteaters (Myrmecophaga tridactyla): Knowing more to preserve better

et al. 2021

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The giant anteater (Myrmecophaga tridactyla) is being threatened by natural habitat destruction and fragmentation, illegal hunting and road kills. In this context, the generation of basic information on the reproductive parameters of this species is vital, aiming to improve reproductive management via, amongst others, assisted reproductive technologies. This study aimed to describe the morphological and functional features of semen collected from captive giant anteaters. Electroejaculation was performed in 13 animals housed in zoos located in São Paulo state, Brazil. Semen samples were collected from 13 animals in 16 procedures. Samples were evaluated for volume, motility, vigor, pH, concentration, sperm morphology, and functional tests. The following mean values were obtained: volume 1.28 ± 0.27 mL; motility 28.3 ± 6.2%; vigor 2.4 ± 0.25; concentration 129.4 ± 36.1 × 106 sperm/mL; pH 7.4 ± 0.2. Total acrosome, head, midpiece, and tail sperm abnormalities were 3.2 ± 0.8%, 25.4 ± 3.6%, 20.7 ± 3.2%, and 14.7 ± 2.6%, respectively. Intact acrosome was found in 83.7 ± 3.1% and intact membrane in 81.1 ± 4.0% of all samples collected. Mitochondrial activity was 66.4 ± 6.0% (Class I), 18.7 ± 2.9% (Class II), 8.0 ± 2.0% (Class III), 3.9 ± 1.0% (Class IV), and 3.0 ± 0.9% (Class V). Sperm DNA fragmentation rate was 13.2 ± 3.7%. These results indicated that electroejaculation is a feasible method for semen collection in giant anteaters, allowing a more detailed description of the semen in this species.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Spermatic profile of captive giant anteaters (Myrmecophaga tridactyla): Knowing more to preserve better

et al. 2021

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“…To the best of our knowledge, the present study was the first in which detomidine was used for feline semen collection thru urethral catheterization. Medetomidine, however, is widely used for this purpose as it is known to promote the release of sperm into the urethra, allowing the semen to be collected by urethral catheterization (Araujo et al, 2020b;Araujo et al, 2018;Jorge-Neto et al, 2020b;Jorge Neto et al, 2019;Lueders et al, 2012;Prochowska et al, 2015;Silva et al, 2019;Silvatti et al, 2020;Zambelli et al, 2008). Thereby, this study aimed to assess whether detomidine had the same effect as medetomidine for semen collection in domestic cats.…”

Section: Discussionmentioning

confidence: 99%

Can detomidine replace medetomidine for pharmacological semen collection in domestic cats?

et al. 2021

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Among the different methods used for semen collection from domestic cats, the pharmacological collection by urethral catheterization becomes disruptive. Medetomidine is the elected α 2 -adrenoceptor agonist for that, but in several countries, it is not commercially available. This study aimed to evaluate the efficacy of detomidine compared to medetomidine in collecting semen by urethral catheterization in domestic cats. Urethral catheterization was performed on 13 mongrel cats using a disposable semi-rigid tomcat urinary catheter. Of the 19 semen collections performed with medetomidine induction, 94.7% were successful, while with detomidine induction, only 56.3% of 16 were successful. The values semen samples variables were as follows for volume -10.56 ± 0.4 vs 8.88 ± 0.5 mL, motility -171.67 ± 0.79 vs 49.77 ± 3.45%, vigor -4.1 ± 0.03 vs 3.10 ± 0.1 and concentration -3.24 ± 0.19 vs 2.15 ± 0.13 ×10 9 sperm/mL respectively for medetomidine and detomidine group. The failure in semen collections with detomidine was mainly due to azoospermic samples, poor urethral relaxation, insufficient volume, or contamination of urine. The sperm concentration was also lower in the detomidine group (P <0.05) when compared to medetomidine. However, when the volume of semen collected was compared, we found no statistical differences. Despite its low performance in collecting semen from cats, detomidine may be an alternative when medetomidine is not accessible.

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“…The testicular volume was measured using a caliper and applying the equation for an ellipsoid 4/3 × π × L/2 × B1/2 × B2/2, where L, B1, and B2 are the length and two breadths of the ellipsoid ( Harcourt et al, 1995 ), then subsequently converted to grams (1.046) ( Johnson et al, 1981 ). The relative testicular weight (RTW) was determined by dividing the total testes' weight by the body weight ( Jorge-Neto et al, 2020c ; Silva et al, 2022 ; Silvatti et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Cryopreservation of jaguar (Panthera onca) sperm cells using different cryoprotectants and different thawing temperatures

et al. 2023

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The cryopreservation of jaguar semen must be improved to produce high-quality biobanking doses. Until now, the rare studies of semen freezing in the species have only evaluated glycerol, always with a significant reduction in sperm quality in thawed semen. The purpose of this study was to assess the efficacy of three cryoprotectants, dimethylsulfoxide (DMSO), glycerol (GLY), and methanol (MET), in the cryopreservation of jaguar semen in an LDL-based extender, as well as the effect of thawing temperature on dosage quality. Five mature males with a history of reproduction were used. On the males, an infrared thermal image (IRT) was captured, the spicules and testes were analyzed, and the CASA system was used to evaluate the quality of fresh and thawed sperm. The superficial IRT was 4.6 ± 1.2 °C cooler than the anal sphincter, and the semen measured between 27.3 and 28.7 °C shortly after exiting the urethra. The total motility of fresh sperm was 55.3 ± 22.6%, and progressive motility was 36.3 ± 18%. The total motility of thawed sperm was 5.28 ± 2.51%, 4.49 ± %2.49, and 0.51 ± 0.62% for DMSO, GLY, and MET, respectively. DMSO and GLY performed better than MET, and there was no difference in thawing temperature (37°C 30 s vs. 50°C 12 s). All animals exhibit a considerable level of morphological changes in sperm. Low amounts of total and progressive motility were found in the thawed sperm. Males with a high level of sperm morphological changes were found to be fertile, but the lone male with normospermia was infertile. Thus, we contest the applicability of the commonly used morphological classification for bovines to felid species.

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Sperm evaluation and morphological description of male genitalia of meerkats (Suricata suricatta)

Cited by 8 publications

References 44 publications

Spermatic profile of captive giant anteaters (Myrmecophaga tridactyla): Knowing more to preserve better

Spermatic profile of captive giant anteaters (Myrmecophaga tridactyla): Knowing more to preserve better

Can detomidine replace medetomidine for pharmacological semen collection in domestic cats?

Cryopreservation of jaguar (Panthera onca) sperm cells using different cryoprotectants and different thawing temperatures

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