Sperm factors related to failure of human in-vitro fertilization

Jeulin, C.; Feneux, D.; Serres, Catherine; Jouannet, Pierre; Guillet-Rosso, F.; Belaisch-Allart, J.; Frydman, René; Testart, J

doi:10.1530/jrf.0.0760735

Cited by 202 publications

(71 citation statements)

References 17 publications

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“…Sperm from infertility patients often exhibit anomalies related to the protamine content and composition (Balhorn et al, 1988;Foresta et al, 1992;Belokopytova et al, 1993;de Yebra et al, 1993;Carrell and Liu, 2001;Nasr-Esfahani et al, 2004;Aoki et al, 2005;Oliva, 2006;Torregrosa et al, in press). In addition, an increased ratio between histones and protamines has been reported to exist in the sperm of infertile men (Silvestroni et al, 1976;Terquem and Dadoune, 1983;Juelin et al, 1986;Chevaillier et al, 1987;Auger et al, 1990;Bach et al, 1990Blanchard et al, 1990Zhang et al, 2006). Overall, many of these changes in the basic nuclear protein complement, which presumably make the sperm chromatin more vulnerable, have been shown to correlate with the various nuclear and chromatin disturbances that are manifested in the sperm of many infertile men (Agarwal and Said, 2003).…”

Section: Discussionmentioning

confidence: 99%

Testis/sperm-specific histone 2B in the sperm of donors and subfertile patients: variability and relation to chromatin packaging

et al. 2006

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BACKGROUND: The compaction of human sperm chromatin is the result of replacement of ~85% of histones with protamines. Germ-line testis/sperm-specific histone 2B (TSH2B) has been detected in only ~30% of mature spermatozoa. Its level in the semen of subfertile patients varies; its function is unknown. We evaluated TSH2B in the sperm samples of 23 donors and 49 subfertile patients and assessed its association with chromatin compaction status. METHODS: TSH2B level was measured using immunoblotting. Chromatin packaging quality was evaluated by staining with chromomycin A3 (CMA3) which marked spermatozoa with defective packaging. To assess both TSH2B and chromatin status in the same spermatozoon, CMA3 staining and TSH2B immunolocalization were performed sequentially. RESULTS: A significant correlation (r = 0.55, P = 0.0027) was found between TSH2B level and percentage of CMA3-positive sperm in patient and donor semen samples. When individual spermatozoa were assessed for these parameters, 92% of TSH2B-containing cells were also CMA3 positive. Variation in the total sperm TSH2B level was less in donors than in patients. CONCLUSIONS: CMA3 positive staining of TSH2B-containing individual spermatozoa and a significant correlation between the total TSH2B level and CMA3 percentage in semen samples suggest a structural role for TSH2B in sperm chromatin organization. Low variability of TSH2B level in donors implies a mechanism (however unknown) regulating this parameter.

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Section: Discussionmentioning

confidence: 99%

Testis/sperm-specific histone 2B in the sperm of donors and subfertile patients: variability and relation to chromatin packaging

et al. 2006

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“…Similar changes with respect to increased ALH and reduction in progressive motility have been observed during hyperactivation of spermatozoa from guinea pigs (Katz et al, 1978;Katz and Yanagimachi, 19811, monkeys (Behboodi et al, 19871, rabbits (Johnson et al, 1981;Suarez et al, 19831, and rams Cummins, 1982). Increased ALH in hyperactivated spermatozoa is a significant functional change and has been correlated with the fertilizing ability of human spermatozoa (Aitken et al, 1985;Jeulin et al, 1986;Mortimer et al, 1986).…”

Section: The Results Inmentioning

confidence: 99%

Analysis of the motility parameters of in vitro hyperactivated hamster spermatozoa

Shivaji

Peedicayil

Devi

1995

Molecular Reproduction Devel

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Golden hamster cauda epididymal spermatozoa under in vitro capacitating conditions exhibited time-dependent transformation of motility pattern, and the frequency of occurrence of a particular motility type was found to be dependent on the depth of the motility chamber used. The nonhyperactivated spermatozoa with planar motility were the most predominant at 0 hr irrespective of the depth of the motility chamber. But spermatozoa with the helical motility pattern were not detectable up to 6 hr when the Makler chamber was used, whereas in both the slide chamber and cannula, by 2 hr such spermatozoa constituted 90% of the total spermatozoa. However, by 6 hr the hyperactivated circular moving spermatozoa were the predominant type in all the chambers. Sperm motility chamber depths were also found to effect the motility parameter values of hamster spermatozoa, but this effect was also found to be dependent on the type of motility. Increase in chamber depth did not alter any of the motility parameter values of spermatozoa with hatchet type of motility and only increased the amplitude of lateral head displacement (ALH) in planar type. But in spermatozoa with the helical type of motility, an increase in chamber depth increased the progressive velocity (VSL), path velocity (VAP), curvilinear velocity (VCL), straightness (STR), linearity (LIN), and ALH. In spermatozoa with the circular type of motility, an increase in VSL, VAP, VCL, and ALH was also observed, but STR and LIN decreased. The hyperactivated spermatozoa could be distinguished from the nonhyperactivated spermatozoa because the former were swimming in circles, had low progressive velocity, decreased straightness and linearity of path, and also exhibited an increase in the amplitude of lateral head displacement compared to the nonhyperactivated spermatozoa. Further, the spermatozoa with helical motility could be differentiated from the nonhyperactivated, circular, and hatchet spermatozoa in that they had the highest VSL, VAP, VCL, and ALH. Spermatozoa with hatchet movement were slow and exhibited very low STR and LIN. Thus the motility parameters could be used to distinguish the nonhyperactivated and hyperactivated distal cauda epididymal spermatozoa.

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“…Sur le plan morphologique, les anomalies de l'acrosome peuvent ~tre responsables d'6checs de f6condation [58]. Le cas le plus extr6me est la st6rilit6 d6finitive des sujets porteurs de spermatozoides ~ t~te ronde sans acrosome [59].…”

Section: Relation Entre La Ra Et La F6condan-ce Du Spermatozoideunclassified

La réaction acrosomique

Pilikian

1994

Androl.

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RESUME La c a p a c i t~ d e s s p e r m a t o z o i d e s a c c o m p l i r la r~a c t i o n a c r o s o m i I N T R O D U C T I O NLa fonction principale du spermatozo~de est d'apporter son ADN ~ l'ovocyte Pour cela il se sert ~ la fois de sa mobilit5 et de ses enzymes localis~es dans la partie la plus ant~rieure de sa t~te : L'ACROSOME.L'acrosome est une v~sicule aplatie entour~e d'une m e m b r a n e off l'on distingue la m e m b r a n e acrosomique interne face ~ la membrane nucl~aire et la membrane acrosomique externe face ~ la membrane plasmique du spermatozoade. Les membranes acrosomiques interne et externe se joignent au niveau du segment ~quatoriaL. L'acrosome peut ~tre assimil~ ~ une v~sicule s~cr~-toire ; les enzymes h y d r o l y t i q u e s qu'elle contient (acrosine, hyaluronidase, neuraminidase pour ne citer que les principales) sont lib6r6es par un ph~nom~ne d'exocytose suite ~ un signal appropri~ et participent v r a i s e m b l a b l e m e n t h la p r o g r e s s i o n des gametes males ~ travers les multiples enveloppes ovocytaires. L'activation ct la liberation s~lectives de ces enzymes ~ mesure que les spermatozoades approchent l'ovocyte, sont contr61~es par les ph~nom~nes complexes de capacitation et de r~action acrosomique, difficilement dissociables.La n~cessit~ pour les spermatozoa'des ~jacu-l~s de subir des modifications pour acqu~rir la capacit~ de f~conder les ovocytes a ~t~ d~crite pour la premiere fois en 1951 [1,2]. Depuis, malgr~ le nombre considerable de t r a v a u x il s u b s i s t e encore u n e c e r t a i n e confusion sur le sujet. Cela tient d'une part, aux conditions dans lesquelles ces ph~no-37

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Sperm factors related to failure of human in-vitro fertilization

Cited by 202 publications

References 17 publications

Testis/sperm-specific histone 2B in the sperm of donors and subfertile patients: variability and relation to chromatin packaging

Testis/sperm-specific histone 2B in the sperm of donors and subfertile patients: variability and relation to chromatin packaging

Analysis of the motility parameters of in vitro hyperactivated hamster spermatozoa

La réaction acrosomique

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