Sperm head defects and disturbances in spermatozoal chromatin and DNA integrities in idiopathic infertile subjects: Association with cigarette smoking

Elshal, Mohamed F.; Elsayed, Ibrahim; Elsaied, Moustafa A.; El‐Masry, Samir; Kumosani, Taha

doi:10.1016/j.clinbiochem.2008.11.012

Cited by 94 publications

(75 citation statements)

References 38 publications

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“…These fi ndings were very ing seminal cotinine as a measure of tobacco consumption. The present fi ndings agree with those of previous studies (1,3,(25)(26)(27)(28). On the contrary, some researches reported different results (29)(30)(31)(32).…”

Section: Discussionsupporting

confidence: 82%

“…Oxidants in cigarette smoke are thought to damage sperm DNA, and smokers have more oxidative DNA damage in their sperm than do non-smokers (39). An adverse effect of reactive oxygen species (ROS) on sperm quality is a possibility that could justify the observed sperm damage, since elevated levels of ROS have been found in infertile smoking men (28,40). Another causative factor would be the fact that seminal plasma in smokers contains lower levels of antioxidants than that of non smokers (28,41).…”

Section: Discussionmentioning

confidence: 99%

“…An adverse effect of reactive oxygen species (ROS) on sperm quality is a possibility that could justify the observed sperm damage, since elevated levels of ROS have been found in infertile smoking men (28,40). Another causative factor would be the fact that seminal plasma in smokers contains lower levels of antioxidants than that of non smokers (28,41). Sperm nuclear chromatin abnormalities/ DNA damage could occur at the time of or result from DNA packing at spermatogenesis (42).…”

Section: Discussionmentioning

confidence: 99%

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Apoptotic markers in semen of infertile men: association with cigarette smoking

El-Melegy

Ali

2011

Int. braz j urol.

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Objectives: (i) To examine the role of apoptosis in the pathogenesis of DNA damage in semen from infertile men. (ii) To assess the effects of smoking on apoptotic markers and seminal parameters among infertile men. (iii) To assess the correlation of apoptosis with conventional semen parameters. Materials and Methods:The study was carried out on 70 men with idiopathic infertility, divided into two groups: thirty infertile non smokers and forty infertile smokers. In addition to 60 fertile men (30 non smokers and 30 smokers) as control group. Each subject provided semen for analysis of parameters, determination of % of DNA fragmentation, s-Fas, caspase-3 activity levels and cotinine levels. Results:The results revealed that infertile men, particularly smokers have significantly lower semen variables and significantly higher levels of apoptotic variables (% of DNA fragmentation, s-Fas and caspase-3 activity) in addition to cotinine. Conclusions:The present findings provide additional evidence supporting the importance of the evaluation of apoptotic markers to test male infertility particularly among smokers.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Apoptotic markers in semen of infertile men: association with cigarette smoking

El-Melegy

Ali

2011

Int. braz j urol.

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“…It is reported that smokers had a 48% increase in seminal leukocyte levels and a 107% increase in seminal ROS levels compared with nonsmokers [39]. The sperm DNA fragmentation index (DFI) is also increased in infertile smokers compared with infertile nonsmokers (37.66% vs. 14.51%, P < 0.001) [40]. Furthermore, natural antioxidants such as vitamins C and E in seminal plasma were decreased in smokers, which indicates a reduced protection against oxidative stress [41].…”

Section: External Causes Of Potential Oxidative Stressmentioning

confidence: 76%

Reactive Oxygen Species and Sperm Cells

Takeshima¹,

Kuroda²,

Yumura³

2018

Reactive Oxygen Species (ROS) in Living Cells

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Many cases of male factor infertility are idiopathic, but 30-40% of cases may have excessive levels of reactive oxygen species (ROS) in their semen. The origins of endogenous ROS are leukocytes and immature spermatozoa, and external causes are various. On the contrary, seminal plasma contains various antioxidants. Low levels of ROS are essential for the fertilization process, but excessive levels of ROS lead to oxidative stress and can have harmful effects such as lipid peroxidation of a membrane, sperm deoxyribonucleic acid fragmentation, and apoptosis on the fertile capacity. In order to evaluate oxidative stress appropriately, ROS is measured by the chemiluminescence method with neat semen and quantification of 8-OH-2′-deoxyguanosine and malondialdehyde in seminal plasma. Antioxidant potential is often measured using total antioxidant capacity (TAC) assay. The oxidation-reduction potential measured by a MiOXSYS analyzer is a novel, easier, quicker, and less expensive technology to measure oxidative stress. In order to minimize oxidative stress and improve clinical outcomes, sperm-sorting methods, lifestyle modifications, shortening the ejaculatory abstinence, and treatments such as oral antioxidants, varicocelectomy, and testicular sperm extraction are taken into account. As a future prospect, proteomics, metabolomics, and genomics are still developing areas that have the potential to discover new findings and highly sensitive biomarkers.

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“…Paternal smoking has been reported to introduce sperm DNA damage [60] and an increased risk of childhood cancer in the offspring in some studies [61][62][63]. However, others could not find these associations [64,65].…”

Section: The Impact Of Environment and Lifestyle On The Genome Of Spementioning

confidence: 67%

Gene-environment interaction and male reproductive function

Axelsson¹,

Bonde²,

Giwercman³

et al. 2010

Asian J Androl

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As genetic factors can hardly explain the changes taking place during short time spans, environmental and lifestyle-related factors have been suggested as the causes of time-related deterioration of male reproductive function. However, considering the strong heterogeneity of male fecundity between and within populations, genetic variants might be important determinants of the individual susceptibility to the adverse effects of environment or lifestyle. Although the possible mechanisms of such interplay in relation to the reproductive system are largely unknown, some recent studies have indicated that specific genotypes may confer a larger risk of male reproductive disorders following certain exposures. This paper presents a critical review of animal and human evidence on how genes may modify environmental effects on male reproductive function. Some examples have been found that support this mechanism, but the number of studies is still limited. This type of interaction studies may improve our understanding of normal physiology and help us to identify the risk factors to male reproductive malfunction. We also shortly discuss other aspects of gene-environment interaction specifically associated with the issue of reproduction, namely environmental and lifestyle factors as the cause of sperm DNA damage. It remains to be investigated to what extent such genetic changes, by natural conception or through the use of assisted reproductive techniques, are transmitted to the next generation, thereby causing increased morbidity in the offspring.

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Sperm head defects and disturbances in spermatozoal chromatin and DNA integrities in idiopathic infertile subjects: Association with cigarette smoking

Cited by 94 publications

References 38 publications

Apoptotic markers in semen of infertile men: association with cigarette smoking

Apoptotic markers in semen of infertile men: association with cigarette smoking

Reactive Oxygen Species and Sperm Cells

Gene-environment interaction and male reproductive function

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