Sperm kinematics and subpopulational responses during the cryopreservation process in caprine ejaculates

Barbas, João Pedro; Leahy, Tamara; Horta, A.E.M.; García-Herreros, Manuel

doi:10.1016/j.cryobiol.2018.03.005

Cited by 16 publications

(12 citation statements)

References 46 publications

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“…[95] On the other hand, it is widely known that different sperm subpopulations with distinct kinematic, functional and morphometric characteristics coexist in the ejaculates [105][106][107], which leads to interand intra-male variations. The different steps of freezing-thawing process have been shown to alter the distribution of these kinematic and morphometric sperm subpopulations originally present in fresh ejaculates of ram, bull and buck [108][109][110]. These findings suggest that those fresh ejaculates with a higher subpopulation of sperm exhibiting a fast and progressive motility pattern as well as a low sperm head area could resist more the cryopreservation process.…”

Section: Protein Namementioning

confidence: 94%

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Peris-Frau¹,

Soler²,

Iniesta‐Cuerda³

et al. 2020

IJMS

149

118

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Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm cryopreservation in different ruminant species. However, a significant amount of sperm still suffers considerable cryodamage, which may affect sperm quality and fertility. Recently, the use of different “omics” technologies in sperm cryobiology, especially proteomics studies, has led to a better understanding of the molecular modifications induced by sperm cryopreservation, facilitating the identification of different freezability biomarkers and certain proteins that can be added before cryopreservation to enhance sperm cryosurvival. This review provides an updated overview of the molecular mechanisms involved in sperm cryodamage, which are in part responsible for the structural, functional and fertility changes observed in frozen–thawed ruminant sperm. Moreover, the molecular basis of those factors that can affect the sperm freezing resilience of different ruminant species is also discussed as well as the molecular aspects of those novel strategies that have been developed to reduce sperm cryodamage, including new cryoprotectants, antioxidants, proteins, nanoparticles and vitrification.

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Section: Protein Namementioning

confidence: 94%

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Peris-Frau¹,

Soler²,

Iniesta‐Cuerda³

et al. 2020

IJMS

149

118

View full text Add to dashboard Cite

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“…The computer-assisted sperm analysis (CASA) allows assessing the sperm kinetics and the individual record of their trajectories (Amann & Waberski, 2014;Barbas et al, 2018;Holt & Satake, 2018), based on multivariate statistical analyses to identify and monitor the behaviour of sperm subpopulations within an ejaculate (Souza et al, 2018). The determination of sperm subpopulations based on individual sperm kinetics has been reported in animal species such as domestic cats (Gutiérrez-Reinoso & García-Herreros, 2016;Souza et al, 2018), dogs (Núñez-Martínez et al, 2006;Peña et al, 2012), stallions (Quintero-Moreno et al, 2003), donkeys (Flores et al, 2008), sheep (Barbas et al, 2018;Bergstein-Galan et al, 2017;Dorado et al, 2011;Santolaria et al, 2015), boars (Estrada et al, 2017;Flores et al, 2009), rabbits (Quintero-Moreno et al, 2007) and red deer (Martinez-Pastor et al, 2005). In bulls, studies that evaluated fresh semen and post-thawed samples identified four sperm subpopulations (Ferraz et al, 2014;Ibanescu et al, 2020;Muiño et al, 2009;Muiño, Rivera, et al, 2008;Muiño, Tamargo, et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the study of the sperm subpopulations in an ejaculate, provides relevant information regarding the functional diversity of the sperm groups (Yániz et al, 2018), ensuring a more accurate analysis. Because, the average values can mask the effects of various treatments on spermatozoa and not consider the intrinsic variability in the ejaculate (Barbas et al, 2018;Holt & Satake, 2018), causing loss of important information (Martinez-Pastor et al, 2011). In addition, the characterization of the sperm subpopulations allows to evidence differences between the experimental groups, between different treatments, in which many of the effects are still unknown (Amann & Waberski, 2014;Kathiravan et al, 2011) and also be used to predict herd reproductive performance (Kathiravan et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Sperm subpopulations influence the pregnancy rates in cattle

Hidalgo

Almeida

Moraes³

et al. 2021

Reprod Domestic Animals

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This study aimed to study the characteristics and subpopulations of spermatozoa from bulls with low and high reproductive performance based on pregnancy rates.Based on historical records of pregnancy rate from four farms, 24 bulls were selected. Two groups were established, with low pregnancy rates (n = 12; LOW), including bulls that presented pregnancy rates <52.27% (33.33% to 51.81%); and a group with high pregnancy rates (n = 12; HIGH), with pregnancy rates >52.27% (52.27% to 69.64%), after fixed-time artificial insemination (FTAI). The thawed sperm straws were analysed to sperm kinetics, morphology, plasma membrane integrity and sperm subpopulations. The LOW group exhibited a higher proportion of static cells (p < .05).In contrast, the HIGH group showed greater percentages for membrane integrity and total and progressive motility, and cells with fast and medium velocity (p < .05). In the cluster procedures, four sperm subpopulations were established. The low-fertility bulls presented the highest percentage of subpopulation 2 (41.46%), characterized by slow and progressive spermatozoa. The high-fertility bulls exhibited the highest percentage of subpopulation 3 (37.17%), characterized by fast and nonlinear spermatozoa. Results from this study indicated that bulls with greater percentages of fast and nonlinear spermatozoa seem to have greater fertilization capacity and the subpopulations analysis can be considered a tool to identify ejaculates with high fertility.

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“…The motility pattern of each subpopulation and the proportion of spermatozoa distributed within them can also be affected by the cryopreservation process. In several species, freezing—thawing procedures altered those motile sperm subpopulations present in fresh ejaculates [ 4 , 22 , 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of Cryopreservation on Motile Subpopulations and Tyrosine-Phosphorylated Regions of Ram Spermatozoa during Capacitating Conditions

Peris-Frau¹,

Sánchez-Ajofrín²,

Maestro³

et al. 2021

Biology

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The heterogeneous nature of ejaculates highlights the relevance of studying the behavior of different sperm subpopulations. Changes in sperm motility and the increase in tyrosine phosphorylation are key events that usually occur during capacitation and can be modified by the cryopreservation process. However, the relationship between both events remains poorly defined throughout capacitation in the different sperm subpopulations. Fresh and frozen-thawed spermatozoa were incubated in capacitating (CAP) and non-capacitating (NC) media up to 240 min. Sperm kinematics, tyrosine phosphorylation and mitochondrial activity were measured by the CASA system and imaging flow cytometry. Four motile sperm subpopulations (SP) were identified in fresh and frozen-thawed ram semen after the cluster analysis. Incubation under CAP conditions over time led to greater changes in the percentage of spermatozoa included in each subpopulation compared to NC conditions, being different between fresh and frozen-thawed spermatozoa. The SP1, characterized by slow spermatozoa, progressively increased after 15 min in frozen-thawed samples incubated in both media but not in fresh ones. The SP4, characterized by fast and non-linear spermatozoa, showed a marked increase during CAP, but not under NC conditions, occurring more rapidly in frozen-thawed spermatozoa. This subpopulation (SP4) was also the only one positively and strongly correlated with mitochondrial activity and all phosphorylated sperm regions during capacitation, either in fresh or frozen-thawed samples. Our results indicated that in vitro capacitation induced significant changes in the distribution of motile sperm subpopulations, being affected by cryopreservation. Notwithstanding, the subpopulation which probably represents hyperactivated-like spermatozoa (SP4) also increased in frozen-thawed samples, occurring faster and simultaneously to the increment of mitochondrial activity and tyrosine phosphorylation of different sperm regions.

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Sperm kinematics and subpopulational responses during the cryopreservation process in caprine ejaculates

Cited by 16 publications

References 46 publications

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Sperm subpopulations influence the pregnancy rates in cattle

Impact of Cryopreservation on Motile Subpopulations and Tyrosine-Phosphorylated Regions of Ram Spermatozoa during Capacitating Conditions

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