Sperm maturation and capacitation in the open thelycum shrimp Litopenaeus (Crustacea: Decapoda: Penaeoidea)

Alfaro, Jorge; Ulate, Karol; Vargas, Maribelle

doi:10.1016/j.aquaculture.2007.05.011

Cited by 48 publications

(89 citation statements)

References 15 publications

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“…In other open-thelycal crustacean species, there is evidence that sperm maturation and capacitation are completed while they are still inside the SD (Alfaro et al 2007). This is different to crustacean species that have closed thelyca, where sperm maturation and capacitation are completed in the female after insemination (Vanichviriyakit et al 2004) and is more like vertebrates, where sperm are known to require storage and receiving essential stimulatory molecules from the female reproductive tract (Austin 1952;Chang 1951).…”

Section: Discussionmentioning

confidence: 95%

“…However, in penaeid shrimps having open thelyca, it has been postulated that the process of sperm maturation and capacitation had to be completed in the male reproductive tract (Clark and Griffin 1993), as sperm of these shrimps are attached to the open thelycal space within the female abdomen following copulation. Furthermore, it was reported that in Litopenaeus vannamei shrimp, which has an open thelycum, the spermatophores within the terminal ampoules contain fully mature and capacitated sperm (Alfaro et al 2007). Recent studies in two penaeid shrimps, P. monodon (Vanichviriyakit et al 2004) and L. vannamei (Aungsuchawan et al 2011) indicated that, similarly to mammalian sperm, tyrosine phosphorylated proteins are present in thelycal fluid and may be involved in capacitating sperm.…”

Section: Introductionmentioning

confidence: 97%

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Expression of the male reproduction-related gene (Mar-Mrr) in the spermatic duct of the giant freshwater prawn, Macrobrachium rosenbergii

et al. 2012

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Phosphorylated sperm proteins are crucial for sperm maturation and capacitation as a priori to their fertilization with eggs. In the freshwater prawn, Macrobrachium rosenbergii, a male reproduction-related protein (Mar-Mrr) was known to be expressed only in the spermatic ducts as a protein with putative phosphorylation and may be involved in sperm capacitation in this species. We investigated further the temporal and spatial expression of the Mar-Mrr gene using RT-PCR and in situ hybridization and the characteristics and fate of the protein using immunblotting and immunocytochemistry. The Mar-Mrr gene was first expressed in 4-week-old post larvae and the protein was produced in epithelial cells lining the spermatic ducts, at the highest level in the proximal region and decreased in the middle and distal parts. The native protein had a MW of 17 kDa and a high degree of serine/threonine phosphorylation. It was transferred from the epithelial cells to become a major protein at the anterior region of the sperm. We suggest that it is involved in sperm capacitation and fertilization in this open thelycal species and this is being investigated.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 97%

Expression of the male reproduction-related gene (Mar-Mrr) in the spermatic duct of the giant freshwater prawn, Macrobrachium rosenbergii

et al. 2012

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“…The result also remarkably demonstrates that before AR E. sinensis spermatozoa possess the physiological process relating to AR. It might be the process of capacitation, which has been found in the shrimp Sicyonia ingentis (Crustacea: Decapoda) (Lindsay and Clark 1992) and Litopenaeus (Crustacea: Decapoda) (Alfaro et al 2007) but has not been known in E. sinensis. But our result, that the spermatozoa treated by low temperature but not by 15 and 4°C can undergo AR, indicates that the spermatozoa can be affected physiologically to capacitate with low temperature, which is coincident with the results published by Du and Xue (1987).…”

Section: Discussionmentioning

confidence: 99%

Induction of acrosome reaction of spermatozoa in Eriocheir sinensis by low temperature

Kang

2010

Cytotechnology

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The effects of temperatures, durations of treatment, and derivations from spermatophores or spermaries on in vitro acrosome reaction of the spermatozoa in the Chinese mitten crab Eriocheir sinensis were investigated. The results showed that the different temperatures resulted in extremely significant differences (p \ 0.01) in the time of beginning acrosome reaction, the time of the maximum percentage of acrosome reaction, and the maximum percentage of acrosome reaction of the spermatozoa from spermatophores; and the low temperature (-20, -80°C and liquid nitrogen) induced acrosome reaction of more than 90% spermatozoa while 15 and 4°C didn't. Similar results occur in the spermatozoa, treated with -80°C for 15 min, from spermaries but the time of beginning acrosome reaction and the time of the maximum percentage of acrosome reaction were obviously longer than those from spermatophores. In conclusion, low temperature can induce acrosome reaction, which is a novel and efficient operating method of inducing acrosome reaction; the spermatozoa might be affected physiologically to capacitate with chilling. The study may be beneficial to new understandings of mechanism of acrosome reaction and provide the foundational material for artificial fertilization and breeding of this crab and other commercial aquatic crustaceans.

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“…Los autores concluyeron que la maduración acrosómica depende de la síntesis total de la malla filamentosa. Además, determinaron que después del apareamiento, las células espermáticas maduras de L. occidentalis sufren una serie de cambios fisiológicos que le otorgan la capacidad de reaccionar con los ovocitos presentes en el agua (Alfaro, Ulate & Vargas, 2007). Seguidamente, se evaluó el efecto de la hormona juvenil III y del farnesoato de metilo sobre la calidad de espermatóforos en L. vannamei.…”

Section: Líneas De Investigación Desarrolladas § Moluscosunclassified

Avances biotecnológicos sobre maricultura en Costa Rica. Una revisión de la investigación desarrollada por la Escuela de Ciencias Biológicas de la Universidad Nacional

Rojas-Alfaro¹,

Sancho-Blanco²,

Vega-Corrales³

2017

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El desarrollo de la maricultura en Costa Rica ha requerido de la validación, la innovación y la generación de conocimiento científico biotecnológico. Se presenta una revisión de los principales avances biotecnológicos sobre maricultura que han sido aplicados y desarrollados por la Escuela de Ciencias Biológicas de la Universidad Nacional de Costa Rica. La experimentación y optimización de la tecnología del cultivo de Crassostrea gigas ha impulsado la ostricultura en el país y el surgimiento de emprendimientos locales. La investigación científica realizada ha generado avances biotecnológicos de relevancia mundial sobre la biología reproductiva de camarones peneidos y ha permitido la reproducción controlada de peces marinos de interés comercial del Golfo de Nicoya, Costa Rica. Además, se han aplicado y evaluado técnicas biotecnológicas para el cultivo de microalgas, depuración bacteriana de moluscos, vigilancia de Floraciones Algales Nocivas y biorremediación. Se debe fortalecer la investigación biotecnológica sobre maricultura, fomentar la transferencia de la biotecnología desarrollada y promover la protección por medio de la propiedad intelectual de la innovación biotecnológica generada como herramientas para el desarrollo económico y social en Costa Rica.

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Sperm maturation and capacitation in the open thelycum shrimp Litopenaeus (Crustacea: Decapoda: Penaeoidea)

Cited by 48 publications

References 15 publications

Expression of the male reproduction-related gene (Mar-Mrr) in the spermatic duct of the giant freshwater prawn, Macrobrachium rosenbergii

Expression of the male reproduction-related gene (Mar-Mrr) in the spermatic duct of the giant freshwater prawn, Macrobrachium rosenbergii

Induction of acrosome reaction of spermatozoa in Eriocheir sinensis by low temperature

Avances biotecnológicos sobre maricultura en Costa Rica. Una revisión de la investigación desarrollada por la Escuela de Ciencias Biológicas de la Universidad Nacional

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