Sperm migration, selection, survival, and fertilizing ability in the mammalian oviduct

Mahé, Coline; Zlotkowska, Aleksandra Maria; Reynaud, Karine; Tsikis, Guillaume; Mermillod, Pascal; Druart, Xavier; Schoen, Jennifer; Saint‐Dizier, Marie

doi:10.1093/biolre/ioab105

Cited by 43 publications

(37 citation statements)

References 151 publications

(305 reference statements)

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“…Finally, round spermatids are transformed into spermatozoa through complex morphological changes, including chromatin remodeling and compaction ( Guercio et al, 2020 ). The formed spermatozoa are transported to the epididymis for maturation and storage, where they gaining motility and acrosomal function for subsequent capacitation and fertilization in the female reproductive tract ( Mahe et al, 2021 ). Spermatogenesis is regulated by hormones, local regulators, and miRNAs, which ensures the correct genetic and epigenetic information, is transmitted to the offspring.…”

Section: Introductionmentioning

confidence: 99%

Taste receptors affect male reproduction by influencing steroid synthesis

Liu

Gong

Shi

et al. 2022

Front. Cell Dev. Biol.

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For the male genetic materials to reach and fertilize the egg, spermatozoa must contend with numerous environmental changes in a complex and highly sophisticated process from generation in the testis, and maturation in the epididymis to capacitation and fertilization. Taste is an ancient chemical sense that has an essential role in the animal’s response to carbohydrates in the external environment and is involved in the body’s energy perception. In recent years, numerous studies have confirmed that taste signaling factors (taste receptor families 1, 2 and their downstream molecules, Gα and PLCβ2) are distributed in testes and epididymis tissues outside the oral cavity. Their functions are directly linked to spermatogenesis, maturation, and fertilization, which are potential targets for regulating male reproduction. However, the specific signaling mechanisms of the taste receptors during these processes remain unknown. Herein, we review published literature and experimental results from our group to establish the underlying signaling mechanism in which the taste receptor factors influence testosterone synthesis in the male reproduction.

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Section: Introductionmentioning

confidence: 99%

Taste receptors affect male reproduction by influencing steroid synthesis

Liu

Gong

Shi

et al. 2022

Front. Cell Dev. Biol.

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“…Previous reports indicate that sperm binding with the oviduct could induce a subcellular reorganization of certain sperm proteins [ 6 , 8 , 10 , 28 ]. Taking this into account, we evaluated the localization of α5β1 in sperm released from heterologous co-cultures.…”

Section: Resultsmentioning

confidence: 99%

“…Once ejaculated, sperm reach the female reproductive tract which provides the cellular and molecular microenvironment needed to acquire fertilizing ability [5,6]. The interaction between sperm receptors and molecules such as proteins, glycosaminoglycans, or lipids from the oviductal fluid, is essential for the success of mammalian fertilization [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of sperm integrin α5β1 as a potential marker of fertility in humans

et al. 2022

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Sperm selection for assisted reproduction techniques is generally based on basic parameters, while key aspects of sperm competence and its journey from the deposition site to the fertilization site are overlooked. Consequently, identifying molecular markers in spermatozoa that can efficiently predict the fertility of a semen sample could be of great interest, particularly in cases of idiopathic male infertility. When spermatozoa reach the female reproductive tract, it provides to them the cellular and molecular microenvironment needed to acquire fertilizing ability. In this sense, considering the role that integrin α5β1 of spermatozoa plays in reproduction-related events, we investigated the correlation between the subcellular localization of sperm integrin α5β1 and early embryo development outcome after in vitro fertilization (IVF) procedures in human. Twenty-four semen samples from normozoospermic men and metaphase II (MII) oocytes from healthy women aged under 38 years, from couples who underwent IVF cycles, were used in this work. Sperm α5β1 localization was evaluated by immunofluorescence assay using an antibody against integrin α5 subunit. Integrin α5β1 was mainly localized in the sperm acrosomal region (45.33±7.89%) or the equatorial segment (30.12±7.43%). The early embryo development rate (data obtained from the Fertility Center) correlated positively with the localization of α5β1 in the acrosomal region (number of usable embryos / inseminated oocytes: ρ = 0.75; p<0.01 and number of usable embryos/total number of two pronuclear zygotes: ρ = 0.80; p<0.01). However, this correlation was not significant when the equatorial segment mark was evaluated. In addition, human sperm released from co-culture with bovine oviductal epithelial cells (BOEC) showed a significant enrichment in the acrosomal localization pattern of α5β1 compared to those sperm that were not co-cultured with BOEC (85.20±5.35% vs 35.00±17.09%, respectively, p<0.05). In conclusion, the evaluation of sperm integrin α5β1 immunolocalization could be a useful tool to select sperm with fertilizing ability from human semen samples before IVF procedures.

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“…Sperm cells bind preferentially to ciliated epithelial cells [56] through specific carbohydrate residues or the lectin-like proteins, thus forming the sperm reservoir [53,57]. Only motile, non-capacitated, acrosome-intact and normal chromatin structure spermatozoa are able to bind to the oviductal epithelium [58], extending their lifespan and delaying capacitation [59].…”

Section: Sperm Capacitation On Soecs Previously Supplemented With P4 ...mentioning

confidence: 99%

Pre-Treatment of Swine Oviductal Epithelial Cells with Progesterone Increases the Sperm Fertilizing Ability in an IVF Model

Cimini

Moussa

Taraschi

et al. 2022

Animals

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Mammalian spermatozoa are infertile immediately after ejaculation and need to undergo a functional modification, called capacitation, in order to acquire their fertilizing ability. Since oviductal epithelial cells (SOECs) and progesterone (P4) are two major modulators of capacitation, here we investigated their impact on sperm functionality by using an IVF swine model. To that, we treated SOECs with P4 at 10, 100, and 1000 ng/mL before the coincubation with spermatozoa, thus finding that P4 at 100 ng/mL does not interfere with the cytoskeleton dynamics nor the cells’ doubling time, but it promotes the sperm capacitation by increasing the number of spermatozoa per polyspermic oocyte (p < 0.05). Moreover, we found that SOECs pre-treatment with P4 100 ng/mL is able to promote an increase in the sperm fertilizing ability, without needing the hormone addition at the time of fertilization. Our results are probably due to the downregulation in the expression of OVGP1, SPP1 and DMBT1 genes, confirming an increase in the dynamism of our system compared to the classic IVF protocols. The results obtained are intended to contribute to the development of more physiological and efficient IVF systems.

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Sperm migration, selection, survival, and fertilizing ability in the mammalian oviduct

Cited by 43 publications

References 151 publications

Taste receptors affect male reproduction by influencing steroid synthesis

Taste receptors affect male reproduction by influencing steroid synthesis

Evaluation of sperm integrin α5β1 as a potential marker of fertility in humans

Pre-Treatment of Swine Oviductal Epithelial Cells with Progesterone Increases the Sperm Fertilizing Ability in an IVF Model

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