Sperm Mitochondrial DNA Elimination in the Zygote of the Oogamous Brown Alga Undaria pinnatifida (Laminariales, Phaeophyceae)

Kimura, Kei; Nagasato, Chikako; Uwai, Shinya; Motomura, Taizo

doi:10.1508/cytologia.75.353

Cited by 7 publications

(3 citation statements)

References 35 publications

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“…Our observations are in line with detection of the anchor flagella in Saccharina (Motomora 1991; Klochkova et al, 2019). The cell division pattern of Undaria embryos, with cells initially developing a unilayered 2-dimensional blade, are similar as described by Kimura et al (2010), Klochkova et al (2019) and Theodorou (2023). Like in Arabidopsis (Bayer et al, 2017) and Dictyota (Bogaert et al, 2017a, 2017b) the spherical egg cell undergoes an elongation along the apical-basal axis.…”

Section: Discussionsupporting

confidence: 72%

Cell wall-mediated maternal control of apical-basal patterning of the kelpUndaria pinnatifida

Dries,

Meyers,

Liesner

et al. 2024

Preprint

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The role of maternal tissue in the control of embryogenesis remains enigmatic in many complex organisms. Here, we investigate the contribution of maternal tissue to apical-basal patterning in the kelp embryo. Using a modified kelp fertilisation protocol which yields synchronously developing kelp embryos, we show that detachment from maternal tissue leads to compromised robustness of apical-basal patterning. Detached embryos are rounder and often show aberrant morphologies. Furthermore, absence of contact with maternal tissue increases parthenogenesis, highlighting the critical role of maternal signals in the initial stages of kelp development. When zygotes are detached from the female gametophyte while part of the oogonial cell wall still remains attached to the egg, the proper apical-basal patterning is rescued showing a key role for the connection to the maternal cell wall in developmental patterning in kelps. This observation is reminiscent of another brown alga, Fucus, where the cell wall has been shown to play a key role in cell fate determination. In the case of kelps, the maternal oogonium mediates basal cell fate determination by providing an extrinsic patterning cue in its extracellular matrix to the future embryo. Our findings suggest a conserved mechanism across phylogenetically distant oogamous brown algal lineages, where localised secretion of sulphated F2 fucans mediate establishment of the apical-basal polarity.

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Section: Discussionsupporting

confidence: 72%

Cell wall-mediated maternal control of apical-basal patterning of the kelpUndaria pinnatifida

Dries,

Meyers,

Liesner

et al. 2024

Preprint

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show abstract

“…203, the artificial hybrid strain between the female gametophyte of U. pinnatifida and the male gametophyte of U. peterseniana, shows similar morphological features with U. crenata. These results of molecular and morphological features strongly support the hybridization between two species, because only female gametophyte inherits mitochondria to the sporophyte of oogamous brown algae (Kimura et al 2010.…”

Section: Population Structure Of Saccharina Japonica In Koreasupporting

confidence: 66%

Kelps in Korea: from population structure to aquaculture to potential carbon sequestration

Hwang¹,

Boo²,

Graf³

et al. 2022

Algae

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Korea is one of the most advanced countries in kelp aquaculture. The brown algae, Undaria pinnatifida and Saccharina japonica are major aquaculture species and have been principally utilized for human food and abalone feed in Korea. This review discusses the diversity, population structure and genomics of kelps. In addition, we have introduced new cultivar development efforts considering climate change, and potential carbon sequestration of kelp aquaculture in Korea. U. pinnatifida showed high diversity within the natural populations but reduced genetic diversity in cultivars. However, very few studies of S. japonica have been conducted in terms of population structure. Since studies on cultivar development began in early 2000s, five U. pinnatifida and one S. japonica varieties have been registered to the International Union for the Protection of New Varieties of Plants (UPOV). To meet the demands for seaweed biomass in various industries, more cultivars should be developed with specific traits to meet application demands. Additionally, cultivation technologies should be diversified, such as integrated multi-trophic aquaculture (IMTA) and offshore aquaculture, to achieve environmental and economic sustainability. These kelps are anticipated to be important sources of blue carbon in Korea.

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“…The cell division pattern of Undaria embryos, with cells initially developing a unilayered 2‐dimensional blade, are similar as described by Kimura et al . (2010), Klochkova et al . (2019) and Theodorou & Charrier (2023).…”

Section: Discussionmentioning

confidence: 99%

Cell wall‐mediated maternal control of apical–basal patterning of the kelp Undaria pinnatifida

Dries,

Meyers,

Liesner

et al. 2024

New Phytologist

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Summary The role of maternal tissue in embryogenesis remains enigmatic in many complex organisms. Here, we investigate the contribution of maternal tissue to apical–basal patterning in the kelp embryo. Focussing on Undaria pinnatifida, we studied the effects of detachment from the maternal tissue using microsurgery, staining of cell wall modifications, morphometric measurements, flow cytometry, genotyping and a modified kelp fertilisation protocol synchronising kelp embryogenesis. Detached embryos are rounder and often show aberrant morphologies. When a part of the oogonial cell wall remains attached to the zygote, the apical–basal patterning is rescued. Furthermore, the absence of contact with maternal tissue increases parthenogenesis, highlighting the critical role of maternal signals in the initial stages of development. These results show a key role for the connection to the maternal oogonial cell wall in apical–basal patterning in kelps. This observation is reminiscent of another brown alga, Fucus, where the cell wall directs the cell fate. Our findings suggest a conserved mechanism across phylogenetically distant oogamous lineages, where localised secretion of sulphated F2 fucans mediates the establishment of the apical–basal polarity. In this model, the maternal oogonial cell wall mediates basal cell fate determination by providing an extrinsic patterning cue to the future kelp embryo.

show abstract

Sperm Mitochondrial DNA Elimination in the Zygote of the Oogamous Brown Alga Undaria pinnatifida (Laminariales, Phaeophyceae)

Cited by 7 publications

References 35 publications

Cell wall-mediated maternal control of apical-basal patterning of the kelpUndaria pinnatifida

Cell wall-mediated maternal control of apical-basal patterning of the kelpUndaria pinnatifida

Kelps in Korea: from population structure to aquaculture to potential carbon sequestration

Cell wall‐mediated maternal control of apical–basal patterning of the kelp Undaria pinnatifida

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