Sperm Mobility: Phenotype in Roosters (Gallus domesticus) Determinedby Concentration of Motile Sperm and Straight Line Velocity1

Froman, D. P.; Feltmann, A. J.

doi:10.1095/biolreprod62.2.303

Cited by 67 publications

(51 citation statements)

References 9 publications

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“…Computer-assisted sperm motion analysis was performed as outlined by Froman and Feltmann (2000) using 10 replicate roosters. Reagents were purchased from Sigma-Aldrich, St. Louis, MO.…”

Section: Methodsmentioning

confidence: 99%

Short-term preservation of fowl sperm in buffered potassium chloride

Froman

2013

Poultry Science

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Previous research demonstrated that sperm motility is dependent upon mitochondrial calcium cycling. Thus, sperm are inactivated when extracellular calcium ions are chelated. Mitochondrial calcium cycling, however, is driven by extracellular sodium ions. The hypothesis that sperm inactivation is subject to 2 variables was tested in the present work. Sperm motility was evaluated with computer-assisted sperm motion analysis in the first experiment. Sperm became immotile within minutes when suspended in buffered isotonic potassium chloride containing calcium ions. This outcome set the stage for the second experiment in which sperm were inactivated by centrifugation through 12% (wt/vol) Accudenz prepared with potassium chloride and tetrasodium 1,2-bis-(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA). Sperm mobility was the end point in the second and subsequent experiments. Potassium chloride was a suitable medium in regard to sperm inactivation with BAPTA followed by storage for 2 h at 10°C. Consequently, sperm so inactivated were reactivated after 1, 2, 3, 4, and 5 h of storage in the third experiment. Whereas pre-and postwash sperm mobility were equivalent, sperm mobility declined with time (P < 0.001) without exogenous energy in the storage medium. Therefore, the effect of 5 mM glucose was tested in the fourth experiment. In this case, recovery of sperm mobility was independent of time when sperm were stored at 10°C for 5 h (P > 0.05). Potassium chloride was replaced with potassium glutamate in the last experiment. Whereas reactivation was once again independent of time when sperm were stored with glucose (P > 0.05), greater variability was observed among observations in comparison with the potassium chloride-based medium. In summary, sperm motility was inactivated when calcium was chelated and extracellular sodium was replaced with potassium. Sperm reactivation was most consistent when chloride was the predominant extracellular anion. Collectively, these experiments demonstrate that short-term sperm storage can be achieved with simple media that promote sperm glycolysis and minimize energy demands imposed by the active transport of calcium and sodium ions.

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“…Computer-assisted sperm motion analysis was performed as outlined by Froman and Feltmann (2000) using 10 replicate roosters. Reagents were purchased from Sigma-Aldrich, St. Louis, MO.…”

Section: Methodsmentioning

confidence: 99%

Short-term preservation of fowl sperm in buffered potassium chloride

Froman

2013

Poultry Science

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“…We also recorded the number of sperm cells detected by the CASA plug-in because moving sperm are likely to interfere more with each other at higher densities, a parameter we could not control. Sperm velocity has previously been described using VSL, VAP, or VCL or a combination of these parameters (Holt et al 1997;Froman and Feltmann 2000;Gage et al 2004;Pizzari et al 2004). In our samples, these parameters were strongly correlated with each other (r, 0.93-0.998; all P<0.0001).…”

Section: Sperm Velocity and Sperm Longevitymentioning

confidence: 99%

Sperm morphology, swimming velocity, and longevity in the house sparrow Passer domesticus

Helfenstein

Podevin

Richner

2009

Behav Ecol Sociobiol

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Sperm competition exerts strong selection on males to produce spermatozoa with an optimal morphology that maximizes their fertilization success. Long sperm were first suggested to be favored because they should swim faster. However, studies that investigated the relationship between sperm length and sperm competitive ability or sperm swimming velocity yielded contradictory results. More recently, ratios of the different sections of a spermatozoon (the head, midpiece, and flagellum) were suggested to be more crucial in determining swimming velocity. Additionally, sperm ability to remain and survive in the female storage organs may also influence fertilization success, so that optimal sperm morphology may rather maximize sperm longevity than velocity. In this study, we investigated how sperm morphology is related to sperm velocity and sperm longevity in the house sparrow Passer domesticus. Sperm velocity was found to be correlated with head/flagellum ratio. Sperm with small heads relative to their flagellum showed higher swimming velocity. Additionally, shorter sperm were found to live longer. Finally, we found sperm morphological traits to vary substantially within males and the head/flagellum ratio to be unrelated to total sperm length. We discuss the hypothesis that the substantial within-male variation in sperm morphology reflects a male strategy to produce a diversity of sperm from long, fast-swimming to short, long-living sperm to maximize their fertilization success in a context of sperm competition.

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Section: Post Insemination Strategies Malesmentioning

confidence: 99%

“…Sperm quality measured as 'sperm mobility', an in vitro assay which measures the ability of sperm to penetrate a solution of an inert medium Froman and Feltmann, 2000). Sperm mobility is a normally distributed trait which is stable and significantly repeatable within males Froman and Feltmann, 2000). An artificial insemination experiment where the same number of sperm from a male with average-, and from a male with high-sperm mobility were mixed and artificially inseminated into the same female revealed that, when competing with low mobility ejaculates of the same size, high mobility ejaculates fertilised a disproportionate number of eggs (Birkhead et al, 1999).…”

Section: Post Insemination Strategies Malesmentioning

confidence: 99%

Pre- and post-insemination episodes of sexual selection in the fowl, Gallus g. domesticus

2002

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Although much attention has been recently directed to sexual selection arising after insemination from sexual promiscuity, little is known about the mechanisms determining reproductive success after insemination, and the way these mechanisms interact with each other and with selective mechanisms occurring before insemination: mate choice and mate acquisition. Here, we briefly review the findings of an on-going study investigating the mechanisms generating variation in reproductive success at both a pre-and a postinsemination stage in the domestic fowl. Female preference consistently favours socially dominant males before and

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Sperm Mobility: Phenotype in Roosters (Gallus domesticus) Determinedby Concentration of Motile Sperm and Straight Line Velocity1

Cited by 67 publications

References 9 publications

Short-term preservation of fowl sperm in buffered potassium chloride

Short-term preservation of fowl sperm in buffered potassium chloride

Sperm morphology, swimming velocity, and longevity in the house sparrow Passer domesticus

Pre- and post-insemination episodes of sexual selection in the fowl, Gallus g. domesticus

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