Sperm motility parameters forSteindachneridion parahybaebased on open-source software

Abstract

Summary The objective of this work was to evaluate the sperm motility of 13 Steindachneridion parahybae males using open‐source software (ImageJ/CASA plugin). The sperm activation procedure and image capture were initiated after semen collection. Four experimental phases were defined from the videos captured of each male as follows: (i) standardization of a dialogue box generated by the CASA plugin within ImageJ; (ii) frame numbers used to perform the analysis; (iii) post‐activation motility between 10 and 20 s with analysis at each 1 s; and (iv) post‐activation motility between 10 and 50 s with analysis at each 10 s. The settings used in the CASA dialogue box were satisfactory, and the results were consistent. These analyses should be performed using 50 frames immediately after sperm activation because spermatozoa quickly lose their vigor. At 10 s post‐activation, 89.1% motile sperm was observed with 107.2 μm s−1 curvilinear velocity, 83.6 μm s−1 average path velocity, 77.1 μm s−1 straight line velocity; 91.6% were of straightness and 77.1% of wobble. The CASA plugin within ImageJ can be applied in sperm analysis of the study species by using the established settings.

Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish

“…The sperm production found in this study was higher than the one verified for the same species by Caneppele et al (2015) and the computerized sperm characteristics were higher than the ones verified by Sanches et al (2013) for fresh semen, and by Araújo (2011) for cryopreserved semen. Therefore, the results obtained in this study were considered satisfactory for artificial reproduction carried out in laboratory routines.…”

“…Furthermore, the computerized analysis of sperm motility was conducted as proposed by Sanches et al (2010;2013).…”

“…The evaluation of sperm motility by the computerized method was carried out as described by Wilson-Leedy & Ingermann (2007) and adapted for neotropical catfishes by Sanches et al (2010;2013), using free software. The process of activation was conducted in Eppendorf tubes (1.5mL), using 1µL of semen and 1,000µL of water from the incubation system.…”

Seminal and spermatic characteristics of fresh semen and the effects of sperm cooling in Steindachneridion melanodermatum (Garavello, 2005)

“…Although there is little information regarding the reproductive biology of the genus Steindachneridion (HONJI et al, 2012), the sperm dynamics of S. parahybae have been analyzed (SANCHES et al, 2013). However, information about some seminal and sperm characteristics, including volume, viscosity, coloration, sperm concentration, and time of activation are available for S. melanodermatum based on subjective methods (SANT'ANNA, 2009).…”

Seminal and spermatic characteristics of fresh semen and the effects of sperm cooling in Steindachneridion melanodermatum (Garavello, 2005)

“…Sanches et al (2013) also determined the characteristics of MOT, VCL, VSL, and VAP in males of S. parahybae using computerized analysis. The results reported 10 s after sperm activation were lower than those verified in the present research for S. melanodermatum and showed values equal to 89.10%, 107.20 μm.s -1 , 77.10 μm.s -1 , and 83.60 μm.s -1 for MOT, VCL, VSL, and VAP, respectively.…”