Sperm nuclear DNA fragmentation in adolescents with varicocele

Bertolla, Ricardo Pimenta; Cedenho, A.P.; Filho, Pericles Assad Hassun; Lima, Samira Barbosa; Ortiz, Valdemar; Srougi, Miguel

doi:10.1016/j.fertnstert.2005.08.032

Cited by 105 publications

(73 citation statements)

References 23 publications

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“…Our data suggested that down-regulation of SOD1 in patients with varicocele and abnormal spermogram can elevate DNA fragmentation and apoptosis, but after varicocelectomy, SOD1 activity increases and it can be resulted in improving DNA damage. Several studies have demonstrated that the total seminal plasma antioxidant levels in patients with varicocele are decreased and DNA damage in these patients was increased [12][13][14][15][16][17][18]. Moreover, some studies have shown that varicocelectomy can improve DNA fragmentation and total antioxidant capacity [19,20].…”

Section: Discussionmentioning

confidence: 99%

Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis

Hosseinifar

Sabbaghian

Nasrabadi

et al. 2014

J Assist Reprod Genet

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Purpose To compare proteomic profiles of spermatozoa from patients with varicocele and poor sperm quality before and after varicocelectomy. Methods This work was designed as a prospective and observational study. The study was based on 20 men with varicocele grade 3 and poor sperm quality undergoing varicocelectomy at the Fertility Unit of Royan institute in 2009. Two semen samples were collected, one before varicocelectomy and the other after surgery. Protein separation was done by two-dimensional protein electrophoresis, and analyzed by gel densitometry and mass spectrometry. Differential sperm protein expression levels were measured by gel densitometry. Results Comparison of the sperm parameters showed that sperm motility and concentration were increased after varicocelectomy. At the level of protein, a total of 3 protein spots were identified whose expression was significantly lower in sperm samples before varicocelectomy compared with after surgery including heat shock protein A5 (HSPA5), superoxide dismutase 1 (SOD1) and δ-subunit of the catalytic core of mitochondrial adenosine triphosphate synthase (ATP5D). Conclusions High grade varicocoele affects sperm protein expression presumably because of increasing testicular temperature. These proteins play essential roles in sperm production, DNA integrity protection, and sperm motility. This novel study demonstrates that varicocelectomy can improve both sperm quality and proteins expression.

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Section: Discussionmentioning

confidence: 99%

Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis

Hosseinifar

Sabbaghian

Nasrabadi

et al. 2014

J Assist Reprod Genet

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“…We have also shown that, even when sperm concentration is not different, sperm DNA integrity is lower in adolescents with varicocele [57]. In this study, we did not set out to evaluate if varicocele does indeed cause a decrease in sperm concentration.…”

Section: Discussionmentioning

confidence: 85%

Expression of the Fas-ligand gene in ejaculated sperm from adolescents with and without varicocele

Giudice

Lima

Cenedeze

et al. 2010

J Assist Reprod Genet

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Purpose To assess FasL mRNA levels in ejaculated sperm from adolescent patients with and without varicocele. Methods Semen was obtained by masturbation following 2-4 days of ejaculatory abstinence, from 14 adolescents with varicocele grades II and III (study group), and 20 adolescents without varicocele (control group). Seminal analysis was done according to World Health Organization guidelines and morphology using Kruger's strict criteria. The Fas-ligand (FasL) gene expression was performed using reverse transcription and real-time quantitative polymerase chain reaction (RQ-PCR) analysis, according to the expression level of the housekeeping cyclophilin A gene. A Student's t-test was applied to compare the groups, and Spearman's rank test in order to verify possible correlations (p<0.05).Results Quantitative RQ-PCR demonstrated that the expression of FasL mRNA in sperm from the varicocele group was higher than in the control group. Also, sperm concentration was higher in the controls, when compared to the varicocele group. When submitted to correlation analysis, adolescents with varicocele presented a correlation between sperm concentration and FasL gene expression levels (r=−0.470), not observed in controls. Conclusion Our results allow us to conclude that, in adolescents with varicocele presenting lower sperm concentration, FasL mRNA levels are higher than in adolescents without varicocele.

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“…DNA integrity can be considered as a sperm function that is independent from sperm count, motility, and morphology [28,29]. Disturbances in sperm DNA integrity have been described in patients with varicoceles, testicular tumours, genital tract infections/inflammations, and patients with 'idiopathic' infertility [27,30,31].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Efficacy of Low Dose and High Dose Antioxidants along with Antibiotics in Idiopathic Oligo, Astheno and Oligoasthenospermia

Bm¹,

Ehsan²,

Ehsan³

et al. 2015

Andrology

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Objective: The aim of this study was to explore the efficacy of low dose and high dose antioxidants for the treatment of idiopathic oligiospermia, asthenospermia and oligoasthenospermia. Materials and methods:This prospective quasi experimental study was conducted in Infertility Care and Research Center (ICRC) Ltd, Dhaka, Bangladesh between January 2013 and December 2014. Eighty four patients were the target population for this study. After thorough investigations those patients were diagnosed as idiopathic oligo, astheno and oligoasthenospermia were recruited for this study. Those patients whose female partner had infertility factor except PCOS were excluded from this study. For treatment patients were divided into two groups by lottery. Treatment for group A was Cap Doxycycline 100 mg twice daily for 1 month and tablet Oligocare, low dose antioxidant (Meyer Organic Pvt Ltd, India) 1 tab twice daily for 2 months. Group B was treated by Cap doxycycline 100 mg twice daily for 1 month and combination of micronutrients (High dose antioxidants) for 2 months. Ovulation induction was given to female partner of patient whose semen parameters improved. Results for pregnancy were observed for 6 ovulatory cycles. Data were expressed as mean standard deviation and percentage. Student's t test and χ 2 tests were done for test of significance where appropriate. A p value <0.05 was considered as significant. Results:In both treatment groups both count and motility were increased significantly after 2 months of treatment. Though mean semen parameter improved significantly in both treatment groups, there was significant difference in number of patient improvement between the groups. In high dose group 79% patients improved after two months of treatment whereas 48% patients improved in low dose group. Similarly pregnancy rate was also higher in high dose group 22% in comparison to 12% in low dose group. Conclusion:Antioxidants if can be used at a higher dose instead of low dose can give better result in terms of improvement of sperm count, motility and subsequently pregnancy rates.

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Sperm nuclear DNA fragmentation in adolescents with varicocele

Cited by 105 publications

References 23 publications

Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis

Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis

Expression of the Fas-ligand gene in ejaculated sperm from adolescents with and without varicocele

Comparison of Efficacy of Low Dose and High Dose Antioxidants along with Antibiotics in Idiopathic Oligo, Astheno and Oligoasthenospermia

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