Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model

Mateo‐Otero, Yentel; Madrid-Gambin, Francisco; Llavanera, Marc; Gómez-Gómez, Álex; Haro, Noemí; Pozo, Óscar J.; Yeste, Marc

doi:10.1038/s42003-023-04715-3

Cited by 12 publications

(2 citation statements)

References 63 publications

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“…This effect was not as evident as seen in recent findings in pig spermatozoa in which the addition of itaconate improved sperm motility, 24 a species difference that may be related to the different metabolic strategies of the spermatozoa 57 and the varying composition of the media used in the studies. The study in pigs used 40 mM glucose.…”

Section: Discussioncontrasting

confidence: 71%

“…While stallion spermatozoa are highly dependent on OXPHOS for the generation of ATP, porcine spermatozoa seem to have a different metabolic strategy in which OXPHOS is present, but glycolysis predominates. 57,58 In summary, we show that stallion spermatozoa can be stored for up to 96 h at 22 • C in a 67 mM glucose-10 mM pyruvate-based media.…”

Section: Discussionmentioning

confidence: 70%

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Aging of stallion spermatozoa stored in vitro is delayed at 22°C using a 67 mm glucose–10 mm pyruvate‐based media

Becerro‐Rey,

Martín‐Cano,

Ferrusola

et al. 2023

Andrology

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BackgroundMost commerce of equine seminal doses is carried out using commercial extenders under refrigeration at 5°C.ObjectivesTo determine if 10 mm pyruvate in a 67 mm glucose extender and storage at 22°C could be the basis of an alternative storage method to cooling to 5°C.Material and methodsStallion ejaculates were extendedin: INRA96 (67 mm glucose, non‐pyruvate control), modified Tyrode's (67 mm glucose–10 mm pyruvate), supplemented with 0, 10, 50, and 100 μM itaconate. As itaconate was vehiculated in DMSO, a control vehicle was also included. Sperm motility, viability, mitochondrial membrane potential, and production of reactive oxygen species were measured after collection and again after 48 and 96 h of storage at 22°C. To disclose molecular metabolic changes, spermatozoa were incubated up to 3 h in modified Tyrode's 67 mm glucose–10 mm pyruvate and modified Tyrode's 67 mm glucose, and metabolic analysis conducted.ResultsAfter 96 h of storage aliquots stored in the control, INRA96 had a very poor total motility of 5.6% ± 2.3%, while in the 67 mm glucose–10 mm pyruvate/10 μm itaconate extender, total motility was 34.7% ± 3.8% (p = 0.0066). After 96 h, viability was better in most pyruvate‐based media, and the mitochondrial membrane potential in spermatozoa extended in INRA96 was relatively lower (p < 0.0001). Metabolomics revealed that in the spermatozoa incubated in the high pyruvate media, there was an increase in the relative amounts of NAD+, pyruvate, lactate, and ATP.Discussion and conclusionsAliquots stored in a 67 mm glucose–10 mm pyruvate‐based medium supplemented with 10 μM itaconate, maintained a 35% total motility after 96 h of storage at 22°C, which is considered the minimum acceptable motility for commercialization. Improvements may be related to the conversion of pyruvate to lactate and regeneration of NAD+.

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Section: Discussioncontrasting

confidence: 71%

Section: Discussionmentioning

confidence: 70%

Aging of stallion spermatozoa stored in vitro is delayed at 22°C using a 67 mm glucose–10 mm pyruvate‐based media

Becerro‐Rey,

Martín‐Cano,

Ferrusola

et al. 2023

Andrology

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Sperm Metabolism

Abruzzese,

Sanchez‐Rodriguez,

Roldan

2024

Molecular Reproduction Devel

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Bioenergetics plays a crucial role in sperm functions, including motility, capacitation‐related protein modifications, oocyte recognition and interaction, all of which are essential for fertilization. Sperm metabolism is recognized as flexible, responding to environmental cues and energetic demands during ejaculation, the journey along the female tract, and until fertilization. Recent studies suggest that sperm metabolic functions are relevant beyond fertilization and may influence zygote and embryo development, impacting paternal‐derived effects on offspring development and health. In recent years, sperm metabolic functions and homeostasis have gained increasing interest in male reproduction research. Given the crucial implications of sperm metabolism on fertility‐related processes, this field is of interest not only in human male fertility but also in livestock research, semen conservation, and assisted reproductive techniques. Newly developed assessment tools are allowing a better understanding of sperm metabolism under different conditions and identifying species‐specific peculiarities. This review aims to discuss the current knowledge of mammalian sperm metabolism, focusing on species‐specific features, changes during the sperm journey, and potential contributions to translational research and reproductive biotechnologies. Furthermore, we propose future perspectives on sperm bioenergetics research.

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Artificial intelligence and porcine breeding

García-Vázquez

2024

Animal Reproduction Science

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Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model

Cited by 12 publications

References 63 publications

Aging of stallion spermatozoa stored in vitro is delayed at 22°C using a 67 mm glucose–10 mm pyruvate‐based media

Aging of stallion spermatozoa stored in vitro is delayed at 22°C using a 67 mm glucose–10 mm pyruvate‐based media

Sperm Metabolism

Artificial intelligence and porcine breeding

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