Sperm Preparation Methods

MORTIMER, DAVID

doi:10.1002/j.1939-4640.2000.tb03390.x

Cited by 120 publications

(3 citation statements)

References 61 publications

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“…It is generally known that improper centrifugation speed can lead to poor sperm recovery. 23 Fourth , centrifugation was carried out in test tubes without using a density gradient, which is a better way to push the responsible cells to the tube’s bottom. 22 In addition, if no density gradient was used, big chunks of rough tissue would fall toward the bottom along with the cells of interest.…”

Section: Discussionmentioning

confidence: 99%

Staged Laboratory Processing of Testicular Tissue in Non-Obstructive Azoospermia May Rescue Retrieving an Existing Sperm: A Case Report and Literature Review

Salama

Hassan

2023

Clin Med Insights Case Rep

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Non-obstructive azoospermia (NOA) is the most difficult form of male infertility to manage. It usually requires sperm retrieval from the testis, which is most challenging due to sperm rarity. Here, we describe the recovery of testicular sperms that had been missing and whose original retrieval results were negative. Salvage microsurgical testicular sperm extraction and sperm testing were performed on a 36-year-old male with NOA. Neither in the operation room nor after an inspection in the embryology laboratory were any sperm detected. The obtained tissue was advised to be frozen because the patient data and surgical microscopy predicted a favorable outcome, and the tissue processing was done in an inappropriate environment. About 1 month later, the specimen was thawed, crushed, and re-examined. Successful oocyte fertilization resulted from an effective detection of sperms and their direction to intra-cytoplasmic sperm injection. This is the first case report that, to the best of our knowledge, describes the stepwise laboratory processing of testicular tissue and its capacity to recover lost sperms in challenging NOA cases and under less-than-ideal working conditions.

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Section: Discussionmentioning

confidence: 99%

Staged Laboratory Processing of Testicular Tissue in Non-Obstructive Azoospermia May Rescue Retrieving an Existing Sperm: A Case Report and Literature Review

Salama

Hassan

2023

Clin Med Insights Case Rep

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“…In clinical decision making, as well as the testing and evaluation of sperm preparation devices, significant attention is often paid to yield (i.e., the number of sperm in the prepared sample) 62 : in particular, the decision of whether to progress a couple to ICSI rather than IVF treatment, with the latter requiring sufficient progressively motile sperm. There are questions regarding whether this is the right approach.…”

Section: Before Selection-optimize the Populationmentioning

confidence: 99%

Only the Best of the Bunch—Sperm Preparation Is Not Just about Numbers

Gallagher,

Krasauskaite,

Kirkman-Brown

2023

Semin Reprod Med

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In this Seminar, we present an overview of the current and emerging methods and technologies for optimizing the man and the sperm sample for fertility treatment. We argue that sperms are the secret to success, and that there are many avenues for improving both treatment and basic understanding of their role in outcomes. These outcomes encompass not just whether treatment is successful or not, but the wider intergenerational health of the offspring. We discuss outstanding challenges and opportunities of new technologies such as microfluidics and artificial intelligence, including potential pitfalls and advantages. This article aims to provide a comprehensive overview of the importance of sperm in fertility treatment and suggests future directions for research and innovation.

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“…Then the samples were processed by density gradient and swim-up method (Mortimer, 2000) to analyze the sperm motility and concentration after capacitation. Later, the post-capacitated semen was used for ICSI and fertilization rates were evaluated in the two groups.…”

Section: Study Groupsmentioning

confidence: 99%

Oligoasthenozoospermic and Normozoospermic Indian Males Yielded Similar Fertilization Capability by Intracytoplasmic Sperm Injection

Dayal

2023

JMRH

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Background: The present study evaluated the fertilization outcomes by intra-cytoplasmic sperm injection (ICSI) technique with oligoastheno-zoospermic (OAZ) compared to normozoospermic (NZ) semen. Purpose: To find the fertilizing capability of human sperm in oligo-asthenozoospermic and normozoospermic semen samples. Methods: Fresh semen ejaculates belonging to NZ and OAZ groups (n=50 in each group) patients attending the infertility clinics were evalu-ated for semen volume, sperm motility, and sperm concentration. Ferti-lization rates with ICSI along with post-capacitation motility and con-centration were studied in both groups. Results: The motility and concentration in fresh semen samples patients of the NZ group (32.44±1.50%, 71.96±6.45 M/ml, respectively) were significantly (P<0.05) higher compared to the OAZ group (26.76±1.38, 10.31±0.48 M/ml, respectively).However, the results of our study re-vealed similar fertilization rates in-vitro by ICSI in the NZ (98%) and OAZ (98%) groups. Conclusion:The in-vitro processing by the ICSI technique is an effective approach to achieving good fertilization rates in sub-fertile males.

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Sperm Preparation Methods

Cited by 120 publications

References 61 publications

Staged Laboratory Processing of Testicular Tissue in Non-Obstructive Azoospermia May Rescue Retrieving an Existing Sperm: A Case Report and Literature Review

Staged Laboratory Processing of Testicular Tissue in Non-Obstructive Azoospermia May Rescue Retrieving an Existing Sperm: A Case Report and Literature Review

Only the Best of the Bunch—Sperm Preparation Is Not Just about Numbers

Oligoasthenozoospermic and Normozoospermic Indian Males Yielded Similar Fertilization Capability by Intracytoplasmic Sperm Injection

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