Sperm protein 17 expression defines 2 subsets of primary esthesioneuroblastoma

Bumm, Klaus; Grizzi, Fabio; Franceschini, Barbara; Koch, Michael; Iro, Heinrich; Wurm, Jochen; Ceva-Grimaldi, Giorgia; Dimmler, Arno; Cobos, Everado; Dioguardi, N; Sinha, Uttam K.; Kast, W. Martin; Chiriva‐Internati, Maurizio

doi:10.1016/j.humpath.2005.08.023

Cited by 19 publications

(13 citation statements)

References 24 publications

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“…However, our analyses predicted that Spa17 is present in olfactory cilia. Spa17 has previously been localized to sperm flagella and cilia of the respiratory tract and reproductive organs and is known to be expressed by OSNs, but it has not been localized to the cilia of OSNs (6,12,16). Not surprisingly, we observed that Spa17 immunoreactivity in the olfactory epithelium overlaps completely with immunoreactivity for acetylated tubulin, a marker for cilia (Fig.…”

Section: Resultsmentioning

confidence: 99%

Tissue expression patterns identify mouse cilia genes

McClintock

Glasser

Bose

et al. 2008

Physiological Genomics

128

124

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In mammals, cilia are critical for development, sensation, cell signaling, sperm motility, and fluid movement. Defects in cilia are causes of several congenital syndromes, providing additional reasons to identify cilia-related genes. We hypothesized that mRNAs selectively abundant in tissues rich in highly ciliated cells encode cilia proteins. Selective abundance in olfactory epithelium, testes, vomeronasal organ, trachea, and lung proved to be an expression pattern uniquely effective in identifying documented cilia-related genes. Known and suspected cilia-related genes were statistically overrepresented among the 99 genes identified, but the majority encoded proteins of unknown function, thereby predicting new cilia-related proteins. Evidence of expression in a highly ciliated cell, the olfactory sensory neuron, exists for 73 of the genes. In situ hybridization for 17 mRNAs confirmed expression of all 17 in olfactory sensory neurons. Most were also detected in vomeronasal sensory neurons and in neighboring tissues rich in ciliated cells such as respiratory epithelium. Immunoreactivity for one of the proteins identified, Spa17, colocalized with acetylated tubulin in the cilia layer of the olfactory epithelium. In contrast, the ciliary rootlet protein, Crocc, was located in discrete structures whose position was consistent with the dendritic knobs of the olfactory sensory neurons. A compilation of >2,000 mouse genes predicted to encode cilia-related proteins revealed a strong correlation (R = 0.99) between the number of studies predicting a gene's involvement in cilia and documented evidence of such involvement, a fact that simplifies the selection of genes for further study of the physiology of cilia.

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Section: Resultsmentioning

confidence: 99%

Tissue expression patterns identify mouse cilia genes

McClintock

Glasser

Bose

et al. 2008

Physiological Genomics

128

124

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“…Nakazato et al identified Sp17 as a candidate gene related to the chemoresistance of clear cell carcinoma [21]. Bumm et al showed that Sp17 can be used as a marker to discriminate between 2 subsets of primary esthesioneuroblastomas [9]. …”

Section: Discussionmentioning

confidence: 99%

“…In addition to testis and ejaculated sperm, HSp17 was also detected in ciliated epithelia of the respiratory airways and both the male and female reproductive systems [5]. Recent evidence suggested that HSp17 was aberrantly expressed in many malignant tumors, such as multiple myelomas [6], ovarian cancers [7], esophageal squamous cell carcinomas [8], esthesioneuroblastomas [9], and various histological subtypes of human nervous system (NS) malignancies, including neuroectodermal and meningeal tumors [10]. Wang et al .…”

Section: Introductionmentioning

confidence: 99%

Overexpression of human sperm protein 17 increases migration and decreases the chemosensitivity of human epithelial ovarian cancer cells

et al. 2009

BMC Cancer

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BackgroundMost deaths from ovarian cancer are due to metastases that are resistant to conventional therapies. But the factors that regulate the metastatic process and chemoresistance of ovarian cancer are poorly understood. In the current study, we investigated the aberrant expression of human sperm protein 17 (HSp17) in human epithelial ovarian cancer cells and tried to analyze its influences on the cell behaviors like migration and chemoresistance.MethodsImmunohistochemistry and immunocytochemistry were used to identify HSp17 in paraffin embedded ovarian malignant tumor specimens and peritoneal metastatic malignant cells. Then we examined the effect of HSp17 overexpression on the proliferation, migration, and chemoresistance of ovarian cancer cells to carboplatin and cisplatin in a human ovarian carcinoma cell line, HO8910.ResultsWe found that HSp17 was aberrantly expressed in 43% (30/70) of the patients with primary epithelial ovarian carcinomas, and in all of the metastatic cancer cells of ascites from 8 patients. The Sp17 expression was also detected in the metastatic lesions the same as in ovarian lesions. None of the 7 non-epithelial tumors primarily developed in the ovaries was immunopositive for HSp17. Overexpression of HSp17 increased the migration but decreased the chemosensitivity of ovarian carcinoma cells to carboplatin and cisplatin.ConclusionHSp17 is aberrantly expressed in a significant proportion of epithelial ovarian carcinomas. Our results strongly suggest that HSp17 plays a role in metastatic disease and resistance of epithelial ovarian carcinoma to chemotherapy.

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“…The results of this study indicated the usefulness of SP17 as a means of discriminating two subsets of primary tumor lesions [62].…”

Section:  M Chiriva-internatimentioning

confidence: 59%

Sperm Protein 17: Clinical Relevance of a Cancer/Testis Antigen, from Contraception to Cancer Immunotherapy, and Beyond

Chiriva‐Internati

2011

International Reviews of Immunology

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Sperm protein 17 belongs to the cancer/testis antigen family and was found to play a key role in the cell fusion process between the mammalian spermatozoa and egg. Sperm protein 17-specific autoantibodies in vasectomized males suggest its high expression in the testis. Sperm protein 17 expression levels are low or absent in normal tissues, other than the testis. Sperm protein 17 is expressed by tumors, including ovarian cancer, indicating that it is an ideal target for cancer immunotherapy, and plays a role in tumorigenesis and drug resistance. This review recapitulates the milestones of sperm protein 17 research and highlights its potential use in translational medicine.

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Sperm protein 17 expression defines 2 subsets of primary esthesioneuroblastoma

Cited by 19 publications

References 24 publications

Tissue expression patterns identify mouse cilia genes

Tissue expression patterns identify mouse cilia genes

Overexpression of human sperm protein 17 increases migration and decreases the chemosensitivity of human epithelial ovarian cancer cells

Sperm Protein 17: Clinical Relevance of a Cancer/Testis Antigen, from Contraception to Cancer Immunotherapy, and Beyond

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