Sperm quality and pregnancy rate after COX-2 inhibitor therapy of infertile males with abacterial leukocytospermia

Gambera, Laura; Serafini, Francesca; Morgante, Giuseppe; Focarelli, Riccardo; Leo, Vincenzo De; Piomboni, Paola

doi:10.1093/humrep/del490

Cited by 65 publications

(53 citation statements)

References 23 publications

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“…It has been demonstrated that inhibitors of COX-2 have a positive effect on sperm quality, associated with an increase in the volume of the ejaculate in humans (Gambera et al, 2007). This would be useful in further studies to evaluate the security of non-steroidal anti-inflammatory drugs in the testes of animals exposed to boron.…”

Section: Morphometric and Histopathological Analysismentioning

confidence: 96%

Boron as Testicular Toxicant in Mice (Mus domesticus)

Bustos‐Obregón

Olivares

2012

Int. J. Morphol.

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SUMMARY:It has been reported that boron causes changes in various systems, including the male reproductive system. Residents in some towns in northern Chile were consuming a few years ago in the drinking water 20 times more than the amount established as permissible limit by WHO. This study evaluates the effects in an animal model of high intake of boron on the testis. Boron was administered in the drinking water. Twenty male mice (Mus domesticus), sexually mature, were used, divided into 2 groups: the experimental group was given Boron at a dose of 12 mg/L, and the control group 0.6 mg/L, for 42 days. Sections of testis were obtained for: HE staining (Morphometry and Histopathology), Immunohistochemistry (Cox-2), Mallory and Picrosirious stain (evaluation of tunica albuginea). The results indicate that ingestion of a dose of 12 mg Boron/L produces vacuolization, tubular epithelial desquamation and tamponade. Morphometry revealed decreased tubular diameter and epithelial height and lumen diameter and increased interstitial area in the exposed group. Immunodetection of COX-2 was positive in high percentage of tubules in the intoxicated group. The tunica albuginea was thinner, with decreased percentage of type I collagen fibers and an increase in the percentage of type III collagen fibers in animals exposed to boron in contrast to the control group. Exposure to critical levels of boron produces severe histopathological changes in the testis, altering morphometric parameters and causing overexpression of Cox-2. Finally, evaluation of collagen fibers suggests that Boron produced a degradation of the collagen of the tunica albuginea, causing a decrease in the thickness of it and altering the percentage ratio collagen I/collagen III, a process called collagenolysis.

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Section: Morphometric and Histopathological Analysismentioning

confidence: 96%

Boron as Testicular Toxicant in Mice (Mus domesticus)

Bustos‐Obregón

Olivares

2012

Int. J. Morphol.

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“…In one study men with antibiotic treated Chlamydia or Ureaplasma infection were randomized to either a NSAID or carnitine antioxidant and monitored for improvements in sperm quality over the next 4 months [151]. In addition, a one month course of a COX-2 anti-inflammatory along with 2 months of carnitine has been shown to significantly reduce sperm leukocyte count, while improving sperm motility, morphology and viability [152].…”

Section: Treating Infectionsmentioning

confidence: 99%

Supraphysiological Free Radical Levels and their Pathogenesis in Male Infertility

Dinesh¹

2012

Reprod Sys Sexual Disorders

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Oxidative stress is an important aetiological factor which leads to sperm DNA damage and infertility. It damages all biomolecules and both mitochondrial and nuclear DNA and adversely affects sperm membrane fluidity and motility. This acts like a biological safeguard however use of such sperm for ART/ICSI can lead to pre and post implantation losses, major and minor congenital malformations and even childhood cancer. Thus it is important to know the causes of oxidative stress and how the levels of free radicals be maintained at physiological levels when they are beneficial for normal function. It is also important to develop techniques to identity cases with high free radical levels and also adopt certain life style measures which can minimise oxidative stress and improve male reproductive health.

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“…The demonstration of an association between increased seminal leukocyte concentrations and decreased male fertility has led to the investigation of therapeutic strategies for leukocytospermia (5,11,16,17). Studies have shown that reducing leukocyte concentrations can help improve other semen parameters, such as sperm concentration, motility, and morphology (11,16,17).…”

mentioning

confidence: 99%

“…Studies have shown that reducing leukocyte concentrations can help improve other semen parameters, such as sperm concentration, motility, and morphology (11,16,17). However, most of these studies were not placebo controlled and therefore did not provide data on spontaneous improvement of, or remission rates for, leukocytospermia in the absence of treatment.…”

mentioning

confidence: 99%