Sperm quality assessment in Ficopomatus enigmaticus (Fauvel, 1923): Effects of selected organic and inorganic chemicals across salinity levels

Cuccaro, Alessia; Marchi, Lucia De; Oliva, Matteo; Sanches, Matilde Vieira; Freitas, Rosa; Casu, Valentina; Monni, Gianfranca; Miragliotta, Vincenzo; Pretti, Carlo

doi:10.1016/j.ecoenv.2020.111219

Cited by 19 publications

(4 citation statements)

References 41 publications

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“…Previous studies also frequently used other different biomarkers, like oxidative stress, membrane integrity, and 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) measurements to indirectly assess the sperm quality and reproductive toxicity induced by chemicals (Cuccaro et al, 2021;De Marchi et al, 2020). Related studies also establish some molecular indicators, like the earthworm annetocin gene that shows significant down-regulation after Cd exposure, and in turn influencing the J o u r n a l P r e -p r o o f 20 cocoon production processes (Mo et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Reproductive damage and compensation of wild earthworm Metaphire californica from contaminated fields with long-term heavy metal exposure

Huang

Shen

et al. 2023

Chemosphere

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Section: Discussionmentioning

confidence: 99%

Reproductive damage and compensation of wild earthworm Metaphire californica from contaminated fields with long-term heavy metal exposure

Huang

Shen

et al. 2023

Chemosphere

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“…A battery of sperm quality biomarkers (sperm vitality (MTT), lipid peroxidation (LPO), intracellular reactive oxygen species (ROS), and DNA damage) was adopted to assess the spermiotoxic effect of all tested extracts at all concentrations in F. enigmaticus , according to Cuccaro et al ( 2020 ). Ten-fold concentrated stock solutions were prepared in ASW for each tested concentration (see the “Preparation of extracts” section).…”

Section: Methodsmentioning

confidence: 99%

“…Analyses were performed adding 100 μL of extract to 1000 μL of sperm suspension for each concentration, which was incubated with for 30 min at room temperature (RT). After the exposure, the solutions were transferred on a 96-well plate for sperm quality assessment (Cuccaro et al 2020 ).…”

Section: Methodsmentioning

confidence: 99%

A multi-bioassay integrated approach to assess antifouling potential of extracts from the Mediterranean sponge Ircinia oros

Marchi

Pretti

Cuccaro

et al. 2021

Environ Sci Pollut Res

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The phylum Porifera and their symbionts produce a wide variety of bioactive compounds, playing a central role in their ecology and evolution. In this study, four different extracts (obtained by non-polar and semi-polar extraction methodologies) of the Mediterranean sponge Ircinia oros were tested through a multi-bioassay integrated approach to assess their antifouling potential. Tests were performed using three common species, associated with three different endpoints: the marine bacterium Aliivibrio fischeri (inhibition of bioluminescence), the marine diatom Phaeodactylum tricornutum (inhibition of growth), and different development stages of the brackish water serpulid Ficopomatus enigmaticus (gametes: sperm motion, vitality inhibition and cellular damage; larvae: development; adults: AChE (acetylcholinesterase)-inhibitory activity). The effects of extracts were species specific and did not vary among different extraction methodologies. In particular, no significant reduction of bioluminescence of A. fischeri was observed for all tested samples. By contrast, extracts inhibited P. tricornutum growth and had toxic effects on different F. enigmaticus’ developmental stages. Our results suggest that the proposed test battery can be considered a suitable tool as bioactivity screening of marine natural products.

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“…A battery of sperm quality biomarkers (sperm vitality (MTT), lipid peroxidation (LPO), intracellular reactive oxygen species (ROS) and DNA damage) was adopted to assess the spermiotoxic effect of all tested extracts at all concentrations in F. enigmaticus, according to Cuccaro et al (2020). Tenfold concentrated stock solutions were prepared in ASW for each tested concentration (see Sect.…”

Section: Gametesmentioning

confidence: 99%

Potential Anti-Fouling Properties of Extracts from the Mediterranean Sponge Ircinia Oros: An Ecotoxicological Screening

Marchi

Pretti

Cuccaro

et al. 2021

Preprint

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The phylum Porifera and their symbionts produce a wide variety of bioactive compounds, playing a central role in their ecology and evolution. In this study, four different extracts (obtained by non-polar and semi-polar extraction methodologies) from the Mediterranean sponge Ircinia oros were tested for their potential antifouling purposes. The evaluation was performed using three different target species, associated with three different endpoints: the marine bacterium Aliivibrio fischeri (inhibition of bioluminescence), the marine diatom Phaeodactylum tricornutum (inhibition of growth) and different development stages of the brackish-water serpulid Ficopomatus enigmaticus (gametes: sperm motion, vitality inhibition and cellular damage; larvae: development; adults: AChE (acetylcholinesterase)-inhibitory activity). Effects of extracts were species-specific and did not vary among different extraction methodologies. In particular, no significant reduction of bioluminescence of A. fischeri was observed for all tested samples. By contrast, extracts inhibited P. tricornutum growth and had toxic effects on different F. enigmaticus’ developmental stages. Obtained results suggest that the proposed test battery can be considered a suitable tool as bioactivity screening of marine natural products.

show abstract

Sperm quality assessment in Ficopomatus enigmaticus (Fauvel, 1923): Effects of selected organic and inorganic chemicals across salinity levels

Cited by 19 publications

References 41 publications

Reproductive damage and compensation of wild earthworm Metaphire californica from contaminated fields with long-term heavy metal exposure

Reproductive damage and compensation of wild earthworm Metaphire californica from contaminated fields with long-term heavy metal exposure

A multi-bioassay integrated approach to assess antifouling potential of extracts from the Mediterranean sponge Ircinia oros

Potential Anti-Fouling Properties of Extracts from the Mediterranean Sponge Ircinia Oros: An Ecotoxicological Screening

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