1985
DOI: 10.1002/aja.1001740306
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Sperm surface changes during the acrosome reaction as observed by freeze‐fracture

Abstract: The mammalian acrosome reaction is an exocytotic process that can be analyzed by the technique of freeze-fracture; only sperm cells capacitated in vitro or treated to elicit the acrosome reaction in vitro have been studied, and all pictures published are from material fixed before freezing. All the authors point out the appearance of particle-free areas in the plasma membrane of the acrosomal region during capacitation and before any fusion. This is interpreted as an increase in membrane fluidity as suggested … Show more

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Cited by 29 publications
(10 citation statements)
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“…Arrays of IMPs were observed in the PM overlying the AR of ram spermatozoa ), the acrosome of monkey (Reger et al 1985) and rat spermatozoa (Toyama and Nagano 1988). A localized ordered distribution of IMP was also found in several species in the OAM (Friend and Fawcett 1974;Yanagimachi and Suzuki 1985;Aguas and Pinto Da Silva 1989) and in the IAM (Koehler 1975;Huang and Yanagimachi 1985;Fléchon 1985;Olson and Winfrey 1985a).…”
Section: Plasma and Acrosomal Membranes: Fusion Problematicsmentioning
confidence: 86%
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“…Arrays of IMPs were observed in the PM overlying the AR of ram spermatozoa ), the acrosome of monkey (Reger et al 1985) and rat spermatozoa (Toyama and Nagano 1988). A localized ordered distribution of IMP was also found in several species in the OAM (Friend and Fawcett 1974;Yanagimachi and Suzuki 1985;Aguas and Pinto Da Silva 1989) and in the IAM (Koehler 1975;Huang and Yanagimachi 1985;Fléchon 1985;Olson and Winfrey 1985a).…”
Section: Plasma and Acrosomal Membranes: Fusion Problematicsmentioning
confidence: 86%
“…In the guinea pig spermatozoa, the induced fusion starts in the huge anterior segment and progresses across the principal segment, along the branching arrays of hybrid membrane tubules, as revealed by transmission electron microscopy (Flaherty and Olson 1991). The Ca2+-ionophore treatment of ram spermatozoa initiates the acrosome reaction at the posterior limit of the AS as observed after freeze-fracture (Fléchon 1985); fusion proceeds towards the AR as branching tubules following hexagonal patterns similar to that of the IMPs in the IAM. Ionophore-induced membrane fusion in the boar spermatozoa starts in the same place and progresses in the same direction as shown by the electron-micrographs of Topfer-Pedersen et al (1985).…”
Section: Plasma and Acrosomal Membranes: Fusion Problematicsmentioning
confidence: 92%
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“…The last could be associated with capricious and fluctuating localization of the HS 1A.1 antigen patches over the sperm head. The sperm membrane destabilization culminates in the acrosome reaction, for which an extensive rearrangement of intramembranous particles, especially in the plasma membrane, has been reported as an early event (Jamil et ai, 1982;Fléchon, 1985;Fléchon et ai, 1986), probably related to increased plasma membrane fluidity (Bearer & Friend, 1982;Friend, 1984). During Ca2 + /A23187 treatment a part of the HS 1A.1 antigen migrates on a time scale of only a few minutes, i.e.…”
Section: Discussionmentioning
confidence: 97%
“…A few freeze-fracture studies of abalone (29) and mammalian sperm (9,10,15,40) have demonstrated the regionalization of intramembrane particles (IMPs) associated with the acrosome reaction, while little information is available concerning the distribution of sterols in the sperm membrane during the acrosome reaction (12). Sterol is an important component of the cell membrane and plays a fundamental role in modulating the permeability and fluidity of membranes (6,24,33).…”
mentioning
confidence: 99%