Sperm Survival: Relationship to Age-Related Sperm DNA Integrity in Infertile Men

Abstract

The purpose of our study was to evaluate the effect of age and sperm DNA integrity on sperm survival. Semen samples from fifty six unselected patients undergoing infertility evaluation were assessed in terms of standard semen parameters, DNA integrity, and sperm survival after 6-24 h of incubation. Prolonged incubation of density gradient selected sperm adversely effects sperm survival in older patients and patients with extensive sperm DNA damage. Immediate preparation of such samples prior to use for assisted reproductive technology (ART) may overcome the negative effect of extended incubation.

Association of sperm apoptosis and DNA ploidy with sperm chromatin quality in human spermatozoa

“…The mature sperm fraction prepared by density-gradient centrifugation has improved longevity and sperm DNA integrity (53), although a recent report recommended immediate semen preparation before use for ART to overcome the negative effect of extended (6-24 hour) incubation (54). The aim of this study was to evaluate the association of sperm chromatin integrity and maturity with sperm apoptotic stages and the apoptotic subpopulation in unprocessed as well as mature and immature sperm fractions processed by density-gradient separation.…”

Effects of men and recipients’ age on the reproductive outcome of an oocyte donation program

“…Recent studies have demonstrated an increase in sperm DNA damage with age in healthy [8,31,32] and infertile men [33][34][35]. DNA fragmentation has been proposed as one of the reasons for the negative paternal effects on reproductive outcome, because a high percentage of DNA fragmentation has been correlated with low pregnancy rate [36].…”

Correlation between male age, WHO sperm parameters, DNA fragmentation, chromatin packaging and outcome in assisted reproduction technology

“…However, no association could be determined with an increase in aneuploidy/diploidy or specific FGFR2 mutations. An increase in DNA fragmentation (demonstrated by TUNEL assay and SCSA) and poor chromatin packaging (by CMA 3 staining) has been linked to OAT patients with increased age (Moskovtsev et al. , 2006, 2007; Plastira et al.…”