Sperm ultrastructure of five species of the Neotropical ant genus <i>Pseudomyrmex</i> (Hymenoptera: Formicidae)

Moya, J.; Mancini, Karina Carvalho; Lino‐Neto, José; Delabie, Jacques Hubert Charles; Dolder, Heidi

doi:10.1111/j.1463-6395.2007.00264.x

Cited by 18 publications

(21 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mitochondrial derivatives symmetric in diameter, as observed in L. dieloceri, is a characteristic shared to other Ichneumonidae (Quicke et al, 1992), Chalcidoidea (Quicke et al, 1992;Lino-Neto et al, 1999, 2000aLino-Neto and Dolder, 2001b;Brito et al, 2009) and some ants (Wheeler et al, 1990;Wheeler and Krutzsch, 1992;Lino-Neto and Dolder, 2002;Moya et al, 2007). In Pachysomoides sp.…”

Section: Discussionmentioning

confidence: 86%

“…Spermatozoa from L. dieloceri, measuring 140 mm, are the longest reported for Ichneumonidae; while spermatozoa from Pachysomoides sp., measuring 28 mm, are the smallest registered for the family, with observations varying from 55 mm (Alomyinae, Quicke et al, 1992) to 100 mm (Ichneumoninae, Chauvin et al, 1988) in literature. Spermatozoa from the two species analyzed are linear as found in other species of Ichneumonidae (Quicke et al, 1992) and in most Aculeata (Hoage and Kessel, 1968;Cruz-Hö fling et al, 1970;Lensky et al, 1979;Wheeler et al, 1990;Peng et al, 1992Peng et al, , 1993Lino-Neto and Dolder, 2002;Zama et al, 2001Zama et al, , 2004Zama et al, , 2005aAraú jo et al, 2005bAraú jo et al, , 2009Fiorillo et al, 2005;Mancini et al, 2006;Moya et al, 2007). However, linear spermatozoa differentiate Ichneumonidae from other parasitic wasps such as Chalcidoidea (Lino-Neto et al, 1999, 2000aBrito et al, 2009) andPlatygastroidea (Lino-Neto andDolder, 2001b), which present spiral spermatozoa.…”

Section: Discussionmentioning

confidence: 91%

“…However, in parasites from the superfamily Chalcidoidea (Lino-Neto et al, 2000a, 2001b, in Symphyta Tremex sp. (Siricoidea) (Newman and Quicke, 1999), in Crabroninae (Araú jo et al, 2009) and in ants (Moya et al, 2007), the centriolar adjunct is located frontally to the two mitochondrial derivatives and is classified as symmetric.…”

Section: Discussionmentioning

confidence: 98%

See 2 more Smart Citations

Structural and ultrastructural characteristics of male reproductive tract and spermatozoa in two Cryptinae species (Hymenoptera: Ichneumonidae)

Moreira

Araújo

Báo

et al. 2010

Micron

View full text Add to dashboard Cite

This study represents the first characterization of male reproductive tracts and ultrastructural description of sperm of Cryptinae species. In Lymeon dieloceri and Pachysomoides sp., the male reproductive tract is formed by a pair of testis, two deferent ducts, two accessory glands and one ejaculatory duct. The spermatozoa are similar to those described for other Hymenoptera, with: (1) the acrosome formed by the acrosomal vesicle covering the perforatorium, which has its base inserted in a cavity located in the nucleus point; (2) a thin nucleus with electron-dense chromatin; (3) an electron-dense centriolar adjunct located between the nucleus and one of the two mitochondrial derivatives; (4) an axoneme with a 9 + 9 + 2 microtubule arrangement; (5) two long mitochondrial derivatives with peripheral cristae and; (6) two accessory bodies located between the two mitochondrial derivatives and the axoneme. These ichneumonids present structural characteristics similar to other parasitic wasps, such as presence of a single follicle per testis, layer of extracellular material enveloping the acrosome and accessory microtubules ending before others in final portion of the flagellum. However, male reproductive system and the spermatozoa presented morphological characteristics that allowed their differentiation, such as oval shapes accessory glands and the symmetric mitochondrial derivatives in L. dieloceri compared to the spherical accessory glands and asymmetrical derivatives observed in Pachysomoides sp. Taken together, data presented here demonstrates that diversity of morphological characteristic from the male reproductive tract and spermatozoa in Hymenoptera might provide a character system that can be used, in association with other systems, to resolve various uncertainties about the evolutionary relationships of this insect group.

show abstract

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 91%

See 1 more Smart Citation

Structural and ultrastructural characteristics of male reproductive tract and spermatozoa in two Cryptinae species (Hymenoptera: Ichneumonidae)

Moreira

Araújo

Báo

et al. 2010

Micron

View full text Add to dashboard Cite

show abstract

“…In some Apidae the chromatin is decompressed throughout the nuclear area (Zama et al, 2004;Fiorillo et al, 2005;Gracielle et al, 2009). In Formicidae (Lino-Neto and Dolder, 2002;Moya et al, 2007) and other bee species, including Dialictus spp. (Fiorillo et al, 2005), Xylocopini and the solitary bees Thygater (Thygater) analis and Melitoma segmentaria (Araujo, 2009b), the chromatin is compact but has several electron-lucid gaps.…”

Section: Discussionmentioning

confidence: 98%

Morphology of spermatozoa of Dissomphalus connubialis (Ashmead, 1893) (Hymenoptera: Bethylidae)

Oliveira

Fraga

Brito

et al. 2013

Micron

View full text Add to dashboard Cite

In this study, the morphology of spermatozoa of Bethylidae Dissomphalus connubialis (Pristocerinae) was analyzed using light and transmission electron microscopy. Spermatozoa of this species are thin, measure approximately 130 μm in length and comprise a head region and flagellum region. The head is formed by the acrosome and nucleus. The acrosome consists of the acrosome vesicle and the perforatorium, the posterior portion of which is inserted into a cavity at the anterior extremity of the nucleus. The nucleus is compact, electron-dense and measures 15 μm in length. The flagellum possesses two mitochondrial derivatives, two accessory bodies and one axoneme with a 9+9+2 microtubular pattern. The nucleus is connected to the flagellum by the centriole adjunct. Mitochondrial derivatives are compact, apparently without paracrystalline material and with rare mitochondrial cristae. They are asymmetric in length, such that the larger mitochondrial derivative begins parallel to the posterior region of the nucleus and the smaller mitochondrial derivative begins just below the centriole adjunct. The basic structure of spermatozoa of D. connubialis is similar to that of other Aculeata studied. However, this species shows characteristics not seen in other Hymenoptera, such as the wide electron-lucid region that separates the acrosomal vesicle from the perforatorium and the depth of the cavity in the anterior extremity of the nucleus, into which the base of the perforatorium is inserted. There are also characteristics that distinguish this species from Bethylidae Prorops nasuta, including the fact that one of the mitochondrial derivatives lies to parallel to the nucleus over a long distance, the small quantity of cristae, the absence of paracrystalline material in these organelles, and the fact that the accessory microtubules are the first to terminate in the final portion of the flagellum.

show abstract

“…Spermatodesmata with 2 4 to 2 9 sperm cells are regularly found in the seminal vesicles of sawflies (suborder Symphyta) [13]–[15] and bundles or fragments of bundles have been observed also in a few apid bees [16]–[18]. In contrast, studies on spermatogenesis and sperm morphology in other Hymenoptera, including ants [see Table S1], show that sperm bundles generally disintegrate when the males mature and the seminal vesicles only contain free sperm cells [19]–[23].…”

Section: Introductionmentioning

confidence: 99%

Sperm Bundles in the Seminal Vesicles of Sexually Mature Lasius Ant Males

Burnett¹,

Heınze

2014

PLoS ONE

View full text Add to dashboard Cite

In many insects, sperm cells are produced in bundles with their heads being held together by a glycoprotein matrix secreted by a cyst cell. Mature sperm cells in the seminal vesicles are usually free, but in sawflies and several other insects, such structures (spermatodesmata) remain intact and sperm cells may be ejaculated as bundles. Here we report the occurrence of spermatodesmata in mature males of the ant Lasius pallitarsis. Microscopic investigations of the abdominal contents of males immediately prior to their nuptial flights showed that the anterior ends of numerous sperm cells were embedded in an oval-shaped 20 by 30 micrometer extracellular fibrous cap. Individual sperm ranged in length from 55 to 75 micrometers with an average overall length of 65 micrometers. The bulb-shaped heads of the sperm were relatively small, only about 1.5 micrometers in length and about 1.1 micrometers in diameter. The diameter of the sperm tails was approximately 1 micrometer. Observations of live preparations of the spermatodesmata showed increasingly active undulating wave-like movement of the sperm tails as the slide preparations aged. This appears to be the first case of sperm bundles being present in the seminal vesicles of mature ant males – males that are immediately poised to complete their nuptial mating flight.

show abstract

Sperm ultrastructure of five species of the Neotropical ant genus Pseudomyrmex (Hymenoptera: Formicidae)

Cited by 18 publications

References 27 publications

Structural and ultrastructural characteristics of male reproductive tract and spermatozoa in two Cryptinae species (Hymenoptera: Ichneumonidae)

Structural and ultrastructural characteristics of male reproductive tract and spermatozoa in two Cryptinae species (Hymenoptera: Ichneumonidae)

Morphology of spermatozoa of Dissomphalus connubialis (Ashmead, 1893) (Hymenoptera: Bethylidae)

Sperm Bundles in the Seminal Vesicles of Sexually Mature Lasius Ant Males

Contact Info

Product

Resources

About