Sperm Viability: A Comparison of Analytical Methods

Schrader, Steven M.; Platek, S. Frank; Zaneveld, L. J. D.; Perez‐Pelaez, M.; Jeyendran, Rajasingam S.

doi:10.1111/j.1439-0272.1986.tb01822.x

Cited by 46 publications

(11 citation statements)

References 8 publications

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“…by exposing the cell to the hypo-osmotic conditions when water gets into the interior of the sperm cell leading to the increase in its volume resulting in the plasma membrane swelling and twisting of the tail of the sperm [17,18]. To make the fertilization occur, it is necessary to have the sperm membrane integrity preserved both physically and biochemically, and this process can be checked by the HOS test, i.e.…”

Section: Discussionmentioning

confidence: 99%

Activity of superoxide dismutase and catalase and content of malondialdehyde in seminal plasma of infertile patients

Zelen¹,

Mitrović²,

Jurisic-Skevin

et al. 2010

Med pregl

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The reactive oxygen species, the highly reactive metabolites of oxygen, play a crucial role in both the normal function and the metabolism of sperm cells. Oxygen radicals achieve their physiological effects in the cells only if there is a proper balance between their production and degradation. In case of radicals' production exceeding the antioxidant capacity of the semen, there is an oxidative damage of the membrane lipids and proteins as well as the DNA damage followed by the fragmentation and decondensation of DNA. The ejaculates were obtained from seventy-seven infertile and fertile healthy individuals. The semen samples were collected and classified according to the WHO criteria. The activities of superoxide dismutase and catalase as well as the concentration of malondialdehyde were measured spectrophotometrically. The fertile, healthy donors showed the significantly higher activities of both superoxide dismutase and catalase, as well as the lower concentration of malondialdehyde compared to the infertile donors. The activities of superoxide dismutase and catalase, as well as the HOS test, correlated positively with the sperm cell number, but negatively with the concentration of malondialdehyde. The activity of superoxide dismutase and the concentration of malondialdehyde were highest in the group of patients with the lowest success of the HOS test. The assessment of the antioxidant enzymes and malondialdehyde in addition to the semen analysis and the HOS test may be greatly useful in diagnosing infertility in men having oxidative stress in their etiology.

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Section: Discussionmentioning

confidence: 99%

Activity of superoxide dismutase and catalase and content of malondialdehyde in seminal plasma of infertile patients

Zelen¹,

Mitrović²,

Jurisic-Skevin

et al. 2010

Med pregl

View full text Add to dashboard Cite

show abstract

“…Sperm quality evaluation by eosin and nigrosin staining was based on the dye exclusion principle. Eosin and nigrosin cannot penetrate the plasma membrane of live cells but can enter dead cells and stain them; thus assessing the structural integrity of the sperm plasma membrane (Schrader et al 1986). Further studies are necessary to clarify the effect of storage period on sperm viability of preserved spermatophores using mineral oil, and to explore other extenders to prolong sperm viability of black tiger shrimp spermatophores during chilled storage.…”

Section: Discussionmentioning

confidence: 99%

Preservation of Black Tiger Shrimp Penaeus monodon Spermatophores by Chilled Storage

Nimrat

Sangnawakij

Vuthiphandchai

2007

Journal of the World Aquaculture Society

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Chilled storage of spermatozoa in fish has been extensively investigated for many years, but limited research was focused on crustacean species. Chilled storage of spermatophores of black tiger shrimp Penaeus monodon is needed to generate consistent and reliable supply of spermatozoa for subsequent use. The objective of this study was to develop a protocol for the chilled storage of black tiger shrimp spermatophores and to evaluate bacterial propagation during chilled storage of spermatophores. In the first experiment, spermatophores were selected and preserved using four different extenders, namely mineral oil, Ringer's solution, phosphate buffer and 0.85% sodium chloride, and stored at low temperature (2-4 C) for 42 d without antibiotic supplementation. Results showed that mineral oil was the best extender for chilled storage of spermatophores, since the highest percentage of viable sperm (58.3 f 2.9%) was observed with this extender at the end of experiment (day 42). Bacillus sp., Staphylococcus sp., and Pseudomom aeruginosa were identified as the predominant bacteria occurring during chilled storage, and the total bacteria count gradually increased during the experiment. In the second experiment, spermatophores were preserved in the mineral oil with four concentrations of the antibiotic, penicillin-streptomycin (O.l%, 1%,2%, and 3%). There was no significant difference (P> 0.05) in the percentage of viable sperm among treatments with 0.1%, 1 %, 2%, and 3% antibiotics. The total count of Bacillus sp., Staphylococcus sp., and f? aeruginosa in the antibiotic treated groups significantly decreased (P < 0.05) to undetectable levels by day 14 of the experiment. Fertility studies from artificial insemination indicated that l? monodon spermatophores preserved with mineral oil for 7-8 d at 2-4 C were capable of fertilizing eggs with hatching rates similar to the controls. This study suggests that chilled storage of spermatophores is a feasible approach for the management and spawning of black tiger prawn broodstock or other invertebrate species that produce spermatophores.

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“…En otro estudio, donde se evaluó la integridad acrosómica de espermatozoides caninos incubados en agua bidestilada por 5 min a 37 ºC, No obstante haberse descrito una correlación positiva en los porcentajes de dilatación espermática entre HOST-s y HOST (Sánchez y Garrido, 2013), en el presente estudio se pudo observar una drástica reducción en la VE posterior a la incubación con la solución 0 mOsm/L respecto a la incubación con solución 150 mOsm/l (p<0.01). Esta situación permite postular que soluciones de osmolaridad muy baja, causarían además de la respuesta fisiológica de equilibrio osmótico inicial, una mayor cantidad de daño físico en los espermatozoides en el tiempo; esto considerando que la tinción supravital mide la ruptura de la membrana plasmática en el espermatozoide muerto -integridad física-, mientras que HOST evalúa la integridad funcional de la misma -integridad bioquímica- (Schrader et al, 1986).…”

Section: Resultados Y Discusiónunclassified

Efecto del Medio Hipoosmótico sobre la Vitalidad Espermática en Semen Canino

Sánchez¹,

Zamora²

2016

Rev. investig. vet. Perú

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RESUMENLa prueba hipoosmótica ha sido utilizada ampliamente en la valoración de la calidad seminal en varias especies animales. El presente estudio tuvo la finalidad de comparar el efecto de la incubación de espermatozoides caninos en dos soluciones hipoosmóticas (0 mOsm/l -HOST-s y 150 mOsm/l -HOST) sobre la vitalidad espermática (VE). Se obtuvieron 15 eyaculados (2ª fracción) mediante manipulación digital en 10 perros, cuyos espermiogramas fueron considerados normales. De cada eyaculado se tomaron alícuotas de 5 µl para diluirlas en 45 µl de cada solución hipoosmótica, e incubarlas a 37 ºC por 5 y 45 min, respectivamente. La VE se evaluó mediante tinción eosina-nigrosina. En la prueba hipoosmótica se obtuvo 92.1 y 90.1% de espermatozoides dilatados para HOST-s y HOST, respectivamente (p<0.05) y en la prueba de VE se obtuvo 66.5 y 78.3% de espermatozoides vivos para HOST-s y HOST, respectivamente (p<0.01). Se concluye que la menor osmolaridad genera menor VE posincubación. Palabras clave: espermatozoide canino, prueba hipoosmótica, vitalidad espermática ABSTRACTThe hypoosmotic test is widely used for determining sperm quality in several animal species. The present study aimed to compare the effect of incubation of canine sperm into two hypoosmotic solutions (0 mOsm/l -HOST-s and 150 mOsm/l -HOST) on sperm viability. Fifteen ejaculates (2 nd fraction) were obtained by digital manipulation from 10 dogs and the spermiograms were considered as normals. A 5 µl aliquots per ejaculate were diluted in 45 µl of each of the two hypoosmotic solutions and incubated at 37 °C for

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Sperm Viability: A Comparison of Analytical Methods

Cited by 46 publications

References 8 publications

Activity of superoxide dismutase and catalase and content of malondialdehyde in seminal plasma of infertile patients

Activity of superoxide dismutase and catalase and content of malondialdehyde in seminal plasma of infertile patients

Preservation of Black Tiger Shrimp Penaeus monodon Spermatophores by Chilled Storage

Efecto del Medio Hipoosmótico sobre la Vitalidad Espermática en Semen Canino

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