Sperm volumetric dynamics during in vitro capacitation process in bovine spermatozoa

García-Herreros, Manuel; Leal, Cláudia Lima Verde

doi:10.1017/s1751731115000129

Cited by 9 publications

(5 citation statements)

References 40 publications

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“…Many studies indicate a relationship between sperm dimensions and fertility in horses (Hidalgo, Rodríguez, Dorado, & Soler, ; Phetudomsinsuk, Sirinarumitr, Laikul, & Pinyopummin, ), in which fertility disorders have been positively correlated with increased sperm head size (Gravance, Liu, Davis, Hughes, & Casey, ). The subject has also been taken up with regard to fertility in pigs (Saravia et al, ), bulls (Garcia‐Herreros & Leal, ) and dogs (Núñez‐Martínez, Moran, & Peña, ). Differences in head size between fertile and infertile males have been detected in various species (Casey, Gravance, Davis, Chabot, & Liu, ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the structure of chinchilla sperm isolated from semen and from the tail of the epididymis

Andraszek

Banaszewska

Szeleszczuk

et al. 2020

Reprod Domestic Animals

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Sperm cells isolated from the tail of the epididymis and from the semen of the same individuals were analysed. The use of silver nitrate to stain sperm cells isolated from the tail of the epididymis made it possible to identify structures that were not visible in the sperm from semen. Silver nitrate very clearly distinguished the acrosomal and distal parts of the sperm head. Following silver nitrate staining, the sperm isolated from the tail of the epididymis were characterized by dark ‘collars’ in the distal part of the head. These ‘collars’ are not visible in the sperm cells isolated from semen. The results of the study indicate differences in the dimensions of sperm isolated from the tail of the epididymis and sperm in semen. Sperm isolated from the tail of the epididymis had smaller heads, despite their longer length, and had longer midpieces and tails than ejaculate sperm. Silver nitrate staining is a simple and fast technique. Silver nitrate makes it possible to identify the acrosome and post‐acrosomal region of the sperm head and to clearly identify the midpiece. Therefore, it can be successfully used to supplement routine techniques for evaluating sperm morphology or as an independent technique.

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Section: Discussionmentioning

confidence: 99%

Comparison of the structure of chinchilla sperm isolated from semen and from the tail of the epididymis

Andraszek

Banaszewska

Szeleszczuk

et al. 2020

Reprod Domestic Animals

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“…Although the in vivo condition is considered to be more efficient as one capacitated sperm is enough for the completion of fertilization for each oocyte (Muro et al, 2016), in vitro capacitation medium for mammalian sperm has also been continuously refined through the introduction of several capacitation inducers and modification of procedure with different approach, as compiled in Table 3. (García-Álvarez et al, 2015;García-Herreros and Leal, 2014;Lukoseviciute et al, 2004;Parrish et al, 1988). There is also an observable trend where the consideration of the use of capacitation inducers leads to recreating the physiological environment of the female genital tract (Chaves et al, 2021), using substances that have been identified to modulate sperm capacitation in vivo such as oviductal fluid (OF) (Navarro-Serna et al, 2021), creatine (Umehara et al, 2018), and the involvement of hormones (estrogen and progesterone) (García-Álvarez et al, 2015).…”

Section: Flaws and Improvementsmentioning

confidence: 99%

Mammalian sperm capacitation: In vivo and in vitro juxtaposition

Setiawan¹,

Chuammitri²,

Sringarm³

et al. 2022

VIS

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The development of assisted reproductive technologies (ART) in mammalian species such as in vitro embryo production (IVEP) has the potential to provide great benefits for significant population increase, improve genetic performance and advancement, and reduce transmission of venereal diseases. Correspondingly, in vitro capacitation of sperm is also paramount, related to the ability of sperm to fertilize oocytes, and was created to imitate in vivo conditions in the female reproductive tract. Amid in vitro capacitation developments, studies on how far in vitro capacitation has progressed in mimicking in vivo scenes have not been thoroughly reviewed as a comparative form. Therefore, the present study outlined the series of alterations in mammalian sperm capacitation during their journey in the female reproductive tract by exploring and juxtaposing processes under in vivo and in vitro conditions. Several essential aspects that become gaps between in vivo and in vitro were also identified and elaborated comprehensively in this systematic literature review. We noted that although in vitro capacitation procedures in certain mammalian species have made promising progress and improvements, it is still poorly successful in other species like horses. Our findings further postulated that the occurrence of cryocapacitation, the high ratio of capacitated sperm/oocyte required for successful fertilization, and the incidence of polyspermy cause capacitation under in vitro settings is less efficient and not yet fully comparable to in vivo. This work is therefore proposed several aspects that need to be bettered from in vitro milieu to make it analogous to in vivo environments in modulating sperm capacitation.

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“…Some authors have explored the morphological response to diluting or washing the sperm sample,105106 to capacitation107 or to cryopreservation 26108109110…”

Section: Assessment Of Sperm Morphometry II – Functional Aspectsmentioning

confidence: 99%

“…reported how boar spermatozoa in the female reproductive tract are selected on the basis of their size and shape, with those with a larger head and longer tail being those reaching the fertilization site. For sperm morphometric changes during capacitation, García-Herreros and Leal107 reported that the induction of in vitro capacitation in bovine spermatozoa modified sperm head morphometry. As capacitated spermatozoa showed a decrease in all sperm head size and shape parameters, the authors concluded that sperm head morphometry is an objective diagnostic tool for sperm assessment during capacitation.…”

Section: Assessment Of Sperm Morphometry II – Functional Aspectsmentioning

confidence: 99%

Current status and potential of morphometric sperm analysis

et al. 2016

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The spermatozoon is the most diverse cell type known and this diversity is considered to reflect differences in sperm function. How the diversity in sperm morphology arose during speciation and what role the different specializations play in sperm function, however, remain incompletely characterized. This work reviews the hypotheses proposed to explain sperm morphological evolution, with a focus on some aspects of sperm morphometric evaluation; the ability of morphometrics to predict sperm cryoresistance and male fertility is also discussed. For this, the evaluation of patterns of change of sperm head morphometry throughout a process, instead of the study of the morphometric characteristics of the sperm head at different stages, allows a better identification of the males with different sperm cryoconservation ability. These new approaches, together with more studies employing a greater number of individuals, are needed to obtain novel results concerning the role of sperm morphometry on sperm function. Future studies should aim at understanding the causes of sperm design diversity and the mechanisms that generate them, giving increased attention to other sperm structures besides the sperm head. The implementation of scientific and technological advances could benefit the simultaneous examination of sperm phenotype and sperm function, demonstrating that sperm morphometry could be a useful tool for sperm assessment.

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Sperm volumetric dynamics during in vitro capacitation process in bovine spermatozoa

Cited by 9 publications

References 40 publications

Comparison of the structure of chinchilla sperm isolated from semen and from the tail of the epididymis

Comparison of the structure of chinchilla sperm isolated from semen and from the tail of the epididymis

Mammalian sperm capacitation: In vivo and in vitro juxtaposition

Current status and potential of morphometric sperm analysis

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