Spermatogenesis after Transplantation of Adipose Tissue-Derived Stem Cells in Azoospermic Guinea Pigs: A Histological and Histomorphometric Study

Abstract

Background: The purpose of this research was to determine histomorphometric changes in busulfan-induced azoospermia after transplantation of AdSCs in guinea pig. AdSCs were isolated from adipose tissue around the testes of guinea pigs and characterized for mesenchymal properties.Materials and Methods: Guinea pigs were allocated into three groups, including the control group without any intervention. To induce azoospermia, groups 2 and 3 received a dose of 40 mg/kg of busulfan with 21 days interval. Group 3 received 1×106 AdSCs in their seminiferous tubules of left testes, 35 days following last busulfan injection, while right testis in the group was considered for comparison as controls. Sixty days following transplantation of cell, histomorphometric and histopathologic changes of the experiments were assessed.Results: After AdSCs’ transplantation, normal spermatogenesis appearance was noticed compared to busulfan-induced azoospermia and AdSCs recovered spermatogenesis, and our findings can be added to the literature in treating azoospermic infertilities.Conclusion: The transplanted AdSCs could induce production of germinal cells using testicular seminiferous tubules and were an effective source in treating azoospermia.[GMJ.2018;7:e1000]

Glucocorticoid exposure modifies the miRNA profile of sperm in the guinea pig: Implications for intergenerational transmission

“…Guinea pigs were exposed to the sGC dexamethasone 21‐phospate disodium salt in the drinking water (Dex; 3 mg/kg; Sigma Aldrich, St. Louis, MO) every other day for 48 days ( n = 6). This duration of exposure encompasses the entire spermatogenic process of the guinea pig 30 so that sperm were exposed to sGC at multiple stages of spermatogenesis as well as maturation during epididymal transit. Controls received untreated water ( n = 6).…”

Effects of clinical medications on male fertility and prospects for stem cell therapy

“…ADSCs and AFSCs can reduce oxidative stress in the testes and promote sperm production, ( Eliyasi et al, 2020 ; Ibrahim et al, 2021 ; Qian et al, 2020 ; Siregar et al, 2021 ), and can improve the damage of chemotherapy drugs to testes ( Meligy et al, 2019 ). Intratesticular injection of ADSCs can improve the spermatogenesis of azoospermia ( Ganjibakhsh et al, 2022 ; Hajihoseini et al, 2018 ; Karimaghai et al, 2018 ), while xenotransplantation of human umbilical cord blood derived MSCs into azoospermia mice also has the same effect ( Abd et al, 2017 ). Animal experiments have shown that after transplantation of BM-MSCs into the testis, in addition to influencing male reproductive function through paracrine action, they will also relocate to a new niche to form colonies, thus realizing the reconstruction of the damaged testicular germinal epithelium ( Ghasemzadeh-Hasankolaei et al, 2016 ; Monsefi et al, 2013 ).…”