Spermatogenesis and cellular associations in the seminiferous epithelium of Guinea cock (<i>Numida meleagris</i>)

Abdul-Rahman, Ibn Iddriss; Obese, F.Y.; Robinson, Jane E.

doi:10.1139/cjas-2016-0068

Cited by 6 publications

(6 citation statements)

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“…One of the most important findings was the presence of leptotene primary spermatocytes in all stages of spermiogenesis and their long life span. These results are in accordance with previous reports in the duck [36], guinea fowl [35], Japanese quail [16] and greater Rhea [38] where leptotene, pachytene and zygotene primary spermatocytes were observed in the seminiferous epithelium of the relevant birds. Secondary spermatocytes are rarely observed in the seminiferous epithelium because their life span is very short [39].…”

Section: Discussionsupporting

confidence: 93%

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Spermiogenesis, Stages of Seminiferous Epithelium and Variations in Seminiferous Tubules during Active States of Spermatogenesis in Yangzhou Goose Ganders

Akhtar

Ahmad

Mustafa

et al. 2020

Animals

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The past three decades revolutionized the goose industry in the world. China holds the world’s largest goose breeds stock by 95% of the global total. To optimize the goose industry and cope with ever increasing poultry meat and egg demands, there is a dire need to focus on reproduction, as most geese breeds exhibit poor reproductive performance. The present study was conducted with the aim to add a contribution in the goose industry and research by the histological visualizing step wise development of germ cells during spermatogenesis by microscopy and a histological technique. Yangzhou goose is a synthetic breed developed by using local goose germplasm resources of China. It is popular in the Chinese goose industry due to high productivity and adaptability. This research evaluated the steps of spermiogenesis and stages along with morphological changes in the seminiferous epithelium in Yangzhou goose ganders. For the assessment of various stages of the seminiferous epithelium cycle, testis sections were embedded in molten paraffin wax. The initial steps of spermiogenesis were depicted by changes in acrosomic granules, whereas further stages were identified by nuclear morphological changes. Ten steps of spermiogenesis and nine stages of seminiferous epithelium were identified. Four types of spermatogonia Ad, Ap1, Ap2 and B were recognized. The results depicted a clear variation in the diameter of seminiferous tubules (ST), epithelium height (EH), luminal tubular diameter (LD), number of seminiferous tubules per field and the Johnsen score. Microscopy indicated that the stages of seminiferous epithelium were similar to other birds and mammals and the ST diameter, EH, LD and Johnsen score are positively correlated while the number of seminiferous tubules per field is negatively correlated with the ST diameter, EH, LD and Johnsen score. Fertility in Yangzhou ganders can further be improved by visualizing the histological development of germs cells in testis tissues during spermatogenesis after onset of breeding season and maturity. Our results suggest that Yangzhou ganders reach complete sexual maturity at 227 days of age.

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Section: Discussionsupporting

confidence: 93%

“…The cap phase that characterizes mammalian spermiogenesis [34] during spermatids development was absent in all stages of the cycle in Yangzhou ganders. These reports are in accordance with the guinea fowl [35], drakes [36] and in the Japanese quail [37].…”

Section: Discussionsupporting

confidence: 87%

Spermiogenesis, Stages of Seminiferous Epithelium and Variations in Seminiferous Tubules during Active States of Spermatogenesis in Yangzhou Goose Ganders

Akhtar

Ahmad

Mustafa

et al. 2020

Animals

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“…Different stages of spermatid and seven cellular associations could be identified in doves from the present study. Conversely, nine cellular associations have been observed on testes of guinea cock (Abdul-Rahman et al, 2017) and eight cellular associations in guinea fowl (Aire et al, 1980).…”

Section: Discussionmentioning

confidence: 97%

Histological, histochemical, immunohistochemical and ultrastructural characterization of the testes of the dove

Mustafa

Elhanbaly

2020

Zygote

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Summary Avian testes have been used in the study of germ cell transfer, importantly for understanding the preservation and control of birds. For this purpose, we use light microscopy, electron microscopy and immunohistochemistry to understand the reproductive efficiency of dove testes. The tunica albuginea was thin and septula testes were not observed. The testicular parenchyma was formed mainly of closely packed convoluted seminiferous tubules with little interstitial area. Three types of spermatogonia were distinguished. The primary spermatocyte appeared as the largest spermatogenic cell and was identified at different stages of meiosis. Different morphological stages of the spermatid were categorized. Various cellular associations were described within the seminiferous epithelium. The cytoplasm of Sertoli cells was pale and ill defined due to its close relationship to the germinal epithelium. The spermatid attached to the luminal border of Sertoli cells and germ cells were closely associated. A single layer of myoid cells surrounded the seminiferous tubule. Testicular telocytes of doves were located in the peritubular region and near the blood vessels. Telopods appeared as long cytoplasmic processes arising from the cell body. Leydig cells were distributed singly or in small groups and cords. The intensity of androgen receptor (AR) immunostaining in the testes of the dove was established for the first time and is described in this paper.

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“…The utilization of this staining for acrosome formation observation in each stage of SE during spermatogenesis was successfully applied to the testicular tissue of long-tailed macaques [ 19 ] and rams [ 20 ]. Acrosome granules that are detected in round spermatids by PAS staining can be used to identify the initial steps of spermatid development, namely, spermiogenesis [ 41 ]. The most important morphologic changes during spermiogenesis are formation of the acrosome, condensation of the nuclear chromatin, outgrowth of motile tail of sperm, and loss of excess spermatid material such as cytoplasm, water, and also organelles that are not necessary for the later spermatozoa [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Identification and Determination of the Seminiferous Epithelium Stages and Spermatid Development in the Testis of Aceh Bull (Bos indicus)

Wahyuni,

Siregar,

Gholib

et al. 2023

Veterinary Medicine International

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This study was conducted to describe the stages of seminiferous epithelium (SE), determine the relative frequency of the stages, and identify the steps of spermatid development during spermatogenesis in the testicular tissue of Aceh bull. Seven pairs of the testicular organs of Aceh bull (Bos indicus) were used and then processed in a histological manner for staining using haematoxylin and eosin (H&E) and periodic acid-Schiff-haematoxylin (PAS-H). The stages of seminiferous tubules were examined using a tubular morphology method while spermatid development was observed based on the acrosome formation during spermatid development. Eight stages (stages I to VIII) of SE were found in the testicular seminiferous tubules of Aceh bull. Furthermore, the percentage of the relative frequency of each stage was 25.48, 15.38, 12.92, 4.74, 14.97, 10.69, 10.74, and 5.08%, respectively, with the relative frequency of premeiotic, meiotic, and postmeiotic phases being 53.78, 4.74, and 41.48%, respectively. Spermatid development from round to elongated spermatids occurred in 14 steps. Steps 1 to 7 were observed in stage I, steps 8 and 9 in stage II, steps 10 and 11 in stage III, step 12 in stage IV, step 13 in stages V and VI, and step 14 in stages VII and VIII. These findings can be used as a basis for further studies, particularly in evaluating the abnormality of the cellular composition of the seminiferous tubule in each stage of spermatogenesis and also in determining daily sperm production in Aceh bull.

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Spermatogenesis and cellular associations in the seminiferous epithelium of Guinea cock (Numida meleagris)

Abstract: Light and the Light Perception of BirdsLight is the visible part of the electromagnetic radiation. It plays an important role for the life of many organisms on earth. Light is perceived by them in different ways.

Cited by 6 publications

References 20 publications

Spermiogenesis, Stages of Seminiferous Epithelium and Variations in Seminiferous Tubules during Active States of Spermatogenesis in Yangzhou Goose Ganders

Spermiogenesis, Stages of Seminiferous Epithelium and Variations in Seminiferous Tubules during Active States of Spermatogenesis in Yangzhou Goose Ganders

Histological, histochemical, immunohistochemical and ultrastructural characterization of the testes of the dove

Identification and Determination of the Seminiferous Epithelium Stages and Spermatid Development in the Testis of Aceh Bull (Bos indicus)

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