Spermatogenesis and sexual maturation in male<i>Mactra chinensis</i>(Bivalvia: Mactridae) of Korea

Chung, Ee‐Yung; Kim, Eun-Jong; Park, Gab Man

doi:10.1080/17386357.2007.9647339

Cited by 18 publications

(27 citation statements)

References 9 publications

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“…martensii shows some similar phenomena to those of other bivalves (Eckelbarger et al, 1990;Chung et al, 2007;Kim et al, 2010b). Recently, Sousa et al, (1989) suggested that the Golgi complex may form only a single acrosomal vesicle in a manner similar to other molluscs.…”

Section: Discussionsupporting

confidence: 55%

“…Utrastructural studies have been described the spermatogenesis and spermatozoa of a number of bivalve species using electron microscopy (Longo and Dornfelds, 1967;Longo and Anderson, 1969;Popham, 1979;Bernard and Hodgson, 1985;Hodgson and Bernard, 1986;Healy, 1989;Eckelbarger et al, 1990;Eckelbarger and Davis, 1996;Gaulejac et al, 1995;Chung and Ryou, 2000;Chung et al, 2007Chung et al, , 2010.…”

Section: Introductionmentioning

confidence: 99%

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Germ Cell Differentiations During Spermatogenensis and Taxonomic Values of Mature Sperm Morphology of Pinctada martensii (Bivalvia, Pteriomorphia, Pteriidae)

Kim¹,

Kim²,

Lee³

2011

The Korean Journal of Malacology

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The ultrastructural characteristics of germ cells during spermatogenesis and mature sperm morphology in male Pinctada martensii were investigated by transmission electron microscope observation. The morphologies of the sperm nucleus and the acrosome of this species are the oval shape and cone shape, respectively. Spermatozoa are approximately 47-50 μm in length including a sperm nucleus (about 1.24 μm in length), an acrosome (about 0.60 μm in length), and tail flagellum (about 45-47μm). The axoneme of the sperm tail shows a 9+2 structure. In P. martensii in Pteriidae, a special substructure showing a thick and wide triangular shape which is composed of electron-dense opaque material (occupied about 50% of all, the upper part of the acrosomal vesicle), appeared in the upper region (part) of the acrosomal vesicle, while the lower region (part) of the acrosomal vesicle is composed of electron-lucent material. Thus, this special structure, which exist in the upper part of the acrosomal vesicle in P. martensii, is somewhat different from those of other subacrosomal vesicle in other families in subacrosomal vesicles. Therefore, we assume that the existence of a special substructure showing a thick and wide triangular shape in the acrosomal vesicle of the spermatozoon can be used as a key characteristic for identification of P. martensii or other species in Pteriidae in subclass Pteriomorphia. The number of mitochondria in the midpiece of the sperm of this species are five (exceptionally sometimes four), as one of common characteristics appear the same number of mitochondria in the same families of superfamilyies. This species in Pteriidae does not contain the axial rod and satellite fibres which appear in the species in Ostreidae in subclass Pteriomorphia. These characteristics can be used for the taxonomic analysis of the family or superfamily levels as a systematic key or tools.

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Section: Discussionsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Germ Cell Differentiations During Spermatogenensis and Taxonomic Values of Mature Sperm Morphology of Pinctada martensii (Bivalvia, Pteriomorphia, Pteriidae)

Kim¹,

Kim²,

Lee³

2011

The Korean Journal of Malacology

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“…irradians irradians showed similar phenomena to those of other bivalves and more specifically of Pteriormorphia species (Eckelbarger et al, 1990;Eckelbarger and Davis, 1996;Gaulejac et al, 1995;Chung et al, 2007Chung et al, , 2010Kim et al, 2010a,b).…”

Section: Spermatogenesismentioning

confidence: 76%

“…Recently, the number of mitochondria in the sperm midpiece have been now widely used in taxonomic analyses because the number of mitochondria in the sperm midpiece tends to be stable within any given family or superfamily (Healy, 1989(Healy, , 1995. To date, many authors (Chung and Ryou, 2000;Kim, 2001;Chung et al, 2007;2010) described that the number of mitochondria in the midpiece of the spermatozoon were four in families Ostreidae and Pectinidae in the subclass Pteriomorphia, and also Veneridae, Solenidae and Corbiculidae in the subclass Heterodonta.…”

Section: Spermatogenesismentioning

confidence: 99%

“…To date, many studies have shown that most bivalves have primitive spermatozoa (Franzen, 1983;Chung et al, 2007;Kim et al, 2010a,b), typical bivalves which release their gametes into the surrouding water (Franzen, 1983;Gaulejac et al, 1995;Eckelbarger et al, 1990;Kim, 2001;Chung et al, 2007;Kim et al, 2010a,b). In this study, during the process of spermatogenesis of germ cells, the synaptonemal complexes in the nucleus of the primary spermatocyte appeared in the pachytene stage in the prophase during the first maturation division.…”

Section: Spermatogenesismentioning

confidence: 99%

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Germ Cell Development During Spermatogenesis and Taxonomic Values in Mature sperm Morphology in Male Argopecten irradians irradians (Pteriomorphia: Pectinidae) in Southern Korea

Kim¹,

Chung²,

Park³

2012

The Korean Journal of Malacology

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Ultrastructural studies of germ cell development during spermatogenesis and taxonomic values in mature sperm morphology of Argopecten irradians irradians were investigated by transmission electron microscopic observations. In the early stage of spermatid during spermiogenesis, a few granules and proacrosomal granules are formed by the Golgi complex. In the late stage of spermatid during spermiogenesis, a proacrosomal vesicle becomes an acrosomal vesicle in the acrosome through spermiogenesis. The sperm is approximately 45-48 μm in length including a jar-shaped sperm nucleus (about 1.45 μm long), an acrosome (about 0.34 μm long) and tail flagellum. The axoneme of the sperm tail shows a 9+2 structure. As one of common characteristics of mature sperm morphologies in Pectinidae species in subclass Pteriomorphia, mature spermatozoon consists of the cone-shaped acrosomal vesicle and subacrosomal material on the invaginated jar-shaped nucleus. The acrosomal vesicle of this species is composed of electron high dense opaque part (material) from the base to the tip, as have seen in the species in the subclass Pteriomorphia. Exceptionally, five mitochondria are found in the sperm midpiece of this species, unlike four in most species of Pectinidae in subclass Pteriomorphia. However, the acrosomal vesicle of spermatozoa of A. irradians irradians resemble to those of other investigated Pectinidae species in subclass Pteriomorphia. Therefore, we can use sperm morphology as a tool in the resolution of taxonomic relationships within the Pectinidae species. These morphological charateristics of acrosomal vesicle belong to the family Pectinidae in the subclass Pteriomorphia.

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Isolation and characterization of 19 microsatellite markers from the Chinese surf clam (Mactra chinensis)

Kong

2009

Conservation Genet Resour

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The Chinese surf clam Mactra chinensis is a commercially important bivalve, which has experienced severe population declines due to over-fishing and deterioration of coastal environment. The first set of 19 polymorphic microsatellite loci was developed and characterized for population studies. The number of alleles and expected heterozygosity ranged from 3 to 24 and from 0.419 to 0.953, respectively. The average polymorphic information content was 0.790. These microsatellite loci will facilitate future studies of population structure and conservation genetics in this species.Keywords Mactra chinensis Á Microsatellite Á FIASCO protocol Á Chinese surf clam The Chinese surf clam (Mactra chinensis), a bivalve distributed along the coastal areas of East Asia, is one of the popular fisheries resources in China. Due to over-fishing and deterioration of the coastal environment, natural populations of M. chinensis have been declining dramatically for the past decades. Demands for conservation and production improvement have resulted in growing research interests of this species (Sakurai et al. 1998;Chung et al. 2007). Assessing the genetic structure of wild populations and monitoring the genetic diversities of cultivated stocks are essential for sustainable management, genetic improvement and to avoid genetic erosion in the species. For their high variability, neutrality and codominant inheritance (Weber and May 1989), microsatellite markers provide an effective tool for investigation of genetic variation and population structure. Therefore, there is an urgent need for the development and application of microsatellites in M. chinensis by both the scientific community and the breeding industry. In this study, we reported the first set of microsatellite markers of M. chinensis, and examined the potential use of these markers for pedigree analysis.The microsatellite markers of M. chinensis were developed using the FIASCO (Fast Isolation by AFLP of Sequences Containing repeats) protocol (Zane et al. 2002) with some modifications. Briefly, genomic DNA was extracted from the adductor muscle of one live M. chinensis captured from Haiyang, Shandong province, China, using a modified phenol-chloroform protocol (Li et al. 2006). About 200 ng genomic DNA was digested with the restriction enzyme MseI, and then the fragments were ligated to MseI AFLP adaptor. The digested-ligated DNA was amplified by polymerase chain reaction (PCR) with the following reaction mixture: 2 ll buffer 19 (TaKaRa Bio Inc., Shiga, Japan), 1.5 mM MgCl 2 , 0.25 lM of primer MseI-N (5 0 -GATGAGTCCTGAGTAAN-3 0 ), 0.2 mM of each dNTP, 0.5 units of Taq DNA polymerase and 5 ll of a 1/10 dilution of digested-ligated DNA. The reaction was incubated in a GeneAmp PCR system 9700 (Applied Biosystems, Foster City, CA, USA) set to 17 cycles of 94°C for 30 s, 53°C for 1 min, and 72°C for 1 min.The amplified products were hybridized to two biotinylated probes [(CA) 15 and (CT) 15 ]. The biotinylated fragments-probe complexes were captured by streptavidincoated beads (Promega...

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Spermatogenesis and sexual maturation in maleMactra chinensis(Bivalvia: Mactridae) of Korea

Cited by 18 publications

References 9 publications

Germ Cell Differentiations During Spermatogenensis and Taxonomic Values of Mature Sperm Morphology of Pinctada martensii (Bivalvia, Pteriomorphia, Pteriidae)

Germ Cell Differentiations During Spermatogenensis and Taxonomic Values of Mature Sperm Morphology of Pinctada martensii (Bivalvia, Pteriomorphia, Pteriidae)

Germ Cell Development During Spermatogenesis and Taxonomic Values in Mature sperm Morphology in Male Argopecten irradians irradians (Pteriomorphia: Pectinidae) in Southern Korea

Isolation and characterization of 19 microsatellite markers from the Chinese surf clam (Mactra chinensis)

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