Spermatogonia-specific proteins expressed in prepubertal buffalo (Bubalus bubalis) testis and their utilization for isolation and in vitro cultivation of spermatogonia

Goel, Sandeep; Reddy, Niranjan; Mandal, Suman; Fujihara, Mayako; Kim, Sung-Min; Imai, Hiroshi

doi:10.1016/j.theriogenology.2010.05.025

Cited by 43 publications

(38 citation statements)

References 45 publications

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“…UCHL1 staining was found in gonocytes and type A single and paired spermatogonia, consistent with previous findings (Luo et al, 2006). Unlike DBA binding, UCHL1 expression is observed in undifferentiated porcine SSCs at all ages, and has been used to identify these cells in pigs, buffalo, cows, and humans (Luo et al, 2006;Goel et al, 2010;He et al, 2010;Fujihara et al, 2011). In the present study, expression of this protein by SSCs in testis tissue was also observed; therefore, it can be used to distinguish Bama minipig SSCs cultured in vitro.…”

Section: Discussionsupporting

confidence: 91%

Isolation, proliferation, and induction of Bama mini-pig spermatogonial stem cells in vitro

Zhao¹,

Yang²,

Luo³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Spermatogonial stem cells (SSCs), the unique seed cells of testes, can undergo meiosis and form spermatozoa, thus transmitting genetic information to offspring. Research concerning these cells explores the mechanism underlying spermatogenesis, making possible the induction of their differentiation into spermatozoa in vitro. SSCs have therefore attracted much interest among scientists. Although the proliferation of such cells in vitro has been demonstrated, we are unaware of any long-term laboratory culture of porcine SSCs. The objective of this study was to isolate, characterize, culture, and induce the differentiation of Bama mini-pig SSCs. SSCs were isolated using differential plating and cultured for over 100 days on an STO feeder cell layer without serum. Cell clusters appeared after three passages and continuously formed during subsequent cultivation. Staining showed that these clusters were positive for UCHL1 and CDH1, could be bound by Dolichos biflorus agglutinin, and that some cells expressed OCT4. Ultrastructure observations revealed SSCs in testis tissue to be round in shape, while those cultured in vitro were flat and bound together. Our attempts at inducing differentiation showed that SSCs cultured in vitro could undergo meiosis. In this study, we describe an effective culture system for Bama mini-pig SSCs capable of producing enough cells to establish a platform for further SSC research, such as genetic manipulation or exploration of the mechanism underlying spermatogenesis.

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Section: Discussionsupporting

confidence: 91%

Isolation, proliferation, and induction of Bama mini-pig spermatogonial stem cells in vitro

Zhao¹,

Yang²,

Luo³

et al. 2016

Genet. Mol. Res.

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“…The testis sections were stained with UCHL1/PGP 9.5 (ubiquitin carboxyterminal hydrolase L1) to mark gonocytes/SG using a procedure described previously (Goel et al 2010). The testis sections were also stained with AMH to assess the SC maturation and PCNA to evaluate cell proliferation in seminiferous tubules of xenografts.…”

Section: Uchl1 Amh and Pcna Immunostaining Of Grafted Testes Sectionsmentioning

confidence: 99%

Gonadal status of male recipient mice influences germ cell development in immature buffalo testis tissue xenograft

Reddy

Mahla²,

Thathi³

et al. 2012

Reproduction

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Growth and development of immature testis xenograft from various domestic mammals has been shown in mouse recipients; however, buffalo testis xenografts have not been reported to date. In this study, small fragments of testis tissue from 8-week-old buffalo calves were implanted subcutaneously onto the back of immunodeficient male mouse recipients, which were either castrated or left intact (non-castrated). The xenografts were retrieved and analyzed 12 and 24 weeks later. The grafted tissue survived and grew in both types of recipient with a significant increase in weight and seminiferous tubule diameter. Recovery of grafts from intact recipients 24 weeks post-grafting was significantly lower than that from the castrated recipients. Seminal vesicle indices and serum testosterone levels were lower in castrated recipients at both collection time points in comparison to the intact recipients and non-grafted intact mouse controls. Pachytene spermatocytes were the most advanced germ cells observed in grafts recovered from castrated recipients 24 weeks post-grafting. Complete spermatogenesis, as indicated by the presence of elongated spermatids, was present only in grafts from intact recipients collected 24 weeks post-grafting. However, significant number of germ cells with DNA damage was also detected in these grafts as indicated by TUNEL assay. The complete germ cell differentiation in xenografts from intact recipients may be attributed to efficient Sertoli cell maturation. These results suggest that germ cell differentiation in buffalo testis xenograft can be completed by altering the recipient gonadal status.

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“…Certain rodent markers for germ cells and SSCs such as VASA, DAZL, PLZF, and GFR1are also of monkey (Hermann et al, 2007) and in other species. VASA expression marks spermatogonia in sheep (Borjigin et al, 2010), buffalo (Goel et al, 2010b) and bull (Fujihara et al, 2011) testis. It has been demonstrated that GFR1 is a marker for mouse SSCs and probably their progeny (Meng et al, 2000;Buageaw et al, 2005;Hofmann et al, 2005;Naughton et al, 2006;He et al, 2007), and that KIT is a characteristic for the more differentiated spermatogonia, including type A 1-4 spermatogonia (Yoshinaga et al, 1991).…”

Section: Markers Of Sscsmentioning

confidence: 99%

“…The GFR1 and POU5F1 antibodies stain the same subset of mouse spermatogonia (He et al, 2007). POU5F1 also marks spermatogonia in buffalo (Goel et al, 2010b) and bull (Fujihara et al, 2011). GPR125 is also believed to be a marker for mouse spermatogonial stem/progenitor cells (Seandel et al, 2007).…”

Section: Markers Of Sscsmentioning

confidence: 99%

“…UCHL-1 is known to express in spermatogonia of mice (Kwon et al, 2004), monkey (Tokunaga et al, 1999) and humans (He et al, 2010). UCHL-1 protein expression is present specifically in the spermatogonia of domestic animal testes such as bull (Wrobel et al, 1995;Herrid et al, 2009), pig (Luo et al, 2006;Goel et al, 2007), sheep (Rodriguez-Sosa et al, 2006) and buffalo (Goel et al, 2010b). UCHL-1 protein expression is also specific to spermatogonia of wild bovids (Goel et al, 2010a).…”

Section: Markers Of Sscsmentioning

confidence: 99%

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Pluripotent Stem Cells from Testis

Goel¹,

Imai²

2011

Embryonic Stem Cells - Differentiation and Pluripotent Alternatives

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Spermatogonia-specific proteins expressed in prepubertal buffalo (Bubalus bubalis) testis and their utilization for isolation and in vitro cultivation of spermatogonia

Cited by 43 publications

References 45 publications

Isolation, proliferation, and induction of Bama mini-pig spermatogonial stem cells in vitro

Isolation, proliferation, and induction of Bama mini-pig spermatogonial stem cells in vitro

Gonadal status of male recipient mice influences germ cell development in immature buffalo testis tissue xenograft

Pluripotent Stem Cells from Testis

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