Spermatozoa Survival Rate in Stored Cock Semen Extended With Diluent Fortified With Natural Antioxidant Sources under Room Temperature

Abstract

An experiment was conducted in a completely randomized design with five treatments in triplicates. Four extenders were formulated: Lake Extender; Coconut water-Orange juice Extender (CWOE); Egg yolk-Tomato juice Extender (EYTE) and Milk based-Carrot juice Extender (MCE), were used to extend cock semen constituting treatments 2, 3, 4 and 5 respectively, while the control (Treatment 1) was unextended semen. Semen was harvested from 8 proven Isa breeder cocks aged 35-40 weeks, pooled and evaluated. The ejaculate was divided into 5 and each part was extended with the extenders at ratio 1:2 (Semen: Extender). The extended and un-extended semen samples were kept under room temperatures (26.6-27.4oC) with relative humidity of 64.0-74.0% for in vitro assessment at 2 hrs interval until percentage motility was less than 60%. The results showed that the mass activity, sperm motility and percentage livability reduced p<0.05) as the time of storage increased. Mass activities of unextended semen and the one extended with coconut water-orange juice were significantly (P<0.05) higher than that of other treatments at 0 hour period of storage. Sperm motility in treatments 1 (95.0%), 3 (93.3%) and 4 (88.3%) was not significantly (p>0.05) different from one another, but they was significantly (p<0.05) higher than treatments 2 (40.0%) and 5 (71.7%). At 2 hours of storage, the mass activity and percentage sperm motility in treatment 2 were significantly (P<0.05) lower than other treatments, while live sperm cells were significantly lower for Treatment 3 than other treatments. At 4 hours of storage, mass activity and motility were zero in Treatment 2, while Treatment 1 recorded the highest values (66.7% and 93.5% respectively). Percent motility in Treatments 3, 4 and 5 were 55.0, 50.0 and 43.3%, respectively. However, percent live sperm cells were significantly (P<0.05) higher in treatments 2, 4, 5 and 1 than In treatment 3. Both CWOE and EYTE possessed a good keeping quality for semen storage. The extenders fortified with natural antioxidants can be used for on-farm insemination in poultry within two hours of collection.

Weiße Eier, braune Eier – blaues Licht, rotes Licht

“…Das rotorange Leuchten, das bei den Schalen brauner Eier zu be obachten ist, kommt von in der Eier Ob Hühner braune, weiße oder grüne Eier legen, folgt nicht etwa dem Zufall, sondern ist genetisch für die jeweilige Legerasse determiniert [5]. Der populärwissenschaftlich kolportierte Zusammenhang zwi schen Farbe der Ohrscheiben einer Henne und der Farbe der Eierschale scheint empirisch nicht belegbar zu sein [9][10][11]. So kommt es zwischen Hennen einer Rasse zu einer beacht lichen Varianz bezüglich der Färbung ihrer Eier, ebenso wie es für indivi duelle Hennen eine Varianz der Fär bung in Abhängigkeit ihres Alters gibt [10].…”