Spermatozoal transcripts expression levels are predictive of semen quality and conception rate in bulls ( Bos taurus )

Parthipan, Sivashanmugam; Selvaraju, Sellappan; Somashekar, Lakshminarayana; Arunachalam, A.; Sivaram, M.; Ravindra, J.P.

doi:10.1016/j.theriogenology.2017.04.042

Cited by 44 publications

(26 citation statements)

References 46 publications

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“…In conclusion, the outcomes of the kinetic parameters for post-thaw cryopreserved spermatozoa show that the values improved with DGC separation system and have increased the occurrence of sperm with intact DNA by reducing the values obtained from DFI analysis. This further validates that spermatozoa from the CDGC group sustained minimal damage to their DNA following separation and cryopreservation and is suitable for implementation in various artificial insemination procedures (Joint FAO, 2005;Fraser, 2017;Parthipan et al, 2017). By having sperm with high DNA integrity, the sperm conserved its repair mechanism and was able to reduce the detrimental effects of cryodamage.…”

Section: Resultssupporting

confidence: 63%

Improvement of Post-Thaw Sperm Kinematics and DNA Integrity of Cross-Bred Bovine Sperm by Incorporating DGC as Selection Method Prior to Cryopreservation

Ismail¹,

Osman²,

Yusof³

et al. 2017

JAS

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The aim of this study was to assess post-thaw sperm quality following initial sperm selection using density gradient centrifugation (DGC) prior to cryopreservation. Ejaculates from four mature Charolais cross Kedah-Kelantan bulls were collected using artificial vagina at IBVK Pahang, Malaysia. The ejaculates were aliquoted into 3 groups: non-cryopreserved group (NC); control group of cryopreserved sperm without DGC (ND) and treatment group of sperm undergoing DGC sperm selection before cryopreservation (CDGC). Prior to analysis, samples from both cryopreserved groups were thawed at 37 °C for 30 sec. All samples were analysed for kinematics parameters, viability and compromise in DNA integrity (evaluated as DNA Fragmentation Index, DFI). All kinematics parameters were analysed using computer aided sperm analysis (CASA). Results indicated significant (p < 0.05) kinematics parameter changes for all parameters of velocity (VCL, VSL, VAP) and progression (WOB, LIN, ALH and BCF). Unfortunately, changes in spermatozoa straightness were insignificant (STR) F(2, 68) = 1.004, p = 0.371. Spermatozoa viability had increased by 26.2% (p < 0.01) following the treatment. DFI revealed the treatment group recorded a significant reduction in DFI value (0.17% fragmented DNA). In conclusion, DGC sperm selection prior to cryopreservation reduced the effects of cryodamage and showed an improvement in post-thaw sperm quality, thus reducing the occurrence of asthenozoospermia in populations of frozen-thawed cross-bred bovine spermatozoa.

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Section: Resultssupporting

confidence: 63%

Improvement of Post-Thaw Sperm Kinematics and DNA Integrity of Cross-Bred Bovine Sperm by Incorporating DGC as Selection Method Prior to Cryopreservation

Ismail¹,

Osman²,

Yusof³

et al. 2017

JAS

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“…Since the mitochondria are TA B L E 2 Effect of freezing on the bovine epididymal sperm motility patterns using CASA in vitro the main source of energy in the most mammal's sperm, the motion parameters changes related to lateral displacements of the sperm is because of impaired mitochondria following cryopreservation (Hung, Miller, Meyers, & VandeVoort, 2008). Therefore, in our study, Although the use of spermatozoal transcripts as markers of sperm quality and fertility (Jodar et al, 2016;Parthipan et al, 2017) has been developed, the studies focusing on spermatozoal transcripts and ultralow temperatures are limited even though most of the RNA studies emphasis on frozen specimens (Card, Krieger, Kaproth, & Sartini, 2017;Chen et al, 2015;Ganguly et al, 2013;Kopeika, Thornhill, & Khalaf, 2015; Zeng et al, 2014).…”

Section: Discussionmentioning

confidence: 88%

“…Studies have recognized and quantified the sperm cryogenic changes characterised by sperm function and fertility alterations. Although the use of spermatozoal transcripts as markers of sperm quality and fertility (Jodar et al., 2016; Parthipan et al., 2017) has been developed, the studies focusing on spermatozoal transcripts and ultralow temperatures are limited even though most of the RNA studies emphasis on frozen specimens (Card, Krieger, Kaproth, & Sartini, 2017; Chen et al., 2015; Ganguly et al., 2013; Kopeika, Thornhill, & Khalaf, 2015; Zeng et al., 2014).…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation and its effects on motility and gene expression patterns and fertilizing potential of bovine epididymal sperm

Nazari

Ahmadi

Hamid

et al. 2020

Veterinary Medicine & Sci

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Despite encountering new challenges in using epididymal sperm recovered from cauda epididymides, this accessible and, in some species, worthwhile sample makes inevitable the further development of a suitable cryopreservation protocol. In this study, sperm was recovered from the epididymis of 4°C overnight stored slaughtered bulls' testes and the effects of cryopreservation on the bovine epididymal sperm motility (with CASA) and gene expression patterns (with quantitative Real time‐PCR) were evaluated. Moreover the fertilizing potential of cryopreserved epididymal sperm was used in in vitro fertilization (IVF). After freezing and thawing of epididymal sperm, total and slow progressive sperm motility, VCL, VAP, MAD, ALH and BCF were significantly decreased (p < .05), while in the parameters of fast progressive motility, VSL, LIN, WOB and STR there were not any significant variations in the frozen sperm compared to fresh (non‐frozen) counterpart. The assessment of abundance of transcripts encoding motility (TSSK6) and fertility (PRM1 and PRM2)‐related genes in epididymal sperm, showed that these transcripts were affected by freezing especially in slow progressive motility status (p < .01). Furthermore, cleavage and blastocyst rate did not present any significant differences between bovine embryos produced in vitro by fresh or frozen‐thawed epididymal sperm. It can be concluded that epididymal sperm has enough freezability after overnight testes storage, and cryopreservation could not affect the percentage of in vitro produced embryos in spite of the changes of relative abundance of some transcripts and direction progressive motility pattern of sperm.

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“…A number of quantitative trait loci and genetic variants are already known to be associated with male fertility traits, including semen quality and cryosurvival. Recently, proteomic approaches have started to be used to investigate semen freezability and identify sperm proteins, mRNA and miRNA transcripts that could be used as biomarkers of bull semen and fertility traits (Parthipan et al, 2017). For instance, Mostek et al (2018) observed differences in the protein profile and carbonylation level between high and low-quality bull semen ejaculates.…”

Section: Ta B L Ementioning

confidence: 99%

Variation in sperm cryosurvival is not modified by replacing the cryoprotectant glycerol with ethylene glycol in bulls

Gororo

Makuza

Chidzwondo

et al. 2020

Reprod Domestic Animals

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Contents Breed and sire differences in sperm cryosurvival have been noted, with negative implications for sperm cryobanking and assisted reproduction programmes. This study hypothesized that these differences could be modified by using lower molecular weight cryoprotectants. Therefore, the effect of replacing glycerol (GLY) with ethylene glycol (EG) on differential cryosurvival of semen from two Sanga cattle breeds (Mashona vs. Tuli) was determined. Three to five ejaculates were collected from each of ten bulls (3‐8 years) by electro‐ejaculation, diluted in three Tris‐egg yolk extenders (Triladyl®, 7% GLY‐based and 7% EG‐based) and evaluated for sperm motility, viability and morphology at three time periods (fresh – 0 hr, pre‐freeze – 4 hr and post‐thaw). Tuli bulls produced larger (11.8 ± 0.31 ml vs. 8.5 ± 0.38 ml) and more concentrated ejaculates of lower fresh semen quality. Breeds differed across time for motility and morphology, but not viability. Mashona bull semen had significantly higher motility and normal morphology values at each sampling time. Bulls classified as poor freezers had lower concentration (0.70 ± 0.09 × 109 sperm/ml vs. 1.37 ± 0.10 × 109 sperm/ml), sperm motility index (SMI, 35.0 ± 3.4 % vs. 67.8 ± 2.1 %) and viability (69.7 ± 1.1 % vs. 75.7 ± 1.0 %) compared to good freezers. Maintenance of semen quality by GLY and EG did not differ between breeds, poor and good freezers, or age groups. The interaction breed by extender across time did not reach statistical significance for all variables. The study revealed that bull and breed variation in sperm quality and cryosurvival is not modified by replacing GLY with EG, suggesting that cryostress tolerance of sperm may be under control of mechanisms other than differential response to GLY cytotoxicity.

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Spermatozoal transcripts expression levels are predictive of semen quality and conception rate in bulls ( Bos taurus )

Cited by 44 publications

References 46 publications

Improvement of Post-Thaw Sperm Kinematics and DNA Integrity of Cross-Bred Bovine Sperm by Incorporating DGC as Selection Method Prior to Cryopreservation

Improvement of Post-Thaw Sperm Kinematics and DNA Integrity of Cross-Bred Bovine Sperm by Incorporating DGC as Selection Method Prior to Cryopreservation

Cryopreservation and its effects on motility and gene expression patterns and fertilizing potential of bovine epididymal sperm

Variation in sperm cryosurvival is not modified by replacing the cryoprotectant glycerol with ethylene glycol in bulls

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