Spermine facilitates recovery from drought but does not confer drought tolerance in transgenic rice plants expressing Datura stramonium S-adenosylmethionine decarboxylase

Peremartí, Ariadna; Bassié, Ludovic; Christou, Paul; Capell, Teresa

doi:10.1007/s11103-009-9470-5

Cited by 65 publications

(30 citation statements)

References 54 publications

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“…This could be due to the translational or post-translational regulation of these genes, as previously shown (Carbonell and Blázquez 2009;Alcázar et al 2010;Mattoo et al 2010;Shao et al 2011). Also, in previous studies, overexpression of the PA biosynthetic genes such as SAMDC or SPDS has been shown to bring about very limited accumulation of Spd and/or Spm (Wen et al 2008;Peremarti et al 2009), which may be ascribed to tight homeostatic regulation of these PA at the cellular level. Therefore, the mature-fruit abscission is accompanied by decreased OeSPD1 and OeSAMDC1 gene expression, parallel to SAMDC activity …”

Section: Mature-fruit Abscission Changes Pa Biosynthesis Transcript Pmentioning

confidence: 74%

“…Changes in plant PA biosynthetic enzyme activities and gene expression are regulated separately, depending on physiological and developmental conditions (Bagni and Tassoni 2001;Kusano et al 2008;Carbonell and Blázquez 2009;Shao et al 2011), and SAMDC was found to be an important enzyme in the regulation of PA homeostasis (Kusano et al 2008;Mattoo et al 2010). As the cellular PA content can be regulated at the transcriptional level, it is possible to modulate the endogenous PA level via overexpression of the PA biosynthetic genes, as has been previously revealed (Mehta et al 2002;Wen et al 2008;Peremarti et al 2009;Nambeesan et al 2010).…”

Section: Introductionmentioning

confidence: 72%

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Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Gil-Amado

Gómez-Jiménez

2011

Planta

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Exogenous ethylene and some inhibitors of polyamine biosynthesis can induce mature-fruit abscission in olive, which could be associated with decreased nitric oxide production as a signaling molecule. Whether H₂O₂ also plays a signaling role in mature-fruit abscission is unknown. The possible involvement of H₂O₂ and polyamine in ethylene-induced mature-fruit abscission was examined in the abscission zone and adjacent cells of two olive cultivars. Endogenous H₂O₂ showed an increase in the abscission zone during mature-fruit abscission, suggesting that accumulated H₂O₂ may participate in abscission signaling. On the other hand, we followed the expression of two genes involved in the polyamine biosynthesis pathway during mature-fruit abscission and in response to ethylene or inhibitors of ethylene and polyamine. OeSAMDC1 and OeSPDS1 were expressed differentially within and between the abscission zones of the two cultivars. OeSAMDC1 showed slightly lower expression in association with mature-fruit abscission. Furthermore, our data show that exogenous ethylene or inhibitors of polyamine encourage the free putrescine pool and decrease the soluble-conjugated spermidine, spermine, homospermidine, and cadaverine in the olive abscission zone, while ethylene inhibition by CoCl₂ increases these soluble conjugates, but does not affect free putrescine. Although the impact of these treatments on polyamine metabolism depends on the cultivar, the results confirm that the mature-fruit abscission may be accompanied by an inhibition of S-adenosyl methionine decarboxylase activity, and the promotion of putrescine synthesis in olive abscission zone, suggesting that endogenous putrescine may play a complementary role to ethylene in the normal course of mature-fruit abscission.

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Section: Mature-fruit Abscission Changes Pa Biosynthesis Transcript Pmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 72%

Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Gil-Amado

Gómez-Jiménez

2011

Planta

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“…It has been found that an Arabidopsis mutant plant that does not produce Spm was hypersensitive to drought stress (Kusano et al 2007). Spd plays a role as intermediate by indirectly maintaining high Spm content during abiotic stress, but accumulated Spd content in plants might get also converted into Put (Roychoudhury et al 2011;Peremarti et al 2009). Higher (Spd ?…”

Section: Discussionmentioning

confidence: 99%

Exogenously applied spermidine improves drought tolerance in creeping bentgrass associated with changes in antioxidant defense, endogenous polyamines and phytohormones

Zhou

Peng

et al. 2014

Plant Growth Regul

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Improvement of endogenous polyamine (PA) levels by exogenous spermidine (Spd) application in leaves may promote drought tolerance of plants. In this study, leaves of creeping bentgrass (cv. 'Penn-A4') were pretreated with 0.2 mmol Spd every another day for three cycles before water was limited for 15 days in a growth chamber. The application of exogenous Spd elevated the accumulation of endogenous PA, including putrescine, Spd and spermine in leaves of creeping bentgrass under drought stress. Exogenous Spd effectively alleviated the damage effects from drought stress, as demonstrated by lower O 2 Á-generation rate, H 2 O 2 and malondialdehyde content, higher relative water content, chlorophyll content and antioxidant enzyme activities (superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase) as compared to untreated plants. Additionally, the pretreatment with Spd resulted in a lower accumulation of indole-3-acetic acid (IAA) and gibberellin A 3 (GA 3 ) after 15 days of drought stress, but an unchanged accumulation of abscisic acid compared to the untreated ones. These results suggest that ameliorating drought stress through exogenously applied Spd may be associated with increased antioxidant protection capability and after a longer stress treatment with changed IAA and GA 3 accumulation level on account of improved endogenous PA content. In addition, drought tolerance mechanisms related to PA regulation of phytohormones are very complex and deserve further investigation.

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“…Increases in the Spd levels in the transgenic lines were moderate (1.3-to 2-fold higher), yet the susceptibility to B. cinerea was greatly increased. Moderate changes in PA levels, especially increases in Spd, have been shown to cause significant physiological changes in transgenic Arabidopsis, rice (Oryza sativa), and pear (Pyrus communis) plants overexpressing SpdSyn and SAMdc genes (Kasukabe et al, 2004;Wen et al, 2008;Peremarti et al, 2009). Unsuccessful attempts to obtain stable transgenic potato plants expressing a potato SAMdc under the CaMV35S promoter, while SAMdc antisense lines led to a range of stunted phenotypes also support this contention (Kumar et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Polyamines Attenuate Ethylene-Mediated Defense Responses to Abrogate Resistance to Botrytis cinerea in Tomato

et al. 2011

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Transgenic tomato (Solanum lycopersicum) lines overexpressing yeast spermidine synthase (ySpdSyn), an enzyme involved in polyamine (PA) biosynthesis, were developed. These transgenic lines accumulate higher levels of spermidine (Spd) than the wild-type plants and were examined for responses to the fungal necrotrophs Botrytis cinerea and Alternaria solani, bacterial pathogen Pseudomonas syringae pv tomato DC3000, and larvae of the chewing insect tobacco hornworm (Manduca sexta). The Spd-accumulating transgenic tomato lines were more susceptible to B. cinerea than the wild-type plants; however, responses to A. solani, P. syringae, or M. sexta were similar to the wild-type plants. Exogenous application of ethylene precursors, S-adenosylMet and 1-aminocyclopropane-1-carboxylic acid, or PA biosynthesis inhibitors reversed the response of the transgenic plants to B. cinerea. The increased susceptibility of the ySpdSyn transgenic tomato to B. cinerea was associated with down-regulation of gene transcripts involved in ethylene biosynthesis and signaling. These data suggest that PA-mediated susceptibility to B. cinerea is linked to interference with the functions of ethylene in plant defense.

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Spermine facilitates recovery from drought but does not confer drought tolerance in transgenic rice plants expressing Datura stramonium S-adenosylmethionine decarboxylase

Cited by 65 publications

References 54 publications

Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Exogenously applied spermidine improves drought tolerance in creeping bentgrass associated with changes in antioxidant defense, endogenous polyamines and phytohormones

Polyamines Attenuate Ethylene-Mediated Defense Responses to Abrogate Resistance to Botrytis cinerea in Tomato

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