Spexin and Metformin Comparative Ameliorated Ovarian and Liver Function Changes in Letrozole-Induced Polycystic Ovary Syndrome in Rats (Histological, Biochemical, Immunohistochemical and Morphometric study)

Abulfadle, Khaled Abdelfattah; Edrees, Husam M.; Hassan, Nancy Husseiny; Mohammed, Heba Osama

doi:10.21608/ejh.2021.76972.1486

Cited by 3 publications

(3 citation statements)

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“…The current work demonstrated a remarkable rise in caspase-3 immune expression in the ovaries of PCOS rats. This finding coincided with Abulfadle et al, 55 who attributed such increase to elevated blood TNF-α levels and increased oxidative stress halted the growth of follicles by inducing apoptosis in granulosa cells. This led to a decline in the quality of oocytes and the gradual degeneration of the follicles.…”

Section: Discussionsupporting

confidence: 86%

Ameliorative potential of rosmarinic acid in a rat model of polycystic ovary syndrome: Targeting MCP‐1 and VEGF: A histological, immunohistochemical, and biochemical study

Shalaby,

Elshamy

et al. 2024

Cell Biochemistry & Function

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Polycystic ovary syndrome (PCOS) is a multidisciplinary endocrinopathy that affects women of reproductive age. It is characterized by menstrual complications, hyperandrogenism, insulin resistance, and cardiovascular issues. The current research investigated the efficacy of rosmarinic acid in letrozole‐induced PCOS in adult female rats as well as the potential underlying molecular mechanisms. Forty female rats were divided into the control group, the rosmarinic acid group (50 mg/kg per orally, po) for 21 days, PCOS group; PCOS was induced by administration of letrozole (1 mg/kg po) for 21 days, and rosmarinic acid‐PCOS group, received rosmarinic acid after PCOS induction. PCOS resulted in a marked elevation in both serum luteinizing hormone (LH) and testosterone levels and LH/follicle‐stimulating hormone ratio with a marked reduction in serum estradiol and progesterone levels. A marked rise in tumor necrosis factor‐α (TNF‐α), interleukin‐1β, monocyte chemotactic protein‐1, and vascular endothelial growth factor (messenger RNA) in the ovarian tissue was reported. The histological analysis displayed multiple cystic follicles in the ovarian cortex with markedly thin granulosa cell layer, vacuolated granulosa and theca cell layers, and desquamated granulosa cells. Upregulation in the immune expression of TNF‐α and caspase‐3 was demonstrated in the ovarian cortex. Interestingly, rosmarinic acid ameliorated the biochemical and histopathological changes. In conclusion, rosmarinic acid ameliorates letrozole‐induced PCOS through its anti‐inflammatory and antiangiogenesis effects.

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Section: Discussionsupporting

confidence: 86%

Ameliorative potential of rosmarinic acid in a rat model of polycystic ovary syndrome: Targeting MCP‐1 and VEGF: A histological, immunohistochemical, and biochemical study

Shalaby,

Elshamy

et al. 2024

Cell Biochemistry & Function

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“…Similarly, Abulfadle et al (54) reported that metformin ameliorated hepatic toxicity produced by PCOS, causing an improvement in its biochemical changes because metformin had anti-inflammatory activity. Moreover, Rababa'h et al (55) reported that metformin antagonizes insulin resistance and endocrine disorders in the PCOS animal model via triggering activated mitogen protein kinase .…”

Section: Discussionmentioning

confidence: 95%

Erythropoietin, Myoinositol and Metformin Modulate Insulin Sensitivity Indices, Pancreatic Beta Cell Mass and Hepatocellular Changes in a Rat Model of Polycystic Ovary Syndrome

mohammed

Elhaliem

Fanous

et al. 2022

Ain Shams Medical Journal

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Background: Polycystic ovary syndrome (PCOS) is a common health problem in females throughout their reproductive age. Common symptoms of PCOS are hyperandrogenemia, menstrual disorders, and infertility. Metabolic disorders like insulin resistance and steatotic liver are also common. Metformin is most commonly used in the management of hyperglycemia. Erythropoietin and myoinositol were reported to improve insulin sensitivity.Objective: This study aimed to compare the potential therapeutic role of erythropoietin, myoinositol, and the widely used metformin drug against hyperinsulinemia and hepatic injury of the letrozole-induced PCOS model in rats. Materials and methods:Fifty female Wistar rats were classified equally into five groups: The control group and the PCO model group. PCO model was induced by letrozole in a dose of 0.5 mg/kg daily for 3 weeks. Then rats with PCO were administrated: erythropoietin in (EPO/PCO) group, myoinositol in (MYO/PCO) group, or metformin in (MET/PCO) group for the following 21 days. Biochemical and histological studies on the liver and pancreas were done. Immunohistochemical staining with GLUT-1 and insulin was done.Results: PCO rats developed insulin resistance with pancreatic β cell degeneration, hepatocellular injury, and upregulation of hepatic GLUT-1. Metformin, erythropoietin, and myoinositol restored β-cell mass, decreased hyperinsulinemia, and attenuated hepatocellular degeneration via the reduction of stress-induced hepatic GLUT-1. However, erythropoietin was the most effective one of them. Conclusion:Erythropoietin was more effective than metformin and myoinositol in decreasing hyperinsulinemia and attenuation of hepatocellular injury associated with the letrozole-induced model of PCOS.

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“…A mean of 15 readings was estimated from 5 serial sections from slides of each animal in each group (the total was 120 measurements/group). H&E staining was applied on every 20th section of the ovary so that each section was separated by a distance of approximately 50-60 µm from the next 20th section to avoid counting the same follicle twice [18]. According to [19], only oocyte-containing ovarian follicles were considered and follicles were classified as primordial, primary, secondary, and Graafian follicles.…”

Section: Morphometric Analysismentioning

confidence: 99%

Melatonin Mitigates Cisplatin-Induced Ovarian Dysfunction via Altering Steroidogenesis, Inflammation, Apoptosis, Oxidative Stress, and PTEN/PI3K/Akt/mTOR/AMPK Signaling Pathway in Female Rats

et al. 2022

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Ovarian damage and fertility impairment are major side effects of chemotherapy in pre-menopausal cancer patients. Cisplatin is a widely used chemotherapeutic drug. The present study was designed to assess the ameliorative effects of melatonin as an adjuvant for fertility preservation. Thirty-two adult female Wistar rats were divided randomly into four equal groups: Control, Melatonin, Cisplatin (CP) treated, and CP + Melatonin treated. The cisplatin-treated group showed decreased body and ovarian weights, decreased serum E2 and AMH, increased serum LH and FSH, reduced ovarian levels of SOD, CAT, GSH, and TAC, and increased ovarian MDA. The histopathological examination of the cisplatin-treated group showed deleterious changes within ovarian tissue in the form of damaged follicles and corpus luteum, hemorrhage, and inflammatory infiltrates with faint PAS reaction in zona pellucida, increased ovarian collagen deposition, and marked expression of caspase-3 immune reaction in granulosa and theca cells, stroma, and oocytes. Alongside, there was a significant downregulation in the mRNA expression of steroidogenic enzymes, IL10, AMPK, PI3K, AKT, mTOR, and PTEN, while TGF-β1, IL1β, IL6, TNF-α, NF-Kβ, P53, p38-MAPK, JNK, and FOXO3 mRNA expressions were upregulated in cisplatin-treated rats’ ovarian tissue. Coadministration of cisplatin-treated rats with melatonin reversed these changes significantly. In conclusion, melatonin’s antioxidant, anti-inflammatory, and anti-apoptotic activities could modulate ovarian disturbances induced by cisplatin and preserve fertility.

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Spexin and Metformin Comparative Ameliorated Ovarian and Liver Function Changes in Letrozole-Induced Polycystic Ovary Syndrome in Rats (Histological, Biochemical, Immunohistochemical and Morphometric study)

Cited by 3 publications

References 0 publications

Ameliorative potential of rosmarinic acid in a rat model of polycystic ovary syndrome: Targeting MCP‐1 and VEGF: A histological, immunohistochemical, and biochemical study

Ameliorative potential of rosmarinic acid in a rat model of polycystic ovary syndrome: Targeting MCP‐1 and VEGF: A histological, immunohistochemical, and biochemical study

Erythropoietin, Myoinositol and Metformin Modulate Insulin Sensitivity Indices, Pancreatic Beta Cell Mass and Hepatocellular Changes in a Rat Model of Polycystic Ovary Syndrome

Melatonin Mitigates Cisplatin-Induced Ovarian Dysfunction via Altering Steroidogenesis, Inflammation, Apoptosis, Oxidative Stress, and PTEN/PI3K/Akt/mTOR/AMPK Signaling Pathway in Female Rats

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