Spheroid formation of mesenchymal stem cells on chitosan and chitosan-hyaluronan membranes

Huang, Guo-Shiang; Dai, Lien-Guo; Yen, B. Linju; Hsu, Shan‐hui

doi:10.1016/j.biomaterials.2011.05.092

Cited by 198 publications

(221 citation statements)

References 53 publications

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“…On the polycationic chitosan membrane, A-MSCs aggregated through noncontrolled cell motility and readily merged to form aggregates, but the size distribution displayed high variability. 71,72 Nanoculture and poly(ethylene glycol)-micropatterned plates promoted homogeneity and increased the viability of BM-MSC aggregates. 34,73 To enhance initial aggregate size homogeneity and cell viability, thermal lifting surface incorporating with endogenous ECMs was also applied to form BM-MSC aggregates.…”

Section: Methods To Generate 3d Msc Aggregatesmentioning

confidence: 99%

“…34,35,50 Independent of the aggregation method, increased compactness has been reported in aggregates of A-MSC or BM-MSC over the prolonged culture. 5,35,50,61,72 In long-term culture, the proliferation capacity of placental and A-MSC aggregates generated on chitosan membranes was reduced. 71,72 The incorporation of hyaluronan in chitosan membrane increased the survival and proliferation ability presumably due to increased cell migration and matrix synthesis.…”

Section: -115mentioning

confidence: 99%

“…5,35,50,61,72 In long-term culture, the proliferation capacity of placental and A-MSC aggregates generated on chitosan membranes was reduced. 71,72 The incorporation of hyaluronan in chitosan membrane increased the survival and proliferation ability presumably due to increased cell migration and matrix synthesis. 72 It has been suggested that cell proliferation is inversely related to aggregate size due to diffusion limitations, and large aggregates have reduced proliferation potential compared to small ones.…”

Section: -115mentioning

confidence: 99%

“…71,72 The incorporation of hyaluronan in chitosan membrane increased the survival and proliferation ability presumably due to increased cell migration and matrix synthesis. 72 It has been suggested that cell proliferation is inversely related to aggregate size due to diffusion limitations, and large aggregates have reduced proliferation potential compared to small ones. 107,116 However, the MSC aggregates are heterogeneous in both molecular profiles and biomechanical microenvironments.…”

Section: -115mentioning

confidence: 99%

See 3 more Smart Citations

Three-Dimensional Aggregates of Mesenchymal Stem Cells: Cellular Mechanisms, Biological Properties, and Applications

Sart

Tsai

et al. 2014

Tissue Engineering Part B: Reviews

331

362

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Mesenchymal stem cells (MSCs) are primary candidates in cell therapy and tissue engineering and are being tested in clinical trials for a wide range of diseases. Originally isolated and expanded as plastic adherent cells, MSCs have intriguing properties of in vitro self-assembly into three-dimensional (3D) aggregates reminiscent of skeletal condensation in vivo. Recent studies have shown that MSC 3D aggregation improved a range of biological properties, including multilineage potential, secretion of therapeutic factors, and resistance against ischemic condition. Hence, the formation of 3D MSC aggregates has been explored as a novel strategy to improve cell delivery, functional activation, and in vivo retention to enhance therapeutic outcomes. This article summarizes recent reports of MSC aggregate self-assembly, characterization of biological properties, and their applications in preclinical models. The cellular and molecular mechanisms underlying MSC aggregate formation and functional activation are discussed, and the areas that warrant further investigation are highlighted. These analyses are combined to provide perspectives for identifying the controlling mechanisms and refining the methods of aggregate fabrication and expansion for clinical applications.

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Section: Methods To Generate 3d Msc Aggregatesmentioning

confidence: 99%

Section: -115mentioning

confidence: 99%

Section: -115mentioning

confidence: 99%

Section: -115mentioning

confidence: 99%

See 2 more Smart Citations

Three-Dimensional Aggregates of Mesenchymal Stem Cells: Cellular Mechanisms, Biological Properties, and Applications

Sart

Tsai

et al. 2014

Tissue Engineering Part B: Reviews

331

362

View full text Add to dashboard Cite

show abstract

“…21,22 DPSCs are an attractive postnatal stem cell source as they are easily accessible, can be easily expanded ex vivo, and exhibit multipotency and regenerative capacity, whereas extraction of MSCs requires an invasive procedure and MSCs exhibit less expansion capacity than do DPSCs. [23][24][25] Murine DPSCs are derived from neural crest origin and express the epithelial-mesenchymal transition (EMT) genes, Twist, Snail, and Slug. 21 These EMT genes have been shown to be important for inhibition of chondrogenesis both in vitro and in vivo, whereas downregulation of EMT genes enhances chondrogenic differentiation.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Chondrogenic Differentiation of Dental Pulp Stem Cells Using Nanopatterned PEG-GelMA-HA Hydrogels

Nemeth

Janebodin

Yuan

et al. 2014

Tissue Engineering Part A

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Direct 3D Printed Biomimetic Scaffolds Based on Hydrogel Microparticles for Cell Spheroid Growth

Zhang

Cong

Osi

et al. 2020

Adv Funct Materials

122

124

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Biocompatible hydrogel inks with shear‐thinning, appropriate yield strength, and fast self‐healing are desired for 3D bioprinting. However, the lack of ideal 3D bioprinting inks with outstanding printability and high structural fidelity, as well as cell‐compatibility, has hindered the progress of extrusion‐based 3D bioprinting for tissue engineering. In this study, novel self‐healable pre‐cross‐linked hydrogel microparticles (pcHμPs) of chitosan methacrylate (CHMA) and polyvinyl alcohol (PVA) hybrid hydrogels are developed and used as bioinks for extrusion‐based 3D printing of scaffolds with high fidelity and biocompatibility. The pcHμPs display excellent shear thinning when injected through a syringe and subsequently self‐heal into gels as shear forces are removed. Numerical simulations indicate that the pcHμPs experience a plug flow in the nozzle with minimal disturbance, which favors a steady and continuous printing. Moreover, the pcHμPs show a self‐supportive yield strength (540 Pa), which is critical for the fidelity of printed constructs. A series of biomimetic constructs with very high aspect ratio and delicate fine structures are directly printed by using the pcHμP ink. The 3D printed scaffolds support the growth of bone‐marrow‐derived mesenchymal stem cells and formation of cell spheroids, which are most important for tissue engineering.

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Spheroid formation of mesenchymal stem cells on chitosan and chitosan-hyaluronan membranes

Cited by 198 publications

References 53 publications

Three-Dimensional Aggregates of Mesenchymal Stem Cells: Cellular Mechanisms, Biological Properties, and Applications

Three-Dimensional Aggregates of Mesenchymal Stem Cells: Cellular Mechanisms, Biological Properties, and Applications

Enhanced Chondrogenic Differentiation of Dental Pulp Stem Cells Using Nanopatterned PEG-GelMA-HA Hydrogels

Direct 3D Printed Biomimetic Scaffolds Based on Hydrogel Microparticles for Cell Spheroid Growth

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