Spheroid model for functional osteogenic evaluation of human adipose derived stem cells

Gurumurthy, B M; Bierdeman, Patrick C.; Janorkar, Amol V.

doi:10.1002/jbm.a.35974

Cited by 46 publications

(45 citation statements)

References 35 publications

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“…Thus, the biomimetic bioreactor environment provided results closer to the in vivo findings than the 2D culture. Furthermore, as compared to spheroid cell cultures, often used as 3D models (e.g., ), explant cultures applied in the present study provided the native tissue structure and composition closely resembling the in vivo situation.…”

Section: Discussionmentioning

confidence: 89%

Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants

Zvicer

Mišković‐Stanković

Obradović

2018

J Biomedical Materials Res

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In this work, functional characterization of biomaterials concerning potential application as articular cartilage implants was performed by using a biomimetic bioreactor with dynamic compression in the physiological regime (10% strain, 0.84 Hz frequency, 1 h on/1 h off). Specifically, two alginate types with low (LG) and high (HG) guluronic/mannuronic residue ratios with electrochemically synthesized silver nanoparticles (AgNPs) were evaluated. HG Ag/alginate hydrogels were clearly indicated as potential candidates due to better initial mechanical properties as compared to LG hydrogels (dynamic compression modulus of ~60 vs. ~40 kPa) as well as the mechanical stability displayed during 7 days of dynamic compression. Cytotoxicity studies in 3D bovine cartilage explant cultures under dynamic compression have shown negligible effects as compared to standard 2D monolayers of bovine chondrocytes where moderate cytotoxicity was observed. Finally, experimental and mathematical modeling studies revealed different mechanisms of AgNP release under physiological‐like bioreactor conditions as compared to static conditions. Overall, the results clearly demonstrate bioreactor advantages in characterization and selection of candidate biomaterials as well as potentials to bridge the in vitro‐in vivo gap. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 755–768, 2019.

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Section: Discussionmentioning

confidence: 89%

Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants

Zvicer

Mišković‐Stanković

Obradović

2018

J Biomedical Materials Res

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“…The reliability of the procedure has previously been reported in works analysing the mechanical behaviour of foot adipose tissues (Gurumurthy et al. ; Fontanella et al. ).…”

Section: Discussionmentioning

confidence: 70%

The characteristics of the lobular arrangement indicate the dynamic role played by the infrapatellar fat pad in knee kinematics

et al. 2019

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The infrapatellar fat pad (IFP) is an intracapsular but extrasynovial structure, located between the patellar tendon, the femoral condyles and the tibial plateau. It consists of white adipose tissue, organised in lobules defined by thin connective septa. The aim of this study is the morphometric and ultrasonographic analysis of IFP in subjects without knee pathology during flexion-extension movements. The morphometric study was conducted on 20 cadavers (15M, 5F, mean age 80.2 years). Ultrasound was performed on 24 volunteers with no history of knee diseases (5M, 19F, mean age: 45 years). The characteristics of the adipose lobules near the patellar tendon and in the deep portion of the IFP were evaluated. Numerical models were provided, according to the size of the lobules. At histological examination, the adipose lobules located near the patellar tendon were larger (mean area 12.2 mm 2 AE 5.3) than those at a deeper level (mean area 1.34 mm 2 AE 0.7, P < 0.001) and the thickness of the septa of the deepest adipose lobules (mean value 0.35 mm AE 0.32) was greater than that of the superficial one (mean value 0.29 mm AE 0.25, P < 0.001). At ultrasound, the IFP was seen to be composed of very large lobules in the superficial part (mean area 0.29 cm 2 AE 0.17 in extension), with a significant reduction in flexion (mean area 0.12 cm 2 AE 0.07, P < 0.01). The deep lobules were smaller (mean area 0.11 cm 2 AE 0.08 in extension) and did not change their values (mean area 0.19 cm 2 AE 0.52 in flexion, P > 0.05). In the sagittal plane, the reduction of thickness of the superficial layer (with large adipose lobules) during flexion was 20.6%, whereas that of the deep layer (with small adipose lobules) was 1.3%. Numerical simulation of vertical loads, corresponding to flexion of the knee, showed that stress mainly developed within the interlobular septa and opposed bulging of the lobules. The characteristics of the lobular arrangement of the IFP (large lobules with superficial septa in the superficial part and small lobules with thick septa in the deep one), significant changes in the areas and perimeters of the superficial lobules, and the reduced thickness of the superficial layer during flexion all indicate the dynamic role played by the IFP in knee kinematics.

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“…Mineralization was also higher for the 3D spheroids when compared to the 2D model. In conclusion, 3D spheroids were found to be a better system for hASC differentiation with respect to the standard culture methods [118].…”

Section: Skeletal System Regenerationmentioning

confidence: 81%

Genetically Engineered Elastin-based Biomaterials for Biomedical Applications

Santos

Serrano-Dúcar

González‐Valdivieso

et al. 2020

CMC

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Protein-based polymers are some of the most promising candidates for a new generation of innovative biomaterials as recent advances in genetic-engineering and biotechnological techniques mean that protein-based biomaterials can be designed and constructed with a high degree of complexity and accuracy. Moreover, their sequences, which are derived from structural protein-based modules, can easily be modified to include bioactive motifs that improve their functions and material-host interactions, thereby satisfying fundamental biological requirements. The accuracy with which these advanced polypeptides can be produced, and their versatility, self-assembly behavior, stimuli-responsiveness and biocompatibility, means that they have attracted increasing attention for use in biomedical applications such as cell culture, tissue engineering, protein purification, surface engineering and controlled drug delivery. The biopolymers discussed in this review are elastin-derived protein-based polymers which are biologically inspired and biomimetic materials. This review will also focus on the design, synthesis and characterization of these genetically encoded polymers and their potential utility for controlled drug and gene delivery, as well as in tissue engineering and regenerative medicine.

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Spheroid model for functional osteogenic evaluation of human adipose derived stem cells

Cited by 46 publications

References 35 publications

Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants

Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants

The characteristics of the lobular arrangement indicate the dynamic role played by the infrapatellar fat pad in knee kinematics

Genetically Engineered Elastin-based Biomaterials for Biomedical Applications

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