Spheroids in radiobiology and photodynamic therapy

Establishment of Human Colon Cancer Cell Lines from Fresh Tumors versus Xenografts: Comparison of Success Rate and Cell Line Features

“…CT329X12 was also more resistant to 5-FU in three-dimensional conformation than CT329 but more sensitive to L-OHP whatever the in vitro model. Spheroids were used in chemosensitivity tests because threedimensional spheroids were primarily used to study tumor resistance to radiotherapy and chemotherapy (32). More recently, Weaver et al showed that polarized three-dimensional architecture confers upon the tumor cell resistance to apoptosis-inducing agents, including chemotherapy drugs and ligation of cell death receptors (33).…”

Spheroid-based 3D Cell Cultures Enable Personalized Therapy Testing and Drug Discovery in Head and Neck Cancer

“…Kadletz et al could not detect any radiationrelated cytotoxicity for a variation of dosages in different HNSCC cell lines (52). However, sensitivity and resistance to irradiation is mainly influenced by effectiveness of DNA repair, apoptosis, oxygen supply and cell-cell contacts, features that, in spheroid cell culture, are similar to tumors in vivo (29,65,66). In conclusion, it is plausible that the so-called chemo-and radiation resistance, in the context of spheroid cell culture, is actually the result of more in vivolike cell functionality.…”

Prostate and Colon Cancer Stem Cells as a Target for Anti-Cancer Drug Development

“…This model is more closely related to original tumors with respect to cell morphology, metabolic and proliferative gradients, oxygen and drug penetration, cell-cell junctions, kinases activation and other parameters, compared to the cancer cell monolayers (Friedrich et al, 2009). Spheroid cells have an enhanced resistance to many of the commonly used anticancer drugs (Dessoize et al, 2000;Yoshida et al, 2008), showing dramatically lower cytotoxicity against 3D cancer spheroids compared to monolayer cultures, and exhibit chemoresistance which recapitulates this resistant phenotype in vivo (Dubessy et al, 2000;Durand et al, 2001;Friedrich et al, 2009). Increased resistance of spheroid cells to ionizing radiation was first demonstrated by Sutherland and colleagues (Inch et al, 1970;Sutherland et al, 1971).…”