Sphingadienine-1-phosphate levels are regulated by a novel glycoside hydrolase family 1 glucocerebrosidase widely distributed in seed plants

Abstract

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

The putative obtusifoliol 14α‐demethylase OsCYP51H3 affects multiple aspects of rice growth and development

“…Disruption of Os3BGlu6 in rice resulted in dwarfism, lower ABA content in leaves, drought‐sensitivity, lower photosynthesis rate and higher intercellular CO 2 concentration (Wang et al, 2020). Further study indicated that Os3BGlu6 can regulate the level of ceramides containing sphingadienine, influencing the regulation of sphingadienine‐1‐phosphate levels and subsequent improvement of drought tolerance via stomatal closure in rice (Koga et al, 2021). Previous study showed that OsSUS3 overexpression could significantly improve hull size and grain weight by dynamically regulating cell division and starch accumulation in the transgenic rice (Fan et al, 2019).…”

Dietary Fungal Glucosylceramide and Ceramide Reduce the Formation of Aberrant Crypt Foci in 1,2-Dimethylhydrazine-Treated Mice: Differences in the Role of Glucosylceramide and Ceramide

“…In a previous study, noting that shiitake mushroom (Lentinula edodes) is rich in a GlcCer species, (4E,8E)-N-D-2′-hydroxypalmitoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8sphingadienine [Glc-d19:2(4E,8E,9Me)-h16:0] (fungal GlcCer), we used this fungus to develop a new method for purifying the GlcCer using ethanol precipitation and high performance liquid chromatography (HPLC). 22 Furthermore, because human glycoside hydrolase family (GH) 30 glucocerebrosidase (GCase) hydrolyzes plant GlcCer to Cer 23 and rice GH1 GCase hydrolyzes fungal GlcCer to Cer, 24 we also developed a new method to produce large amounts of a Cer species, [d19:2(4E,8E,9Me)-h16:0] (fungal Cer) from this purified fungal GlcCer using human GH 30 GCase. 22 In the present study, to clarify the roles of sphingolipids in intestinal impairment in vivo, we investigated the effects of these fungal GlcCer and Cer on ACF formation and colon inflammation.…”