Sphingobacterium micropteri sp. nov. and Sphingobacterium litopenaei sp. nov., isolated from aquaculture water

Abstract

Two novel bacterial strains, designated as DN00404T and DN04309T, were isolated from aquaculture water and characterized by using a polyphasic taxonomic approach. Cells of strains DN00404T and DN04309T were Gram-stain-negative, aerobic, non-motile, oxidase-positive and catalase-positive. Cells of DN00404T were short rod-shaped and those of DN04309T were long rod-shaped. Strain DN00404T was found to grow at 15–37 °C (optimum, 25–30 °C), at pH 6.0–11.0 (optimum, pH 7.5) and in 0–2.0 % (w/v) NaCl (optimum, 1.0 %). Strain DN04309T was found to grow at 15–45 °C (optimum, 20–37 °C), at pH 5.5–11.0 (optimum, 7.5) and in 0–4.0 % (w/v) NaCl (optimum, 0.5 %). Phylogenetic analyses based on 16S rRNA gene and genome sequences revealed that the two strains belonged to the genus Sphingobacterium and were distinct from all known species of this genus. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between the two strains and between each of the two strains and related type strains of this genus were well below the recognized thresholds of 95.0–96.0 % ANI and 70.0 % dDDH for species delineation. The genomic DNA G+C contents of strains DN00404T and DN04309T were 41.6 and 36.0 mol%, respectively. The respiratory quinone in both strains was identified as MK-7, and their major fatty acids were iso-C15 : 0 and summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c), which were similar to those of other species of this genus. The two major fatty acids C16 : 0 and iso-C17 : 0 3-OH were also found in strain DN00404T. Based on genotypic and phenotypic characteristics, two novel species of the genus Sphingobacterium are proposed: Sphingobacterium micropteri sp. nov. with DN00404T (=GDMCC 1.1865T=KACC 21924T) as the type strain and Sphingobacterium litopenaei sp. nov. with DN04309T (=GDMCC 1.1984T=KCTC 82348T) as the type strain.

Sphingobacterium oryzagri sp. nov., isolated from rice paddy soil

“…S3). The results of the polar lipid and isoprenoid quinone analysis obtained from the present study were consistent with the patterns observed in members of the genus Sphingobacterium [3–10].…”

“…At the time of writing, the genus represented by the type species Sphingobacterium spiritivorum, comprised 68 validly published species (https://lpsn.dsmz.de/). Sphingobacterium species have been discovered from diverse environments, including aquaculture water [3], food-waste compost [4], potato stem [5], raw milk [6], rhizosphere [7] and soil [8][9][10]. Members of the genus are characterized by aerobic, Gram-stain-negative, rod-shaped, nonspore-forming and yellow-pigmented bacteria, containing high concentrations of sphingophospholipids in their cells.…”

Pseudomonas oligotrophica sp. nov., a Novel Denitrifying Bacterium Possessing Nitrogen Removal Capability Under Low Carbon–Nitrogen Ratio Condition

“…Genomic DNA of strains JM10B5a T was extracted from fresh cells, and the 16S rRNA gene sequence was amplified using the universal primers (27F/1492R) as described previously (Zhang et al, 2021 ). Sequencing was performed by GENEWIZ, Inc. (Suzhou, China).…”

Marivirga aurantiaca sp. nov., a halophilic nitrite-reducing bacterium, isolated from intertidal surface sediments

“…The genomic DNA of S37H4 T was extracted using the HiPure Bacterial DNA Kit (Guangzhou Magen Biotechnology) according to the manufacturer’s instructions. The 16S rRNA gene sequence was amplified using universal primers (27F/1492R) as described previously, and the PCR products were sequenced by GENEWIZ (Suzhou, Jiangsu, PR China) [10]. Similar sequences of 16S rRNA genes were searched for in the EzBioCloud database (https://www.ezbiocloud.net/) [11].…”