Sphingobium nicotianae sp. nov., isolated from tobacco soil

Abstract

Bacterial strain H33T was isolated from tobacco plant soil and was characterized using a polyphasic taxonomy approach. Strain H33T was a Gram-stain-negative, rod-shaped, non-motile and strictly aerobic bacterium. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of the up-to-date bacterial core gene set (92 protein clusters) indicated that H33T belongs to the genus Sphingobium . Strain H33T showed the highest 16S rRNA gene sequence similarity to Sphingobium xanthum NL9T (97.2%) and showed 72.3–80.6 % average nucleotide identity and 19.7–29.2 % digital DNA–DNA hybridization identity with the strains of other species of the genus Sphingobium . Strain H33T grew optimally at 30°C, pH 7 and could tolerate 0.5 % (w/v) NaCl. The isoprenoid quinones were ubiquinone-9 (64.1%) and ubiquinone-10 (35.9%). Spermidine was the major polyamine. The major fatty acids of H33T were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The polar lipid profile consisted of a mixture of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, sphingoglycolipid, two unidentified lipids, two unidentified glycolipids, two unidentified aminoglycolipids and an unidentified phospholipid. The genomic DNA G+C content of H33T was 64.9 mol%. Based on the phylogenetic and phenotypic data, H33T was considered a representative of a novel species in the genus Sphingobium . We propose the name Sphingobium nicotianae sp. nov., with H33T (=CCTCC AB 2022073T=LMG 32569T) as the type strain.

Sphingobium cyanobacteriorum sp. nov., isolated from fresh water

“…The genus Sphingobium, of the family Sphingomonadaceae within the order Sphingomonadales, was first described by Takeuchi et al [1] with the type species Sphingobium yanoikuyae. As of February 2024, this genus accommodates 46 species with validly published names (LPSN, https://lpsn.dsmz.de) that have been isolated from diverse habitats, such as contaminated soil [2], groundwater [3] and wastewater treatment plants [4]. The common characteristics of this genus are being Gram-negative, rod-shaped, motile or non-motile and strictly aerobic [1].…”