Sphingolipids as differential regulators of cellular signalling processes

Pyne, Susan; Tolan, Deborah; Conway, Ann-Marie; Pyne, Nigel J.

doi:10.1042/bst0250549

Cited by 26 publications

(17 citation statements)

References 29 publications

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“…The breakdown products (such as ceramide, sphingosine and SPP) are believed to regulate multiple cellular processes, including growth regulation, differentiation and apoptosis, to name but a few. 88,207,249,250 In contrast to mammals, it appears that the majority of S. cerevisiae sphingolipids are stable and are not turned over in haploid cells during vegetative growth. 290 Thus, the production of potential sphingolipid signalling molecules in S. cerevisiae, including DHS, PHS, DHS-1-P, PHS-1-P and ceramide, 61,107,290 are most likely derived from de novo synthesis, not from the turnover of preformed sphingolipids.…”

Section: Cellular Location Sites Of Synthesis and Functions Of Sphinmentioning

confidence: 99%

Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiae

Daum

Lees

Bard

et al. 1998

Yeast

580

380

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The yeast Saccharomyces cerevisiae is a powerful experimental system to study biochemical, cell biological and molecular biological aspects of lipid synthesis. Most but not all genes encoding enzymes involved in fatty acid, phospholipid, sterol or sphingolipid biosynthesis of this unicellular eukaryote have been cloned, and many gene products have been functionally characterized. Less information is available about genes and gene products governing the transport of lipids between organelles and within membranes, turnover and degradation of complex lipids, regulation of lipid biosynthesis, and linkage of lipid metabolism to other cellular processes. Here we summarize current knowledge about lipid biosynthetic pathways in S. cerevisiae and describe the characteristic features of the gene products involved. We focus on recent discoveries in these fields and address questions on the regulation of lipid synthesis, subcellular localization of lipid biosynthetic steps, cross‐talk between organelles during lipid synthesis and subcellular distribution of lipids. Finally, we discuss distinct functions of certain key lipids and their possible roles in cellular processes. © 1998 John Wiley & Sons, Ltd.

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Section: Cellular Location Sites Of Synthesis and Functions Of Sphinmentioning

confidence: 99%

Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiae

Daum

Lees

Bard

et al. 1998

Yeast

580

380

View full text Add to dashboard Cite

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“…One particular sphingolipid, sphingosine 1-phosphate (SPP), 1 which belongs to a group of platelet-derived lipid mediators, has recently emerged as an important lipid messenger. SPP binds to prototypical members of the orphan endothelial differentiation gene (EDG) family that includes EDG-1, ARG16/h218, and EDG-3 (1-6), which are G i -linked receptors stimulating the p42/44 mitogen-activated protein kinase (p42/44 MAPK) pathway.…”

mentioning

confidence: 99%

Cystic Fibrosis Transmembrane Regulator Regulates Uptake of Sphingoid Base Phosphates and Lysophosphatidic Acid

Boujaoude¹,

Bradshaw-Wilder²,

Mao³

et al. 2001

Journal of Biological Chemistry

135

103

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Sphingolipids have been implicated in the regulation of cell growth, differentiation, and programmed cell death. Sphingosine 1-phosphate (SPP) has recently emerged as an important lipid messenger and a ligand for the endothelial differentiation gene receptor family of proteins through which it mediates its biologic effects. Recent studies in Saccharomyces cerevisiae in our laboratory implicated the yeast oligomycin resistance gene (YOR1), a member of the ATP binding cassette family of proteins, in the transport of SPP. The cystic fibrosis transmembrane regulator is a unique member of the ATP binding cassette transporter family and has high homology with YOR1. We therefore set out to investigate if this member of the family can regulate SPP transport. We demonstrate that C127/cystic fibrosis transmembrane regulator (CFTR) cells, expressing wild type CFTR, exhibited significantly higher uptake of sphingosine 1-phosphate than either cells expressing a mutant CFTR C127/⌬F508 or C127/mock-transfected cells. This effect was specific, dose-dependent, and competed off by dihydrosphingosine 1-phosphate and lysophosphatidic acid. There was no difference in uptake of sphingosine, C 16 -ceramide, sphingomyelin, lysophingomyelin, phosphatidylcholine, lysophosphatidylcholine, or phosphatidic acid among the different cell lines. Pretreatment with forskolin or isobutylmethylxanthine to stimulate cAMP did not affect the uptake in any of the cell lines. Moreover, we found that mitogen-activated protein kinase activation by SPP was less responsive in C127/CFTR as compared with C127/mock-transfected cells, suggesting that uptake of SPP by CFTR may divert it from interacting with its cell surface receptors and attenuate signaling functions. Taken together, these data implicate CFTR in uptake of SPP and the related phosphorylated lipids dihydrosphingosine 1-phosphate and lysophosphatidic acid. This uptake influences the availability of SPP to modulate biologic activity via endothelial differentiation gene receptors. These studies may have important implications to cystic fibrosis.

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“…70 This was part of the ancient European Mediterranean culture of fire, 71 that took advantage of the summer water stress which meant that large quantities of dead biomass could not fully decompose and tended to accumulate in forests or scrubland until ignited by lightning. This accounts for the fact that Mediterranean woodlands have always coexisted with natural fires.…”

Section: Land-use and Livestock Breeding As A Crucial Metabolic Hingementioning

confidence: 99%

The Basic environmental history

2015

Choice Reviews Online

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Ecological economics is enabling economic and environmental historians to enhance their understanding of economic growth, by placing it in a broader perspective of biophysical interactions between nature and society. In this chapter, several ongoing researches and historical debates are examined from this standpoint such as the missing role of energy carriers in GDP growth, the socio-metabolic profiles of past and present societies, the pre-industrial 'Smithian' responses to 'Malthusian' traps, the role of efficient land-use in breeding livestock to increase agricultural yields, the reasons why the Industrial Revolution began in a high wage and cheap energy economy, the first globalization as a socio-metabolic watershed, and the question of whether there was a general crisis of biomass energies at the coming of fossil fuels era. Research discussing long-term socio-metabolic transitions may contribute to our understanding of how economic growth actually occurred, and which ecological impacts affected the Earth's life-support systems. Equally, these projects leave room for the institutional settings or ruling actors needed to explain why growth has happened and by whom. Far from naturalising history, the use of ecology in the explanation of human history historialises ecology.

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Sphingolipids as differential regulators of cellular signalling processes

Cited by 26 publications

References 29 publications

Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiae

Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiae

Cystic Fibrosis Transmembrane Regulator Regulates Uptake of Sphingoid Base Phosphates and Lysophosphatidic Acid

The Basic environmental history

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