Sphingolipids in immature and mature soybeans

Ohnishi, Masao; Fujino, Yuichi

doi:10.1007/bf02535357

Cited by 70 publications

(56 citation statements)

References 21 publications

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“…TLC on silica gel G with chloroformmethanol (95: 12, v/v) of CM and G1CM isolated from rice grains gave at least three spots,6) Rfvalues of which were identical with the authentic ones prepared from soybean seeds 9 ) and Aspergillus oryzae. 18 ) When oligoglycosylceramides from rice grains were used for TLC (Fig.…”

Section: Confirmation Of Sph Ingo Lip Idsmentioning

confidence: 73%

“…The mass spectra of the second major peak (22%) gave the base peak at mjz 204 and notable ions at mj z 1332 (M -15) indicating the molecular weight, at mjz 1050 and 297 due to the sphingoid moiety, and at mjz 528 and 570 derived from the component fatty acid. 9 ,zO,Zl) From these ions the peak species was identified as 1-0-glucosyl-N"2' -hydroxylignoceroyl-4-hydroxysphingenine. Likewise, mass spectrometric data of the other peak lipids were assayed and are summarized in Table V.…”

Section: Principal Molecular Species Of CM and G 1 Cmmentioning

confidence: 99%

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Molecular Species of Ceramide and Mono-, Di-, Tri-, and Tetraglycosylceramide in Bran and Endosperm of Rice Grains

Fujino

Ohnishi

1985

Agricultural and Biological Chemistry

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Section: Confirmation Of Sph Ingo Lip Idsmentioning

confidence: 73%

Section: Principal Molecular Species Of CM and G 1 Cmmentioning

confidence: 99%

Molecular Species of Ceramide and Mono-, Di-, Tri-, and Tetraglycosylceramide in Bran and Endosperm of Rice Grains

Fujino

Ohnishi

1985

Agricultural and Biological Chemistry

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“…Similarly, in the few cases where cis-trans isomerization of long-chain bases has been reported, the trans isomer oft 18:1 is typically more abundant than the cis isomer (e.g. 8,[23][24][25]. In addition, the composition of intact glucocerebroside molecular species detected in the leaf and plasma membrane of rye were similar.…”

Section: Gc/ms Identification Of Glucocerebroside Molecular Speciesmentioning

confidence: 89%

“…In a previous study (18), glucocerebrosides were reported to constitute 16 mol% of the plasma membrane lipid of cells of noncold-acclimated rye leaves. It was also suggested that this lipid may be of importance in altering cryostability of rye plasma membrane (18,30 (29), Phaseolus vulgaris (5), and spinach (25) as well as seeds of Azuki bean (23), pea (1 1), and soybean (24) consist almost entirely of saturated 2-hydroxy fatty acids. Similarly, in the few cases where cis-trans isomerization of long-chain bases has been reported, the trans isomer oft 18:1 is typically more abundant than the cis isomer (e.g.…”

Section: Gc/ms Identification Of Glucocerebroside Molecular Speciesmentioning

confidence: 99%

Analysis of Glucocerebrosides of Rye (Secale cereale L. cv Puma) Leaf and Plasma Membrane

Cahoon¹,

Lynch²

1991

Plant Physiol.

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Glucocerebrosides of whole rye (Secale cerale L. cv Puma) leaf and plasma membrane were analyzed using gas chromatography-mass spectrometry and gas chromatography following hydrolysis or as intact molecules purified by reverse-phase high performance liquid chromatography. Fatty acids of acid-hydrolyzed leaf and plasma membrane glucocerebrosides consisted of >98 weight percent saturated and monounsaturated 2-hydroxy fatty acids which contained 16 to 26 carbon atoms. The major fatty acids detected were 2-hydroxynervonic acid (24:1h), 2-hydroxylignoceric acid (24:0h), 2-hydroxyerucic acid (22:1h), and 2-hydroxybehenic acid (22:0h). Long-chain bases of alkalinehydrolyzed glucocerebrosides consisted primarily of cis-trans isomers of the trihydroxy base 4-hydroxysphingenine (t18:1) and the dihydroxy base sphingadienine (dl8:2) with lesser amounts of 4-hydroxysphinganine (t18:0) and isomers of sphingenine (d18: 1). Intact, underivatized glucocerebroside molecular species of rye leaf and plasma membrane were separated into more than 30 molecular species using reverse-phase HPLC. The molecular species composition of leaf and plasma membrane were quantitatively and qualitatively similar. The major molecular species was 24:1h-t18:1 which constituted nearly 40 weight percent of leaf and plasma membrane extracts. Several other species including 22:1h-t18:1, 24:1h-t18:1 (isomer), 22:0h-t18:1, 24:1h-d18:2, and 24:0h-t18:1 each comprised 4 to 8% of the total. It is anticipated that the high performance liquid chromatography procedure developed in this study to separate intact, underivatized lipid molecular species will be useful in future studies of the physical properties and biosynthesis of plant glucocerebrosides.Glucocerebrosides are a class of glycosphingolipids that have been reported to constitute less than 1% of the total lipid extract of plant tissues including runner bean leaves (29) and rice bran (8). Recently, however, glucocerebrosides have been identified as a major lipid component of the plasma membrane and tonoplast of cells of several plant species. Plasma membrane isolated from rye (18) and barley (27) leaves, oat root (28), oat coleoptile (28), and mung bean hypocotyl (33)

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“…CMH is known to be widespread in plants, although the content is usually low (4)(5)(6)17). It has been reported that plasma membrane and tonoplast contain CMH (8,12,15,30).…”

Section: Discussionmentioning

confidence: 99%

Lipid Composition of Plasma Membranes and Tonoplasts Isolated from Etiolated Seedlings of Mung Bean (Vigna radiata L.)

Yoshida¹,

Uemura²

1986

Plant Physiol.

193

102

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The lipid composition of plasma membranes and tonoplasts from etiolated mung bean hypocotyls was examined in detail. Phospholipids, sterols, and ceramide monohexoside(s) were the major lipid classes in both membranes. The content of phospholipids on a protein basis was higher in the tonoplast, but the content of total sterols was similar in both membranes. Accordingly, the sterol to phospholipid molar ratio in the plasma membrane was higher than that of the tonoplast. Phosphatidylethanolamine and phosphatidylcholine comprised the major phospholipids in both membranes. Phosphatidylinositol, phosphatidylserine, and phosphatidylglycerol were identified as minor phospholipid components. The content of phosphatidylinositol and phosphatidylglycerol was relatively high in the tonoplast, comprising 11 and 5% of the total phospholipids, respectively. Although special care was taken against the degradative action of phospholipase D and phosphatidic acid phosphatase during the isolation of these membranes, by adding EDTA, EGTA, KF, choline, and ethanolamine to the homogenizing medium, significant amounts of phosphatidic acid, about 15% of the total phospholipids, were detected in the plasma membrane. On the other hand, the content of phosphatidic acid in tonoplasts and other membrane fractions was very low. This fact may indicate that high levels of phosphatidic acid occur naturally in plasma membranes. Phosphatidylglycerol in both membranes and phosphatidylinositol in the tonoplast contained high levels of palmitic acid, which comprised more than 50% of the total fatty acids. Significant differences were observed in the sterol compositions of plasma membranes and tonoplasts. More than 90% of the sterols in the plasma membrane were unesterified, while the tonoplast was enriched in glycosylated sterols, especially acylated sterylglycosides. Ceramide monohexoside was found to be specifically located in these membranes, in particular, in the tonoplast, in which it comprised nearly 17% of the total lipids. is quite limited regarding the lipid composition of plasma membranes and tonoplasts, which also possess very important physiological functions including in the former, transport (22), cell wall biosynthesis (14), hormone action (24), phytochrome responses (18), and disease resistance (27), and in the latter, cellular compartmentation of lytic enzymes, acids, ions, and secondary products (I 1, 13). In addition, these membranes have been shown to play an important role in cold acclimation (29,31,33), freezing injury (26), and chilling stress (32).Recently we have established isolation methods for plasma membranes and tonoplasts from etiolated mung bean hypocotyls (34,35). In the present study, we have analyzed the lipid composition of both membranes and compared them. Plasma membranes are characterized by their high content of phosphatidic acid and tonoplasts by their high content of ceramide monohexoside. MATERIALS AND METHODSPlant Materials. Seeds of mung bean (Vigna radiata [L] Wilczek) were imbibed and germinate...

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Sphingolipids in immature and mature soybeans

Cited by 70 publications

References 21 publications

Molecular Species of Ceramide and Mono-, Di-, Tri-, and Tetraglycosylceramide in Bran and Endosperm of Rice Grains

Molecular Species of Ceramide and Mono-, Di-, Tri-, and Tetraglycosylceramide in Bran and Endosperm of Rice Grains

Analysis of Glucocerebrosides of Rye (Secale cereale L. cv Puma) Leaf and Plasma Membrane

Lipid Composition of Plasma Membranes and Tonoplasts Isolated from Etiolated Seedlings of Mung Bean (Vigna radiata L.)

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