Sphingomonas changnyeongensis sp. nov. isolated from the Hapcheon–Changnyeong barrage area in the Nakdong river

Abstract

The novel bacterial strain C33T was isolated from a freshwater sample collected from the Hapcheon–Changnyeong barrage. The Gram-negative, motile, yellow-pigmented strain C33T was characterized as a rod-shaped and strictly aerobic bacterium. A 16S-rRNA phylogenetic analysis revealed that this strain was most closely related to Sphingomonas changbaiensis V2M44T, Sphingomonas tabacisoli X1-8T, and Sphingomonas flavalba ZLT-5T with 97.1, 97.0, and 95.0 % 16S-rRNA sequence similarities, respectively. The genomic DNA GC content of strain C33T was estimated at 65.0 mol%. The average nucleotide identity of strain C33T relative to S. changbaiensis V2M44T and S. flavalba ZLT-5T was found to be 77.0 and 75.6%, with average amino-acid identities of 69.9, and 66.7%, and the digital DNA–DNA hybridization values of 21.3 and 17.7 %, respectively. The cells grew at 19–37 °C and pH 6–9 with 0–0.5 % (w/v) NaCl (optimum: 28 °C, pH 6.5, and 0 % NaCl). The major component identified in the polyamine pattern was sym-homospermidine, and the main ubiquinone was Q-10. The predominant polar lipids characterized were diphophatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, and sphingoglycolipid. Iso-C15 : 0, C15 : 0 anteiso, and summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) were found to be the primary cellular fatty acids in strain C33T. Based on these genotypic and phenotypic characteristics, strain C33T was classified as a novel species of the genus Sphingomonas ; and the name Sphingomonas changnyeongensis sp. nov. is proposed (=KACC 21511T=JCM 33880T).

Niabella defluvii sp. nov., isolated from influent water of a wastewater treatment plant

“…S4). Isoprenoid quinones were examined by isocratic high-performance liquid chromatography using Nova-Pak C18 reverse-phase columns (particle size, 4 µm; Waters) [33, 34]. The mobile phase consisted of a methanol–isopropyl ether solution (3 : 1; v/v) flowing at a rate of 0.7 ml min −1 .…”

Niveibacterium microcysteis sp. nov., isolated from a cyanobacterial bloom sample

“…To identify the bacterium, freshwater samples collected from HCW (35° 35′ 32.5″ N 128° 21′ 25.4″ E) were filtered through a 22 µm filter paper. The samples were then serially diluted and spread on Reasoner's 2A (R2A) agar (0.05 g yeast extract, 0.05 g proteose peptone No.3, 0.05 g casamino acid, 0.05 g d -glucose, 0.05 g soluble starch, 0.03 g sodium pyruvate, 0.03 g K 2 HPO 4 , 0.005 g MgSO 4 and 1.5 g agar powder in 100 ml distilled water, pH 7) plates and incubated for 3 days at 30 °C [14]. Strain HC41 T was isolated from one of these plates.…”

Sphingomonas psychrotolerans sp. nov., isolated from root surface of Leontopodium leontopodioides in the Tianshan Mountains, Xinjiang, China

“…The genus Sphingomonas was originally described by Yabuuchi et al [1] and then emended by Busse et al [2] and Chen et al [3]. At the time of writing, the genus Sphingomonas consists of more than 190 recognized species (www.bacterio.net), including the recently described species Sphingomonas aliaeris [4], Sphingomonas sabuli [5] and Sphingomonas changnyeongensis [6]. Members of the genus Sphingomonas are Gram-negative, yellow-pigmented, aerobic, non-fermentative, non-sporeforming, non-motile or motile rods and are characterized chemotaxonomically by the presence of ubiquinone Q-10 and 2-hydroxy fatty acids [7, 8].…”