Sphingosinicella soli sp. nov., isolated from an alkaline soil in Korea

Abstract

A Gram-negative, motile, rod-shaped bacterial strain, KSL-125 T , was isolated from an alkaline soil from Kwangchun, Korea, and its taxonomic position was investigated in a polyphasic study. Strain KSL-125 T grew optimally at 30 6C, at pH 7.5-8.0 and in the presence of 0.5 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain KSL-125 T joins the cluster comprising the two Sphingosinicella species at a bootstrap resampling value of 100 %. The values for similarity between the 16S rRNA gene sequence of strain KSL-125 T and those of the type strains of the two Sphingosinicella species were 98.9-99.0 %. Strain KSL-125 T contained Q-10 as the predominant ubiquinone and C 17 : 1 v6c, C 16 : 1 v7c and/or iso-C 15 : 0 2-OH and C 18 : 1 v7c as the major fatty acids. The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and an unidentified phospholipid. The DNA G+C content was 65.1 mol%. Strain KSL-125 T was distinguishable from the two recognized Sphingosinicella species on the basis of differential phenotypic properties, DNA-DNA relatedness data and repetitive-sequence-based PCR genomic fingerprinting patterns. The phenotypic, phylogenetic and genetic data showed that strain KSL-125 T represents a novel species of the genus Sphingosinicella, for which the name Sphingosinicella soli sp. nov. is proposed. The type strain is KSL-125 T (5KCTC 12482 T 5DSM 17328 T ).

Phenanthrene biodegradation by sphingomonads and its application in the contaminated soils and sediments: A review

“…This family comprises of four well-known PAH-degrading genera, namely; Sphingomonas (Busse et al, 2003;Chen et al, 2012Chen et al, , 2013Takeuchi et al, 2001;Yabuuchi et al, 2002Yabuuchi et al, , 1999, Sphingobium (Busse et al, 2003;Takeuchi et al, 2001), Novosphingobium (Busse et al, 2003;Takeuchi et al, 2001), Sphingopyxis (Busse et al, 2003;Takeuchi et al, 2001;Yoon et al, 2008, which make up the sphingomonads (Kosako et al, 2000). Currently, ten other genera have been established in this family, including Sphingosinicella Maruyama et al, 2006;Yasir et al, 2010), Sandarakinorhabdus (Gich and Overmann, 2006), Stakelama (Chen et al, 2010;Thawng et al, 2013), Sphingorhabdus , Zymomonas (Glaeser and K€ ampfer, 2014), Sandaracinobacter (Yurkov et al, 1997), Sphingomicrobium (K€ ampfer et al, 2012), Blastomonas (Hiraishi et al, 2000), Pacificamonas (Liu et al, 2014) and Parasphingopyxis (Uchida et al, 2012).…”

Sphingoaurantiacus polygranulatus gen. nov., sp. nov., isolated from high-Arctic tundra soil, and emended descriptions of the genera Sandarakinorhabdus, Polymorphobacter and Rhizorhabdus and the species Sandarakinorhabdus limnophila, Rhizorhabdus argentea and Sphingomonas wittichii

“…T (Geueke et al, 2007), the strain with the highest 16S rRNA gene sequence similarity, Sandarakinorhabdus limnophila DSM 17366 T (Gich & Overmann, 2006) and Polymorphobacter multimanifer JCM 18140 T (Fukuda et al, 2014), with which the novel strain forms a monophyletic cluster, and other strains with relatively high similarity, Sphingomonas wittichii DSM 6014 T (Yabuuchi et al, 2001), Sphingosinicella microcystinivorans KCTC 12019 T (Maruyama et al, 2006), Sphingosinicella soli KCTC 12482 T (Yoon et al, 2008), Hephaestia caeni DSM 25527…”

Stakelama pacifica gen. nov., sp. nov., a new member of the family Sphingomonadaceae isolated from the Pacific Ocean

“…Differentiation of physiological and biochemical characteristics between strain JLT832 T and closely related species within the family Sphingomonadaceae Taxa: 1, JLT832 T ; 2, Sphingosinicella microcystinivorans JCM 13185 T ; 3, Sphingosinicella xenopeptidilytica DSM 17130 T ; 4, Sphingosinicella soli DSM 17328 T ; 5, Novosphingobium panipatense CCM 7472 T ; 6, Novosphingobium mathurense CCM 7473 T ; 7, Novosphingobium subarcticum SMCC B0478 T ; 8, Zymomonas species. Data in columns were taken from the following sources: 1-4, Maruyama et al (2006), Geueke et al (2007) and Yoon et al (2008); 5-6, Gupta et al (2009); 7, Nohynek et al (1996) and Lim et al (2007);8, De Ley & Swings (1976) and Swings & De Ley (1977). All strains were negative for Gram stain, gelatin hydrolysis and indole production.…”