spICP-MS characterisation of released silver nanoparticles from (nano)textile products

Rujido-Santos, Iria; Busto, M. Estela del Castillo; Abad-Álvaro, Isabel; Herbello-Hermelo, Paloma; Bermejo–Barrera, Pilar; Barciela‐Alonso, María Carmen; Goneaga-Infante, Heidi; Moreda–Piñeiro, Antonio

doi:10.1016/j.sab.2022.106505

Cited by 4 publications

(3 citation statements)

References 55 publications

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“…Therefore, the transfer process could be expressed as Then, the degradation of NPs could be expressed as where k di1 represents the degradation rate constant in the phagolysosome and k di2 represents the degradation rate constant in the endolysosome. At the same time, Ag ions were depurated, which therefore the kinetic change of Ag ions could be expressed as below: Overall, the kinetic change of particulate Ag and total Ag could be inferred as As for the particle number concentration, previous studies reported that AgNPs with size below 10 nm could not be determined by SP-ICP-MS. − Since the dissolution of NPs mainly occurred in the lysosomal compartment, we assumed that the decrease of particle number concentration was induced by its degradation in the lysosome. The degradation time for each NP could be defined as the duration needed for NP to degrade from pristine size (37 nm) to 10 nm.…”

Section: Methodsmentioning

confidence: 99%

Tracking the Cellular Degradation of Silver Nanoparticles: Development of a Generic Kinetic Model

Wang,

Wang

2024

ACS Nano

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Understanding the degradation of nanoparticles (NPs) after crossing the cell plasma membrane is crucial in drug delivery designs and cytotoxicity assessment. However, the key factors controlling the degradable kinetics remain unclear due to the absence of a quantification model. In this study, subcellular imaging of silver nanoparticles (AgNPs) was used to determine the intracellular transfer of AgNPs, and single particle ICP-MS was utilized to track the degradation process. A cellular kinetic model was subsequently developed to describe the uptake, transfer, and degradation behaviors of AgNPs. Our model demonstrated that the intracellular degradation efficiency of AgNPs was much higher than that determined by mimicking testing, and the degradation of NPs was highly influenced by cellular factors. Specifically, deficiencies in Ca or Zn primarily decreased the kinetic dissolution of NPs, while a Ca deficiency also resulted in the retardation of NP transfer. The biological significance of these kinetic parameters was strongly revealed. Our model indicated that the majority of internalized AgNPs dissolved, with the resulting ions being rapidly depurated. The release of Ag ions was largely dependent on the microvesicle-mediated route. By changing the coating and size of AgNPs, the model results suggested that size influenced the transfer of NPs into the degradation process, whereas coating affected the degradation kinetics. Overall, our developed model provides a valuable tool for understanding and predicting the impacts of the physicochemical properties of NPs and the ambient environment on nanotoxicity and therapeutic efficacy.

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Section: Methodsmentioning

confidence: 99%

Tracking the Cellular Degradation of Silver Nanoparticles: Development of a Generic Kinetic Model

Wang,

Wang

2024

ACS Nano

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“…Colloidal stability, as well as aggregation or agglomeration and dissolution stage of NPs, affects the biological interaction, toxicity, and environmental fate of NPs [58][59][60]. An ideal method would analyze aggregation and dissolution in situ [61].…”

Section: Determination Of the Particle Size (Stability Solubility Agg...mentioning

confidence: 99%

Basic and advanced spectrometric methods for complete nanoparticles characterization in bio/eco systems: current status and future prospects

Borowska

Jankowski

2023

Anal Bioanal Chem

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The use of engineered nanoparticles in the environment and human life has increased in the last 20 years. The risk assessment concerning application of nanomaterials in biological systems requires their thorough characterization. Understanding the correlations between physicochemical properties of nanoparticles concerning not only the size, particle size distribution, number concentration, degree of aggregation, or agglomeration but also solubility, stability, binding affinity, surface activity, chemical composition, and nanoparticle synthesis yield allows their reliable characterization. Thus, to find the structure-function/property relationship of nanoparticles, multifaceted characterization approach based on more than one analytical technique is required. On the other hand, the increasing demand for identification and characterization of nanomaterials has contributed to the continuous development of spectrometric techniques which enables for their qualitative and quantitative analysis in complex matrices giving reproducible and reliable results. This review is aimed at providing a discussion concerning four main aspects of nanoparticle characterization: nanoparticle synthesis yield, particle size and number concentration, elemental and isotopic composition of nanoparticles, and their surface properties. The conventional and non-conventional spectrometric techniques such as spectrophotometry UV-Vis, mass spectrometric techniques working in conventional and single-particle mode, or those based on optical emission detection systems are described with special emphasis paid on their advantages and drawbacks. The application and recent advances of these methods are also comprehensively reviewed and critically discussed. Graphical abstract

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“…Besides all the properties of silver ions, also in the form of Ag 0 nanoparticles, silver shows tremendous potential as an antibacterial agent [8][9][10][11], currently used in numerous consumer and medical products [12][13][14]. However, silver nanoparticles (AgNPs) have several drawbacks that must be studied [15].…”

Section: Introductionmentioning

confidence: 99%

Microalga Broths Synthesize Antibacterial and Non-Cytotoxic Silver Nanoparticles Showing Synergy with Antibiotics and Bacterial ROS Induction and Can Be Reused for Successive AgNP Batches

Pernas-Pleite,

Conejo-Martínez,

Fernández Freire

et al. 2023

IJMS

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The era of increasing bacterial antibiotic resistance requires new approaches to fight infections. With this purpose, silver-based nanomaterials are a reality in some fields and promise new developments. We report the green synthesis of silver nanoparticles (AgNPs) using culture broths from a microalga. Broths from two media, with different compositions and pHs and sampled at two growth phases, produced eight AgNP types. Nanoparticles harvested after several synthesis periods showed differences in antibacterial activity and stability. Moreover, an evaluation of the broths for several consecutive syntheses did not find relevant kinetics or activity differences until the third round. Physicochemical characteristics of the AgNPs (core and hydrodynamic sizes, Z-potential, crystallinity, and corona composition) were determined, observing differences depending on the broths used. AgNPs showed good antibacterial activity at concentrations producing no or low cytotoxicity on cultured eukaryotic cells. All the AgNPs had high levels of synergy against Escherichia coli and Staphylococcus aureus with the classic antibiotics streptomycin and kanamycin, but with ampicillin only against S. aureus and tetracycline against E. coli. Differences in the synergy levels were also dependent on the types of AgNPs. We also found that, for some AgNPs, the killing of bacteria started before the massive accumulation of ROS.

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spICP-MS characterisation of released silver nanoparticles from (nano)textile products

Cited by 4 publications

References 55 publications

Tracking the Cellular Degradation of Silver Nanoparticles: Development of a Generic Kinetic Model

Tracking the Cellular Degradation of Silver Nanoparticles: Development of a Generic Kinetic Model

Basic and advanced spectrometric methods for complete nanoparticles characterization in bio/eco systems: current status and future prospects

Microalga Broths Synthesize Antibacterial and Non-Cytotoxic Silver Nanoparticles Showing Synergy with Antibiotics and Bacterial ROS Induction and Can Be Reused for Successive AgNP Batches

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